Construct: ORF TRCN0000488275

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020980.1_s317c1
DNA Barcode:
GGCTAGGCGGACTGTCGTCCAAGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ADRA1A (148)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488275
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 148 ADRA1A adrenoceptor alpha 1A NM_000680.4 99.9% 99.7% 1039T>C
2 human 148 ADRA1A adrenoceptor alpha 1A NM_033304.3 93.3% 87.2% (many diffs)
3 human 148 ADRA1A adrenoceptor alpha 1A NM_033302.3 91.7% 90.5% (many diffs)
4 human 148 ADRA1A adrenoceptor alpha 1A XM_006716293.4 91.7% 90.5% (many diffs)
5 human 148 ADRA1A adrenoceptor alpha 1A NM_033303.4 91.4% 88.6% (many diffs)
6 human 148 ADRA1A adrenoceptor alpha 1A XM_006716292.3 91.4% 88.6% (many diffs)
7 human 148 ADRA1A adrenoceptor alpha 1A XM_017013094.1 91.4% 88.6% (many diffs)
8 human 148 ADRA1A adrenoceptor alpha 1A XM_017013095.1 91.4% 88.6% (many diffs)
9 human 148 ADRA1A adrenoceptor alpha 1A NM_001322502.1 72.7% 65.8% (many diffs)
10 human 148 ADRA1A adrenoceptor alpha 1A NM_001322503.1 72.7% 58.6% (many diffs)
11 human 148 ADRA1A adrenoceptor alpha 1A NM_001322504.1 69.3% 66.7% (many diffs)
12 human 148 ADRA1A adrenoceptor alpha 1A XM_011544411.2 66.2% 64.5% (many diffs)
13 human 148 ADRA1A adrenoceptor alpha 1A XM_017013096.1 66% 62.9% (many diffs)
14 human 148 ADRA1A adrenoceptor alpha 1A XM_011544412.3 65% 62.9% (many diffs)
15 human 148 ADRA1A adrenoceptor alpha 1A NR_136343.1 48.6% (many diffs)
16 human 148 ADRA1A adrenoceptor alpha 1A XR_001745476.1 42.8% (many diffs)
17 human 148 ADRA1A adrenoceptor alpha 1A XR_001745477.1 36.9% 1_1021del;1904_2088del;2168_2169ins436
18 mouse 11549 Adra1a adrenergic receptor, alpha 1a NM_001271760.1 87.2% 92% (many diffs)
19 mouse 11549 Adra1a adrenergic receptor, alpha 1a NM_013461.4 87.2% 92% (many diffs)
20 mouse 11549 Adra1a adrenergic receptor, alpha 1a XM_006518444.3 87.2% 92% (many diffs)
21 mouse 11549 Adra1a adrenergic receptor, alpha 1a XM_011244923.2 87.2% 92% (many diffs)
22 mouse 11549 Adra1a adrenergic receptor, alpha 1a XM_011244924.2 87.2% 92% (many diffs)
23 mouse 11549 Adra1a adrenergic receptor, alpha 1a XM_011244925.2 87.2% 92% (many diffs)
24 mouse 11549 Adra1a adrenergic receptor, alpha 1a XM_017315800.1 87.2% 92% (many diffs)
25 mouse 11549 Adra1a adrenergic receptor, alpha 1a XM_017315801.1 87.2% 92% (many diffs)
26 mouse 11549 Adra1a adrenergic receptor, alpha 1a XM_011244926.2 81.6% 86.2% (many diffs)
27 mouse 11549 Adra1a adrenergic receptor, alpha 1a NM_001271759.1 79.7% 84.5% (many diffs)
28 mouse 11549 Adra1a adrenergic receptor, alpha 1a XM_006518449.3 70.4% 75.5% (many diffs)
29 mouse 11549 Adra1a adrenergic receptor, alpha 1a NM_001271761.1 55.2% 59.2% (many diffs)
30 mouse 11549 Adra1a adrenergic receptor, alpha 1a XM_017315802.1 55.2% 59.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1470
ORF length:
1398
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggtgttt ctctcgggaa atgcttccga cagctccaac tgcacccaac
121cgccggcacc ggtgaacatt tccaaggcca ttctgctcgg ggtgatcttg gggggcctca
181ttcttttcgg ggtgctgggt aacatcctag tgatcctctc cgtagcctgt caccgacacc
241tgcactcagt cacgcactac tacatcgtca acctggcggt ggccgacctc ctgctcacct
301ccacggtgct gcccttctcc gccatcttcg aggtcctagg ctactgggcc ttcggcaggg
361tcttctgcaa catctgggcg gcagtggatg tgctgtgctg caccgcgtcc atcatgggcc
421tctgcatcat ctccatcgac cgctacatcg gcgtgagcta cccgctgcgc tacccaacca
481tcgtcaccca gaggaggggt ctcatggctc tgctctgcgt ctgggcactc tccctggtca
541tatccattgg acccctgttc ggctggaggc agccggcccc cgaggacgag accatctgcc
601agatcaacga ggagccgggc tacgtgctct tctcagcgct gggctccttc tacctgcctc
661tggccatcat cctggtcatg tactgccgcg tctacgtggt ggccaagagg gagagccggg
721gcctcaagtc tggcctcaag accgacaagt cggactcgga gcaagtgacg ctccgcatcc
781atcggaaaaa cgccccggca ggaggcagcg ggatggccag cgccaagacc aagacgcact
841tctcagtgag gctcctcaag ttctcccggg agaagaaagc ggccaaaacg ctgggcatcg
901tggtcggctg cttcgtcctc tgctggctgc cttttttctt agtcatgccc attgggtctt
961tcttccctga tttcaagccc tctgaaacag tttttaaaat agtattttgg ctcggatatc
1021taaacagctg catcaacccc atcatatacc catgctccag ccaagagttc aaaaaggcct
1081ttcagaatgt cttgagaatc cagtgtctcc gcagaaagca gtcttccaaa catgccctgg
1141gctacaccct gcacccgccc agccaggccg tggaagggca acacaaggac atggtgcgca
1201tccccgtggg atcaagagag accTTCTACA GGATCTCCAA GACGGATGGC GTTTGTGAAT
1261GGAAATTTTT CTCTTCCATG CCCCGTGGAT CTGCCAGGAT TACAGTGTCC AAAGACCAAT
1321CCTCCTGTAC CACAGCCCGG GTGAGAAGTA AAAGCTTTTT GCAGGTCTGC TGCTGTGTAG
1381GGCCCTCAAC CCCCAGCCTT GACAAGAACC ATCAAGTTCC AACCATTAAG GTCCACACCA
1441TCTCCCTCAG TGAGAACGGG GAGGAAGTCT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG
1501ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1561GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG
1621CTAGGCGGAC TGTCGTCCAA GAACGCGTTA AGTCgacaat caacctctgg attacaaaat
1681ttgtgaaaga tt

Download FASTA (ORF) (Full)

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