Construct: ORF ccsbBroadEn_14607
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018913.1_s300c1, BRDN0000458209
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DGKB (1607)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350715.1 | 98.9% | 29.2% | (many diffs) |
| 2 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350716.2 | 98.9% | 29.2% | (many diffs) |
| 3 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350711.2 | 98.7% | 29.2% | (many diffs) |
| 4 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350712.1 | 98.7% | 29.2% | (many diffs) |
| 5 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350714.2 | 98.7% | 29.2% | (many diffs) |
| 6 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350705.1 | 98% | 28.9% | (many diffs) |
| 7 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350706.2 | 98% | 28.9% | (many diffs) |
| 8 | human | 1607 | DGKB | diacylglycerol kinase beta | XM_011515154.2 | 98% | 28.9% | (many diffs) |
| 9 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350707.2 | 97.9% | 29% | (many diffs) |
| 10 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350708.1 | 97.9% | 29% | (many diffs) |
| 11 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350709.2 | 97.9% | 29% | (many diffs) |
| 12 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350720.1 | 97.4% | 29.6% | (many diffs) |
| 13 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350721.2 | 97.4% | 29.6% | (many diffs) |
| 14 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350717.2 | 96.5% | 29.4% | (many diffs) |
| 15 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350718.1 | 96.5% | 29.4% | (many diffs) |
| 16 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350719.2 | 96.5% | 29.4% | (many diffs) |
| 17 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350722.2 | 94% | 23.5% | (many diffs) |
| 18 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350723.2 | 94% | 23.5% | (many diffs) |
| 19 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_145695.2 | 93.9% | 30.1% | (many diffs) |
| 20 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350724.2 | 93.5% | 23.3% | (many diffs) |
| 21 | human | 1607 | DGKB | diacylglycerol kinase beta | XM_017011790.1 | 87.6% | 32.1% | (many diffs) |
| 22 | human | 1607 | DGKB | diacylglycerol kinase beta | XM_017011791.1 | 74.5% | 37.5% | (many diffs) |
| 23 | human | 1607 | DGKB | diacylglycerol kinase beta | XM_017011792.2 | 74.4% | 37.8% | (many diffs) |
| 24 | human | 1607 | DGKB | diacylglycerol kinase beta | XR_001744571.1 | 48.7% | (many diffs) | |
| 25 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515070.2 | 89% | 28.6% | (many diffs) |
| 26 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515071.2 | 89% | 28.6% | (many diffs) |
| 27 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | NM_178681.4 | 88.9% | 28.6% | (many diffs) |
| 28 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515072.3 | 88.9% | 28.6% | (many diffs) |
| 29 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515067.2 | 88.2% | 28.3% | (many diffs) |
| 30 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515068.2 | 88.2% | 28.3% | (many diffs) |
| 31 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515069.2 | 88.1% | 28.3% | (many diffs) |
