Transcript: Mouse XM_006518449.3

PREDICTED: Mus musculus adrenergic receptor, alpha 1a (Adra1a), transcript variant X8, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Adra1a (11549)
Length:
10819
CDS:
1182..2318

Additional Resources:

NCBI RefSeq record:
XM_006518449.3
NBCI Gene record:
Adra1a (11549)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006518449.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000219382 ACGCTCCGTATCCACCGTAAA pLKO.1 1878 CDS 100% 10.800 15.120 N Adra1a n/a
2 TRCN0000026306 CCTTTCAGAATGTGCTGCGAA pLKO.1 2188 CDS 100% 2.640 3.696 N Adra1a n/a
3 TRCN0000219381 GCTAGGATTTCAGCATATAAT pLKO.1 2505 3UTR 100% 15.000 10.500 N Adra1a n/a
4 TRCN0000219385 AGTAAGCAGTGCCAAGAATAA pLKO.1 1922 CDS 100% 13.200 9.240 N Adra1a n/a
5 TRCN0000219384 CGCCAGCACAGGTGAACATTT pLKO.1 1231 CDS 100% 13.200 9.240 N Adra1a n/a
6 TRCN0000026282 GCCCTGAAACTGGAGGTTTAT pLKO.1 2774 3UTR 100% 13.200 9.240 N Adra1a n/a
7 TRCN0000219380 GTTGCATCAACCCTATCATAT pLKO.1 2137 CDS 100% 13.200 9.240 N Adra1a n/a
8 TRCN0000219387 GGATGAGACCATCTGCCAAAT pLKO.1 1694 CDS 100% 10.800 7.560 N Adra1a n/a
9 TRCN0000219386 TGCCCTTCTCTGCCATCTTTG pLKO.1 1420 CDS 100% 10.800 7.560 N Adra1a n/a
10 TRCN0000219388 TCGGTGACTCACTACTACATT pLKO.1 1356 CDS 100% 5.625 3.938 N Adra1a n/a
11 TRCN0000026238 CGAATTTCAAGCCACCGGAAA pLKO.1 2077 CDS 100% 4.050 2.835 N Adra1a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006518449.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488275 GGCTAGGCGGACTGTCGTCCAAGA pLX_317 18.2% 70.4% 75.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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