Transcript: Mouse XM_006515067.2

PREDICTED: Mus musculus diacylglycerol kinase, beta (Dgkb), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dgkb (217480)
Length:
6653
CDS:
693..3104

Additional Resources:

NCBI RefSeq record:
XM_006515067.2
NBCI Gene record:
Dgkb (217480)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148085 TGAACATGCTGATTGGCGTG pXPR_003 GGG 798 33% 10 -0.0142 Dgkb DGKB 76917
2 BRDN0001145601 GAAAGACTGTAAACCTGACG pXPR_003 AGG 1395 58% 17 -0.2252 Dgkb DGKB 76916
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006515067.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000362006 GACCCTTGAAGGACCATATTT pLKO_005 1771 CDS 100% 15.000 21.000 N Dgkb n/a
2 TRCN0000024931 CCTACCACAATCTGTCCAGTT pLKO.1 1797 CDS 100% 4.050 5.670 N Dgkb n/a
3 TRCN0000024932 CCCATTCTAGCCCAAATGTAA pLKO.1 967 CDS 100% 5.625 4.500 N Dgkb n/a
4 TRCN0000362001 GAAGCAAGGAGAACGAATTTA pLKO_005 2036 CDS 100% 15.000 10.500 N Dgkb n/a
5 TRCN0000362002 CAGACGTCATGCACCACTATT pLKO_005 1603 CDS 100% 13.200 9.240 N Dgkb n/a
6 TRCN0000024930 CCCTCCTGCATCAAGACATAT pLKO.1 1563 CDS 100% 13.200 9.240 N Dgkb n/a
7 TRCN0000024860 GCAATGGAAATGGGCCAAATT pLKO.1 2856 CDS 100% 13.200 9.240 N LOC382588 n/a
8 TRCN0000025549 GCTGCATGAATCTGTAGAAAT pLKO.1 2594 CDS 100% 13.200 9.240 N LOC382587 n/a
9 TRCN0000024929 CGAGTTTATGTTTCGCCTTTA pLKO.1 1151 CDS 100% 10.800 7.560 N Dgkb n/a
10 TRCN0000024859 CCTCTGTGGTTATCAGAACAA pLKO.1 2920 CDS 100% 4.950 3.465 N LOC382588 n/a
11 TRCN0000024933 GCTACCTGTCTCTTCTCGAAA pLKO.1 1108 CDS 100% 4.950 3.465 N Dgkb n/a
12 TRCN0000024863 GCTTGGAAGGAGCAATGGAAA pLKO.1 2845 CDS 100% 4.950 3.465 N LOC382588 n/a
13 TRCN0000024861 CCAAATTTACACGGGCCTGAA pLKO.1 2870 CDS 100% 4.050 2.835 N LOC382588 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006515067.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14607 pDONR223 68.3% 88.2% 28.3% None (many diffs) n/a
2 ccsbBroad304_14607 pLX_304 0% 88.2% 28.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000473795 GATAATAGTCGCCATTAGCGACGG pLX_317 16.3% 88.2% 28.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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