Construct: ORF TRCN0000469496
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006800.1_s317c1
- Derived from:
- ccsbBroadEn_04624
- DNA Barcode:
- CCTGCACGGGACAGCCCGTCTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIMS3 (96626)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469496
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100288695 | LIMS4 | LIM zinc finger domain cont... | NM_001205288.2 | 100% | 100% | |
| 2 | human | 96626 | LIMS3 | LIM zinc finger domain cont... | NM_033514.5 | 100% | 100% | |
| 3 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001371499.1 | 99.4% | 99.1% | 251A>C;351A>G |
| 4 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001371500.1 | 60.2% | 48.4% | (many diffs) |
| 5 | human | 100288695 | LIMS4 | LIM zinc finger domain cont... | XM_017003104.2 | 30.1% | 29.5% | (many diffs) |
| 6 | human | 100288695 | LIMS4 | LIM zinc finger domain cont... | XM_017003105.2 | 30.1% | 29.5% | (many diffs) |
| 7 | human | 100288695 | LIMS4 | LIM zinc finger domain cont... | XM_017003103.2 | 30% | 29.4% | (many diffs) |
| 8 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001193485.3 | 29.9% | 29.1% | (many diffs) |
| 9 | human | 100288695 | LIMS4 | LIM zinc finger domain cont... | NM_001371340.1 | 29.2% | 28.6% | (many diffs) |
| 10 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001371494.1 | 29.1% | 28.3% | (many diffs) |
| 11 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001193484.2 | 19.1% | 15% | (many diffs) |
| 12 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001371496.1 | 17.7% | 14.2% | (many diffs) |
| 13 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001193483.3 | 16.1% | 13.5% | (many diffs) |
| 14 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001371495.1 | 15.7% | 13.2% | (many diffs) |
| 15 | human | 96626 | LIMS3 | LIM zinc finger domain cont... | NR_027467.3 | 15.1% | (many diffs) | |
| 16 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001371497.1 | 14.9% | 13.4% | (many diffs) |
| 17 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001193482.2 | 14.8% | 13.4% | (many diffs) |
| 18 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001193488.2 | 13.9% | 13.1% | (many diffs) |
| 19 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_001371498.1 | 13.9% | 13.1% | (many diffs) |
| 20 | human | 3987 | LIMS1 | LIM zinc finger domain cont... | NM_004987.6 | 13.9% | 13.1% | (many diffs) |
| 21 | human | 100288695 | LIMS4 | LIM zinc finger domain cont... | NR_038099.1 | 13.6% | (many diffs) | |
| 22 | human | 100271835 | LIMS3-LOC440895 | LIMS3-LOC440895 readthrough | NR_027145.2 | 10.8% | (many diffs) | |
| 23 | mouse | 110829 | Lims1 | LIM and senescent cell anti... | XM_006513074.3 | 86.7% | 89.8% | (many diffs) |
| 24 | mouse | 110829 | Lims1 | LIM and senescent cell anti... | NM_001346676.1 | 26.6% | 27.1% | (many diffs) |
| 25 | mouse | 110829 | Lims1 | LIM and senescent cell anti... | NM_001193303.1 | 26% | 26.5% | (many diffs) |
| 26 | mouse | 110829 | Lims1 | LIM and senescent cell anti... | XM_006513068.3 | 25.8% | 26.3% | (many diffs) |
| 27 | mouse | 110829 | Lims1 | LIM and senescent cell anti... | XM_006513073.2 | 12.7% | 13.1% | (many diffs) |
| 28 | mouse | 110829 | Lims1 | LIM and senescent cell anti... | XM_006513072.2 | 12.3% | 12.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 420
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCACCAT GGCCTTCTCA GGCCGAGCGC GCCCCTGCAT TATCCCAGAG AACGAAGAAA 121 TCCCCCGAGC AGCCCTTAAC ACTGTCCACG AGGCCAATGG GACCGAGGAC GAGAGGGCTG 181 TTTCCAAACT GCAGCGCAGG CACAGTGACG TGAAAGTCTA CAAGGAGTTC TGTGACTTTT 241 ATGCGAAATT CAACATGGCC AACGCCCTGG CCAGCGCCAC TTGCGAGCGC TGCAAGGGCG 301 GCTTTGCGCC CGCTGAGACG ATCGTGAACA GTAATGGGGA GCTGTACCAT GAGCAGTGTT 361 TCGTGTGCGC TCAGTGCTTC CAGCAGTTCC CAGAAGGACT CTTCTATGAG GAACGAACGT 421 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 481 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 541 TTTATATATC TTGTGGAAAG GACGACCTGC ACGGGACAGC CCGTCTAATA CGCGTTAAGT 601 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt