Transcript: Mouse XM_006512697.3

PREDICTED: Mus musculus lactation elevated 1 (Lace1), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Afg1l (215951)
Length:
2489
CDS:
566..1579

Additional Resources:

NCBI RefSeq record:
XM_006512697.3
NBCI Gene record:
Afg1l (215951)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006512697.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000121014 GCCGTCGTGGATAAGTTGTTT pLKO.1 1049 CDS 100% 5.625 7.875 N Afg1l n/a
2 TRCN0000336180 GCCGTCGTGGATAAGTTGTTT pLKO_005 1049 CDS 100% 5.625 7.875 N Afg1l n/a
3 TRCN0000121016 CGACGCCATGATTCTGAAACA pLKO.1 799 CDS 100% 4.950 6.930 N Afg1l n/a
4 TRCN0000121012 CCTTTGTAGATCCGAGTTTAT pLKO.1 1765 3UTR 100% 13.200 9.240 N Afg1l n/a
5 TRCN0000336124 CCTTTGTAGATCCGAGTTTAT pLKO_005 1765 3UTR 100% 13.200 9.240 N Afg1l n/a
6 TRCN0000336182 TGACTTCAAGGTGCGCATTAT pLKO_005 1330 CDS 100% 13.200 9.240 N Afg1l n/a
7 TRCN0000121015 CGGGATTCATGGCTAAGTCAT pLKO.1 693 CDS 100% 4.950 3.465 N Afg1l n/a
8 TRCN0000336122 CGGGATTCATGGCTAAGTCAT pLKO_005 693 CDS 100% 4.950 3.465 N Afg1l n/a
9 TRCN0000121013 GCCATGATTCTGAAACAGCTT pLKO.1 803 CDS 100% 2.640 1.848 N Afg1l n/a
10 TRCN0000336179 GCCATGATTCTGAAACAGCTT pLKO_005 803 CDS 100% 2.640 1.848 N Afg1l n/a
11 TRCN0000144670 GAGGACCTTAAAGGATACAAT pLKO.1 504 5UTR 100% 5.625 3.375 N AFG1L n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006512697.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05284 pDONR223 100% 62.7% 63.8% None (many diffs) n/a
2 ccsbBroad304_05284 pLX_304 0% 62.7% 63.8% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000474324 AAAGAAAACTCAAGTACTTATAAG pLX_317 38.7% 62.7% 63.8% V5 (not translated due to frame shift) (many diffs) n/a
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