Transcript: Mouse XM_006514250.2

PREDICTED: Mus musculus DnaJ heat shock protein family (Hsp40) member C14 (Dnajc14), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dnajc14 (74330)
Length:
3078
CDS:
225..2336

Additional Resources:

NCBI RefSeq record:
XM_006514250.2
NBCI Gene record:
Dnajc14 (74330)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006514250.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000329467 CAGCAGCTGGTAAGGTTAATA pLKO_005 1389 CDS 100% 15.000 12.000 N Dnajc14 n/a
2 TRCN0000112221 CGGAAGGAATATGAGATGAAA pLKO.1 1725 CDS 100% 5.625 4.500 N Dnajc14 n/a
3 TRCN0000112224 GCCCGCATGATGTTCCAGTTT pLKO.1 1131 CDS 100% 4.950 3.960 N Dnajc14 n/a
4 TRCN0000329466 ATGATGAGGAACCTCTTAAAT pLKO_005 742 CDS 100% 15.000 10.500 N Dnajc14 n/a
5 TRCN0000329400 TGAACTAAAGAAGGCCTATAG pLKO_005 1598 CDS 100% 10.800 7.560 N Dnajc14 n/a
6 TRCN0000112220 GCCAGGAGATTATCTTTCTAT pLKO.1 3037 3UTR 100% 5.625 3.938 N Dnajc14 n/a
7 TRCN0000329396 GCCAGGAGATTATCTTTCTAT pLKO_005 3037 3UTR 100% 5.625 3.938 N Dnajc14 n/a
8 TRCN0000112223 CCTCTTAAATTTCCCAGTGAT pLKO.1 753 CDS 100% 4.950 3.465 N Dnajc14 n/a
9 TRCN0000112222 GCGGAAGGAATATGAGATGAA pLKO.1 1724 CDS 100% 4.950 3.465 N Dnajc14 n/a
10 TRCN0000329395 GCGGAAGGAATATGAGATGAA pLKO_005 1724 CDS 100% 4.950 3.465 N Dnajc14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006514250.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04482 pDONR223 100% 86.4% 85.5% None (many diffs) n/a
2 ccsbBroad304_04482 pLX_304 0% 86.4% 85.5% V5 (many diffs) n/a
3 TRCN0000468353 CTGTACCCTCCTGGTTACTTTCAC pLX_317 16.7% 86.4% 85.5% V5 (many diffs) n/a
4 ccsbBroadEn_16052 pDONR223 0% 86.3% 85.5% None (many diffs) n/a
5 ccsbBroad304_16052 pLX_304 0% 86.3% 85.5% V5 (many diffs) n/a
6 TRCN0000479570 ACTAATTGCAGCGCTCCTGTCCAG pLX_317 12.8% 86.3% 85.5% V5 (many diffs) n/a
7 ccsbBroadEn_12810 pDONR223 100% 19.6% 19.9% None (many diffs) n/a
8 ccsbBroad304_12810 pLX_304 0% 19.6% 19.9% V5 (many diffs) n/a
9 TRCN0000471746 TTGAATTCCGTTTATATTACAGAT pLX_317 39.8% 19.6% 19.9% V5 (many diffs) n/a
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