| 32 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_017315019.1 | 88.1% | 28.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 800
- ORF length:
- 702
- Sequence:
-
1 TACGCTTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG ACAAACCAGG AAAAATGGGC 121 CCACCTCAGC CCTTCGGAAT TTTCCCAACT TCAGAAATAT GCTGAGTATT CTACAAAGAA 181 ATTAAAGGAT GTTCTTGAAG AATTCCATGG TAATGGTGTG CTTGCAAAGT ATAATCCTGA 241 AGGGACAATA GATTTTGAAG GTTTCAAACT ATTCATGAAG ACATTCCTGG AAGCCGAGCT 301 TCCTGATGAT TTCACTGCAC ACCTTTTCAT GTCATTTAGC AACAAGTTTC CTCATTCTAG 361 TCCAATGGTA AAAAGTAAGC CTGCTCTCCT ATCAGGCGGT CTGAGAATGA ATAAAGGTGC 421 CATCACCCCT CCCCGAACTA CTTCTCCTGC AAATACGTGT TCCCCAGAAG TAATCCATCT 481 GAAGGACATT GTCTGTTACC TGTCTCTGCT TGAAAGAGGA AGACCTGAGG ATAAGCTTGA 541 GTTTATGTTT CGCCTTTATG ACACGGATGG GAATGGCTTC CTGGACAGCT CGGAGCTAGA 601 AAATATCATC AGTCAGATGA TGCATGTTGC AGAATACCTT GAGTGGGATG TCACTGAACT 661 TAATCCAATC CTCCATGAAA TGATGGAAGA AATTGACTAT GATCATGATG GAACCGTGTC 721 TCTGGAGGAA TGGATTCAAG GAGGAATGAC AACGATTCCA CTTCTTGTGC TCCTGGGCTT 781 AGAAAATAAC GTGAAAGGAT GATGGACAGC ACGTGTGGCG ACTGAGCACT TTAACAAACC 841 TGCTATGCAC TTTGCTGACA TGCTGATTGC GTGGGGAAGC AGGCTCTGCT GTTCCTTCTG 901 CAGTACACAG TCCATGAgcg ctgtgtggct cgagcacctc cctcttgcat caagacctat 961 gtgaagtcca aaaggaacac tgatgtcatg caccattact gggttgaagg taactgccca 1021 accaagtgtg ataagtgcca caaaactgtt aaatgttacc agggcctgac aggactgcat 1081 tgtgtttggt gtcagatcac actgcataat aaatgtgctt ctcatctaaa acctgaatgt 1141 gactgtggac ctttgaagga ccatatttta ccacccacaa caatctgtcc agtggtactg 1201 cagactctgc ccacttcagg agtttcagtt cctgaggaaa gacaatcaac agtgaaaaag 1261 gaaaagagtg gttcccagca gccaaacaaa gtgattgaca agaataaaat gcaaagagcc 1321 aactctgtta ctgtagatgg acaaggcctg caggtcactc ctgtgcctgg tactcaccca 1381 cttttagttt ttgtgaaccc caaaagtggt ggaaaacaag gagaacgaat ttacagaaaa 1441 ttccagtatc tattaaatcc tcgtcaggtt tacagtcttt ctggaaatgg accaatgcca 1501 gggttaaact ttttccgtga tgttcctgac ttcagagtgt tagcctgtgg tggagatgga 1561 accgtgggct gggttttgga ttgcatagaa aaggccaatg taggcaagca tcctccagtt 1621 gcgattctgc ctcttgggac tggcaatgat ctagcaagat gcctgcgatg gggaggaggT 1681 TACGAAGGTG AGGATCTGAT GAAAATTTTA AAAGACATTG NAAACAGCAC AGAAATCATG 1741 TGGACAGGTG GAAGTTTGAA AGTCATACNT AATGACAAGN ATGAGAAAGG AGACCCAGTG 1801 CCTANCAGTA TCATCAATAA TTACTTTTCC ATTGGCGTGG ATGCCTCCAT GNCACACAGA 1861 TTCCACATCA TGAGAGAAAA ACACCCNAGA GAAATTCAAC AGTAGAATGA AGAACAAATT 1921 TTGGTATTTT GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA 1981 ATCTGTAGAA ATAGAATGTG ATGGAGTACA GATAGATTTA ATAAACATCT CTCTGGAAGG 2041 AATTGCTATT TTGAATATAC CAAGCATGCA TGGAGGATCC AATCTTTGGG GAGAGTCTAA 2101 GAAAAGACGA AGCCATCGAC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACCGTCAC 2161 AGATGCCAAA GAGTTGAAGT TTGCAAGTCA AGATCTCAGT GACCAGCTGC TGGAGGTGGT 2221 CGGCTTGGAA GGAGCCATGG AGATGGGGCA AATATACACA GGCCTGAAAA GTGCTGGCCG 2281 GCGGCTGGCT CAGTGCTCCT GCGTGGTCAT CAGGACGAGC AAGTCTCTGC CAATGCAAAT 2341 TGATGGGGAG CCATGGATGC AGACCCCATG CACAATAAAA ATTACACACA AGAACCAAGC 2401 CCCAATGCTG ATGGGCCCGC CTCCAAAAAC CGGTTTATTC TGCTCCCTCG TCAAAAGGAC 2461 AAGAAACCGA AGCAAGGAAT TGCCAACTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2521 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTGC 2581 CATCCCAGCT GAAATC