Construct: ORF ccsbBroad304_14607
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018913.1_s304c1, BRDN0000459397
- Derived from:
- ccsbBroadEn_14607
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DGKB (1607)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14607
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350715.1 | 98.9% | 29.2% | (many diffs) |
| 2 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350716.2 | 98.9% | 29.2% | (many diffs) |
| 3 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350711.2 | 98.7% | 29.2% | (many diffs) |
| 4 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350712.1 | 98.7% | 29.2% | (many diffs) |
| 5 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350714.2 | 98.7% | 29.2% | (many diffs) |
| 6 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350705.1 | 98% | 28.9% | (many diffs) |
| 7 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350706.2 | 98% | 28.9% | (many diffs) |
| 8 | human | 1607 | DGKB | diacylglycerol kinase beta | XM_011515154.2 | 98% | 28.9% | (many diffs) |
| 9 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350707.2 | 97.9% | 29% | (many diffs) |
| 10 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350708.1 | 97.9% | 29% | (many diffs) |
| 11 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350709.2 | 97.9% | 29% | (many diffs) |
| 12 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350720.1 | 97.4% | 29.6% | (many diffs) |
| 13 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350721.2 | 97.4% | 29.6% | (many diffs) |
| 14 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350717.2 | 96.5% | 29.4% | (many diffs) |
| 15 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350718.1 | 96.5% | 29.4% | (many diffs) |
| 16 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350719.2 | 96.5% | 29.4% | (many diffs) |
| 17 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350722.2 | 94% | 23.5% | (many diffs) |
| 18 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350723.2 | 94% | 23.5% | (many diffs) |
| 19 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_145695.2 | 93.9% | 30.1% | (many diffs) |
| 20 | human | 1607 | DGKB | diacylglycerol kinase beta | NM_001350724.2 | 93.5% | 23.3% | (many diffs) |
| 21 | human | 1607 | DGKB | diacylglycerol kinase beta | XM_017011790.1 | 87.6% | 32.1% | (many diffs) |
| 22 | human | 1607 | DGKB | diacylglycerol kinase beta | XM_017011791.1 | 74.5% | 37.5% | (many diffs) |
| 23 | human | 1607 | DGKB | diacylglycerol kinase beta | XM_017011792.2 | 74.4% | 37.8% | (many diffs) |
| 24 | human | 1607 | DGKB | diacylglycerol kinase beta | XR_001744571.1 | 48.7% | (many diffs) | |
| 25 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515070.2 | 89% | 28.6% | (many diffs) |
| 26 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515071.2 | 89% | 28.6% | (many diffs) |
| 27 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | NM_178681.4 | 88.9% | 28.6% | (many diffs) |
| 28 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515072.3 | 88.9% | 28.6% | (many diffs) |
| 29 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515067.2 | 88.2% | 28.3% | (many diffs) |
| 30 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515068.2 | 88.2% | 28.3% | (many diffs) |
| 31 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_006515069.2 | 88.1% | 28.3% | (many diffs) |
| 32 | mouse | 217480 | Dgkb | diacylglycerol kinase, beta | XM_017315019.1 | 88.1% | 28.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 771
- ORF length:
- 702
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gacaaaccag gaaaaatggg cccacctcag cccttcggaa ttttcccaac 121 ttcagaaata tgctgagtat tctacaaaga aattaaagga tgttcttgaa gaattccatg 181 gtaatggtgt gcttgcaaag tataatcctg aagggacaat agattttgaa ggtttcaaac 241 tattcatgaa gacattcctg gaagccgagc ttcctgatga tttcactgca caccttttca 301 tgtcatttag caacaagttt cctcattcta gtccaatggt aaaaagtaag cctgctctcc 361 tatcaggcgg tctgagaatg aataaaggtg ccatcacccc tccccgaact acttctcctg 421 caaatacgtg ttccccagaa gtaatccatc tgaaggacat tgtctgttac ctgtctctgc 481 ttgaaagagg aagacctgag gataagcttg agtttatgtt tcgcctttat gacacggatg 541 ggaatggctt cctggacagc tcggagctag aaaatatcat cagtcagatg atgcatgttg 601 cagaatacct tgagtgggat gtcactgaac ttaatccaat cctccatgaa atgatggaag 661 aaattgacta tgatcatgat ggaaccgtgt ctctggagga atggattcaa ggaggaatga 721 caacgattcc acttcttgtg ctcctgggct tagaaaataa cgtgaaagga tgatggacag 781 cacgtgtggc gactgagcac tttaacaaac ctgctatgca ctttgctgac atgctgattg 841 cgtggggaag caggctctgc tgttccttct gcagtacaca gtccatgagc gctgtgtggc 901 tcgagcacct ccctcttgca tcaagaccta tgtgaagtcc aaaaggaaca ctgatgtcat 961 gcaccattac tgggttgaag gtaactgccc aaccaagtgt gataagtgcc acaaaactgt 1021 taaatgttac cagggcctga caggactgca ttgtgtttgg tgtcagatca cactgcataa 1081 taaatgtgct tctcatctaa aacctgaatg tgactgtgga cctttgaagg accatatttt 1141 accacccaca acaatctgtc cagtggtact gcagactctg cccacttcag gagtttcagt 1201 tcctgaggaa agacaatcaa cagtgaaaaa ggaaaagagt ggttcccagc agccaaacaa 1261 agtgattgac aagaataaaa tgcaaagagc caactctgtt actgtagatg gacaaggcct 1321 gcaggtcact cctgtgcctg gtactcaccc acttttagtt tttgtgaacc ccaaaagtgg 1381 tggaaaacaa ggagaacgaa tttacagaaa attccagtat ctattaaatc ctcgtcaggt 1441 ttacagtctt tctggaaatg gaccaatgcc agggttaaac tttttccgtg atgttcctga 1501 cttcagagtg ttagcctgtg gtggagatgg aaccgtgggc tgggttttgg attgcataga 1561 aaaggccaat gtaggcaagc atcctccagt tgcgattctg cctcttggga ctggcaatga 1621 tctagcaaga tgcctgcgat ggggaggagg ttacgaaggt gaggatctga tgaaaatttt 1681 aaaagacatt gnaaacagca cagaaatcat gtggacaggt ggaagtttga aagtcatacn 1741 taatgacaag natgagaaag gagacccagt gcctancagt atcatcaata attacttttc 1801 cattggcgtg gatgcctcca tgncacacag attccacatc atgagagaaa aacacccnag 1861 agaaattcaa cagtagaatg aagaacaaat tttggtattt tgagtttggc acatctgaaa 1921 ctttctcagc cacctgcaag aagctacatg aatctgtaga aatagaatgt gatggagtac 1981 agatagattt aataaacatc tctctggaag gaattgctat tttgaatata ccaagcatgc 2041 atggaggatc caatctttgg ggagagtcta agaaaagacg aagccatcga cgaatagaga 2101 aaaaagggtc tgacaaaagg accaccgtca cagatgccaa agagttgaag tttgcaagtc 2161 aagatctcag tgaccagctg ctggaggtgg tcggcttgga aggagccatg gagatggggc 2221 aaatatacac aggcctgaaa agtgctggcc ggcggctggc tcagtgctcc tgcgtggtca 2281 tcaggacgag caagtctctg ccaatgcaaa ttgatgggga gccatggatg cagaccccat 2341 gcacaataaa aattacacac aagaaccaag ccccaatgct gatgggcccg cctccaaaaa 2401 ccggtttatt ctgctccctc gtcaaaagga caagaaaccg aagcaaggaa ttgccaactt 2461 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 2521 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta