Construct: ORF TRCN0000488275
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020980.1_s317c1
- DNA Barcode:
- GGCTAGGCGGACTGTCGTCCAAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADRA1A (148)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488275
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148 | ADRA1A | adrenoceptor alpha 1A | NM_000680.4 | 99.9% | 99.7% | 1039T>C |
| 2 | human | 148 | ADRA1A | adrenoceptor alpha 1A | NM_033304.3 | 93.3% | 87.2% | (many diffs) |
| 3 | human | 148 | ADRA1A | adrenoceptor alpha 1A | NM_033302.3 | 91.7% | 90.5% | (many diffs) |
| 4 | human | 148 | ADRA1A | adrenoceptor alpha 1A | XM_006716293.4 | 91.7% | 90.5% | (many diffs) |
| 5 | human | 148 | ADRA1A | adrenoceptor alpha 1A | NM_033303.4 | 91.4% | 88.6% | (many diffs) |
| 6 | human | 148 | ADRA1A | adrenoceptor alpha 1A | XM_006716292.3 | 91.4% | 88.6% | (many diffs) |
| 7 | human | 148 | ADRA1A | adrenoceptor alpha 1A | XM_017013094.1 | 91.4% | 88.6% | (many diffs) |
| 8 | human | 148 | ADRA1A | adrenoceptor alpha 1A | XM_017013095.1 | 91.4% | 88.6% | (many diffs) |
| 9 | human | 148 | ADRA1A | adrenoceptor alpha 1A | NM_001322502.1 | 72.7% | 65.8% | (many diffs) |
| 10 | human | 148 | ADRA1A | adrenoceptor alpha 1A | NM_001322503.1 | 72.7% | 58.6% | (many diffs) |
| 11 | human | 148 | ADRA1A | adrenoceptor alpha 1A | NM_001322504.1 | 69.3% | 66.7% | (many diffs) |
| 12 | human | 148 | ADRA1A | adrenoceptor alpha 1A | XM_011544411.2 | 66.2% | 64.5% | (many diffs) |
| 13 | human | 148 | ADRA1A | adrenoceptor alpha 1A | XM_017013096.1 | 66% | 62.9% | (many diffs) |
| 14 | human | 148 | ADRA1A | adrenoceptor alpha 1A | XM_011544412.3 | 65% | 62.9% | (many diffs) |
| 15 | human | 148 | ADRA1A | adrenoceptor alpha 1A | NR_136343.1 | 48.6% | (many diffs) | |
| 16 | human | 148 | ADRA1A | adrenoceptor alpha 1A | XR_001745476.1 | 42.8% | (many diffs) | |
| 17 | human | 148 | ADRA1A | adrenoceptor alpha 1A | XR_001745477.1 | 36.9% | 1_1021del;1904_2088del;2168_2169ins436 | |
| 18 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | NM_001271760.1 | 87.2% | 92% | (many diffs) |
| 19 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | NM_013461.4 | 87.2% | 92% | (many diffs) |
| 20 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | XM_006518444.3 | 87.2% | 92% | (many diffs) |
| 21 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | XM_011244923.2 | 87.2% | 92% | (many diffs) |
| 22 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | XM_011244924.2 | 87.2% | 92% | (many diffs) |
| 23 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | XM_011244925.2 | 87.2% | 92% | (many diffs) |
| 24 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | XM_017315800.1 | 87.2% | 92% | (many diffs) |
| 25 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | XM_017315801.1 | 87.2% | 92% | (many diffs) |
| 26 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | XM_011244926.2 | 81.6% | 86.2% | (many diffs) |
| 27 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | NM_001271759.1 | 79.7% | 84.5% | (many diffs) |
| 28 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | XM_006518449.3 | 70.4% | 75.5% | (many diffs) |
| 29 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | NM_001271761.1 | 55.2% | 59.2% | (many diffs) |
| 30 | mouse | 11549 | Adra1a | adrenergic receptor, alpha 1a | XM_017315802.1 | 55.2% | 59.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1470
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtgttt ctctcgggaa atgcttccga cagctccaac tgcacccaac 121 cgccggcacc ggtgaacatt tccaaggcca ttctgctcgg ggtgatcttg gggggcctca 181 ttcttttcgg ggtgctgggt aacatcctag tgatcctctc cgtagcctgt caccgacacc 241 tgcactcagt cacgcactac tacatcgtca acctggcggt ggccgacctc ctgctcacct 301 ccacggtgct gcccttctcc gccatcttcg aggtcctagg ctactgggcc ttcggcaggg 361 tcttctgcaa catctgggcg gcagtggatg tgctgtgctg caccgcgtcc atcatgggcc 421 tctgcatcat ctccatcgac cgctacatcg gcgtgagcta cccgctgcgc tacccaacca 481 tcgtcaccca gaggaggggt ctcatggctc tgctctgcgt ctgggcactc tccctggtca 541 tatccattgg acccctgttc ggctggaggc agccggcccc cgaggacgag accatctgcc 601 agatcaacga ggagccgggc tacgtgctct tctcagcgct gggctccttc tacctgcctc 661 tggccatcat cctggtcatg tactgccgcg tctacgtggt ggccaagagg gagagccggg 721 gcctcaagtc tggcctcaag accgacaagt cggactcgga gcaagtgacg ctccgcatcc 781 atcggaaaaa cgccccggca ggaggcagcg ggatggccag cgccaagacc aagacgcact 841 tctcagtgag gctcctcaag ttctcccggg agaagaaagc ggccaaaacg ctgggcatcg 901 tggtcggctg cttcgtcctc tgctggctgc cttttttctt agtcatgccc attgggtctt 961 tcttccctga tttcaagccc tctgaaacag tttttaaaat agtattttgg ctcggatatc 1021 taaacagctg catcaacccc atcatatacc catgctccag ccaagagttc aaaaaggcct 1081 ttcagaatgt cttgagaatc cagtgtctcc gcagaaagca gtcttccaaa catgccctgg 1141 gctacaccct gcacccgccc agccaggccg tggaagggca acacaaggac atggtgcgca 1201 tccccgtggg atcaagagag accTTCTACA GGATCTCCAA GACGGATGGC GTTTGTGAAT 1261 GGAAATTTTT CTCTTCCATG CCCCGTGGAT CTGCCAGGAT TACAGTGTCC AAAGACCAAT 1321 CCTCCTGTAC CACAGCCCGG GTGAGAAGTA AAAGCTTTTT GCAGGTCTGC TGCTGTGTAG 1381 GGCCCTCAAC CCCCAGCCTT GACAAGAACC ATCAAGTTCC AACCATTAAG GTCCACACCA 1441 TCTCCCTCAG TGAGAACGGG GAGGAAGTCT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 1501 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1561 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 1621 CTAGGCGGAC TGTCGTCCAA GAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1681 ttgtgaaaga tt