Construct: ORF TRCN0000478424

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007358.1_s317c1
Derived from:
ccsbBroadEn_08780
DNA Barcode:
GAGTTTAGGGGTTTCCTCCTTGGC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
ARHGAP22 (58504)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478424
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 58504 ARHGAP22 Rho GTPase activating prote... NM_021226.4 99.6% 38.6% (many diffs)
2 human 58504 ARHGAP22 Rho GTPase activating prote... NM_001256025.3 98.2% 37% (many diffs)
3 human 58504 ARHGAP22 Rho GTPase activating prote... NM_001347738.2 98.2% 37% (many diffs)
4 human 58504 ARHGAP22 Rho GTPase activating prote... NM_001256024.2 97.4% 37.8% (many diffs)
5 human 58504 ARHGAP22 Rho GTPase activating prote... XM_011540002.2 96% 36.1% (many diffs)
6 human 58504 ARHGAP22 Rho GTPase activating prote... XM_011540003.1 96% 36.1% (many diffs)
7 human 58504 ARHGAP22 Rho GTPase activating prote... XM_024448099.1 96% 36.1% (many diffs)
8 human 58504 ARHGAP22 Rho GTPase activating prote... NM_001347735.2 93.5% 32.5% (many diffs)
9 human 58504 ARHGAP22 Rho GTPase activating prote... XM_011540006.2 92.2% 30.9% (many diffs)
10 human 58504 ARHGAP22 Rho GTPase activating prote... NM_001256026.2 86.1% 23.7% (many diffs)
11 human 58504 ARHGAP22 Rho GTPase activating prote... XM_005270014.3 84.3% 23.2% (many diffs)
12 human 58504 ARHGAP22 Rho GTPase activating prote... XM_011540005.1 84.2% 23.2% (many diffs)
13 human 58504 ARHGAP22 Rho GTPase activating prote... XM_011540011.2 80% 17.9% (many diffs)
14 human 58504 ARHGAP22 Rho GTPase activating prote... XM_011540012.3 78.5% 17.3% (many diffs)
15 human 58504 ARHGAP22 Rho GTPase activating prote... XM_017016471.2 78.5% 17.3% (many diffs)
16 human 58504 ARHGAP22 Rho GTPase activating prote... NR_144645.2 74.5% (many diffs)
17 human 58504 ARHGAP22 Rho GTPase activating prote... XR_002956998.1 70.1% (many diffs)
18 human 58504 ARHGAP22 Rho GTPase activating prote... NR_144646.2 68.9% (many diffs)
19 human 58504 ARHGAP22 Rho GTPase activating prote... NR_144643.2 65.3% (many diffs)
20 human 58504 ARHGAP22 Rho GTPase activating prote... XR_002957004.1 65.2% (many diffs)
21 human 58504 ARHGAP22 Rho GTPase activating prote... XR_001747166.2 65.2% (many diffs)
22 human 58504 ARHGAP22 Rho GTPase activating prote... NR_144642.2 63.6% (many diffs)
23 human 58504 ARHGAP22 Rho GTPase activating prote... NR_144644.2 61.2% (many diffs)
24 human 58504 ARHGAP22 Rho GTPase activating prote... NR_045675.2 60.6% (many diffs)
25 human 58504 ARHGAP22 Rho GTPase activating prote... XR_002956999.1 59.2% (many diffs)
26 human 58504 ARHGAP22 Rho GTPase activating prote... XM_024448101.1 56.5% 14.6% (many diffs)
27 human 58504 ARHGAP22 Rho GTPase activating prote... XM_017016476.1 54.7% 65.2% (many diffs)
28 human 58504 ARHGAP22 Rho GTPase activating prote... XM_024448100.1 53.8% 61.9% (many diffs)
29 human 58504 ARHGAP22 Rho GTPase activating prote... XM_011540013.3 52.6% 59.7% (many diffs)
30 human 58504 ARHGAP22 Rho GTPase activating prote... XM_011540015.3 45.6% 72.5% (many diffs)
31 human 58504 ARHGAP22 Rho GTPase activating prote... XR_002957000.1 42.2% (many diffs)
32 human 58504 ARHGAP22 Rho GTPase activating prote... XR_002957003.1 39.3% (many diffs)
33 human 58504 ARHGAP22 Rho GTPase activating prote... XR_002957002.1 38.5% (many diffs)
34 human 58504 ARHGAP22 Rho GTPase activating prote... XM_024448102.1 16.8% 36.6% (many diffs)
