Transcript: Mouse XM_006513073.2

PREDICTED: Mus musculus LIM and senescent cell antigen-like domains 1 (Lims1), transcript variant X6, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lims1 (110829)
Length:
5254
CDS:
1156..2133

Additional Resources:

NCBI RefSeq record:
XM_006513073.2
NBCI Gene record:
Lims1 (110829)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006513073.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000071465 GAGAAGATCGTGAACAGTAAT pLKO.1 1216 CDS 100% 13.200 18.480 N Lims1 n/a
2 TRCN0000301713 GAGAAGATCGTGAACAGTAAT pLKO_005 1216 CDS 100% 13.200 18.480 N Lims1 n/a
3 TRCN0000365264 TTGACATGAAGCCAGTCTGTA pLKO_005 2036 CDS 100% 4.950 3.465 N LIMS1 n/a
4 TRCN0000071464 GCATCGCCATTATGAGAGGAA pLKO.1 1839 CDS 100% 2.640 1.848 N Lims1 n/a
5 TRCN0000301641 GCATCGCCATTATGAGAGGAA pLKO_005 1839 CDS 100% 2.640 1.848 N Lims1 n/a
6 TRCN0000071463 GCCAGTCTGTAAGAAGTGCTA pLKO.1 2046 CDS 100% 2.640 1.848 N Lims1 n/a
7 TRCN0000301715 GCCAGTCTGTAAGAAGTGCTA pLKO_005 2046 CDS 100% 2.640 1.848 N Lims1 n/a
8 TRCN0000071467 CCACCAATGTGGTGAATTCAT pLKO.1 1368 CDS 100% 0.000 0.000 N Lims1 n/a
9 TRCN0000301643 CCACCAATGTGGTGAATTCAT pLKO_005 1368 CDS 100% 0.000 0.000 N Lims1 n/a
10 TRCN0000059039 CCAGTCTGTAAGAAGTGCTAT pLKO.1 2047 CDS 100% 4.950 3.465 N LIMS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006513073.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06524 pDONR223 100% 90.4% 98.7% None (many diffs) n/a
2 ccsbBroad304_06524 pLX_304 0% 90.4% 98.7% V5 (many diffs) n/a
3 TRCN0000468941 TAGATCCTATTATCCCACTCTTGT pLX_317 39.8% 90.4% 98.7% V5 (many diffs) n/a
4 ccsbBroadEn_04624 pDONR223 100% 12.7% 13.1% None (many diffs) n/a
5 ccsbBroad304_04624 pLX_304 0% 12.7% 13.1% V5 (many diffs) n/a
6 TRCN0000469496 CCTGCACGGGACAGCCCGTCTAAT pLX_317 100% 12.7% 13.1% V5 (many diffs) n/a
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