Transcript: Mouse XM_006513068.3

PREDICTED: Mus musculus LIM and senescent cell antigen-like domains 1 (Lims1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lims1 (110829)
Length:
4665
CDS:
192..1388

Additional Resources:

NCBI RefSeq record:
XM_006513068.3
NBCI Gene record:
Lims1 (110829)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006513068.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000071465 GAGAAGATCGTGAACAGTAAT pLKO.1 438 CDS 100% 13.200 18.480 N Lims1 n/a
2 TRCN0000301713 GAGAAGATCGTGAACAGTAAT pLKO_005 438 CDS 100% 13.200 18.480 N Lims1 n/a
3 TRCN0000365264 TTGACATGAAGCCAGTCTGTA pLKO_005 1447 3UTR 100% 4.950 3.465 N LIMS1 n/a
4 TRCN0000071464 GCATCGCCATTATGAGAGGAA pLKO.1 1061 CDS 100% 2.640 1.848 N Lims1 n/a
5 TRCN0000301641 GCATCGCCATTATGAGAGGAA pLKO_005 1061 CDS 100% 2.640 1.848 N Lims1 n/a
6 TRCN0000071463 GCCAGTCTGTAAGAAGTGCTA pLKO.1 1457 3UTR 100% 2.640 1.848 N Lims1 n/a
7 TRCN0000301715 GCCAGTCTGTAAGAAGTGCTA pLKO_005 1457 3UTR 100% 2.640 1.848 N Lims1 n/a
8 TRCN0000071467 CCACCAATGTGGTGAATTCAT pLKO.1 590 CDS 100% 0.000 0.000 N Lims1 n/a
9 TRCN0000301643 CCACCAATGTGGTGAATTCAT pLKO_005 590 CDS 100% 0.000 0.000 N Lims1 n/a
10 TRCN0000059039 CCAGTCTGTAAGAAGTGCTAT pLKO.1 1458 3UTR 100% 4.950 3.465 N LIMS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006513068.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06524 pDONR223 100% 71.1% 76.8% None (many diffs) n/a
2 ccsbBroad304_06524 pLX_304 0% 71.1% 76.8% V5 (many diffs) n/a
3 TRCN0000468941 TAGATCCTATTATCCCACTCTTGT pLX_317 39.8% 71.1% 76.8% V5 (many diffs) n/a
4 ccsbBroadEn_04624 pDONR223 100% 25.8% 26.3% None (many diffs) n/a
5 ccsbBroad304_04624 pLX_304 0% 25.8% 26.3% V5 (many diffs) n/a
6 TRCN0000469496 CCTGCACGGGACAGCCCGTCTAAT pLX_317 100% 25.8% 26.3% V5 (many diffs) n/a
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