35 human 58504 ARHGAP22 Rho GTPase activating prote... XM_024448103.1 15.9% 35.6% (many diffs)
36 human 58504 ARHGAP22 Rho GTPase activating prote... XM_024448104.1 15.8% 36.2% (many diffs)
37 human 58504 ARHGAP22 Rho GTPase activating prote... XM_024448105.1 15.4% 35.4% (many diffs)
38 human 58504 ARHGAP22 Rho GTPase activating prote... NM_001347737.1 11.5% 28.7% (many diffs)
39 human 58504 ARHGAP22 Rho GTPase activating prote... NM_001347736.1 11% 24.8% (many diffs)
40 human 58504 ARHGAP22 Rho GTPase activating prote... XM_024448106.1 11% 24.8% (many diffs)
41 mouse 239027 Arhgap22 Rho GTPase activating prote... NM_153800.4 79.7% 34.8% (many diffs)
42 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_011245053.2 79.7% 34.8% (many diffs)
43 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_006518957.3 79.4% 35.6% (many diffs)
44 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_017316004.1 78.1% 33.4% (many diffs)
45 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_006518956.3 77.9% 34% (many diffs)
46 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_006518954.3 76.4% 32.7% (many diffs)
47 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_017316006.1 72.7% 27.8% (many diffs)
48 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_006518961.3 69.9% 22.8% (many diffs)
49 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_006518962.3 69.9% 22.8% (many diffs)
50 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_006518959.2 68.4% 22.3% (many diffs)
51 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_006518960.3 68.4% 22.3% (many diffs)
52 mouse 239027 Arhgap22 Rho GTPase activating prote... XM_017316007.1 40.3% 62.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
882
ORF length:
813
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gctgagccca aagatcaggc aggccaggag ggcccgctcc aaaagcctag
121tgatggggga gcagagccgg agccctgggc ggatgccgtg ccctcacagg ctgggccccg
181tgctgaaggc gggctggctg aagaagcaga ggagcatcat gaagaactgg cagcagcgct
241ggtttgtgct gcgtggggat cagcttttct actacaagga caaagatgag atcaagcccc
301agggatttat ttctctacaa gggacacagg tgactgaact tcctcctggc cccgaggacc
361cagggaagca cctctttgag atcagcccag gtggtgctgg ggagcgggag aaggtgccgg
421ccaaccccga ggcgctcctg ctcatggcca gctcccagcg tgacatggag gactgggtgc
481aggccatccg ccgagtcatc tgggccccgc tgggcggagg gatctttggg cagcgcctag
541aggaaacagt ccaccacgag cggaagtatg gcccccgcct ggcgcacctg ctggtggagc
601agtgtgtgga cttcatccgg gagcgcgggc tcactgagga ggggctgttc cgcatgccgg
661gccaggccaa cctggtgagg gacctgcagg attccttcga ctgtggggag aagccactgt
721ttgacagcac aacagacgtg cacacggtgg cctccctgct gaagctgtac ctgcgggagc
781tccccgagcc cgtggtcccc ttcgccaggt acgaggactt cctcagctgc gcccagctgc
841tcaccaagga cgagggggag ggcactctgg agttggctaa ataagtgagc aaccttcctc
901aggcaaatta caacctgctc agatacatct gcaagtttct ggatgaagtt caggcatact
961caaatgtcaa caagatgagt gtccagaatc tggcaaccgt ttttggacct aacattctgc
1021ggccacaggt agaggaccca gtaaccatca tggaaggcac ttccctcgtc cagcacctga
1081tgaccgtcct catccgcaaa cacagccagc tcttcacggc accggtcccg gaagggccca
1141cctccccgcg cgggggcctg caatgcgcag tggggtgggg ctccgaggag gtcaccaggg
1201acagccaagg agagcccggc ggccccggcc tgcccgcgca caggacctct tccctggacg
1261gggcggccgt ggcggtgctc tccagaacag cccccaaggg gccggggagc cggtgcagcc
1321ctgggaagaa ggtgcagacc ctgcccagtt ggaagtcctc cttccggcag ccgaggtccc
1381tatcgggaag cccgaagggg ggcggctcat ccctggaggt gcccatcatc tcctccggcg
1441ggaactggct tatgaacggg ctgtcctccc tgcgcggaca ccgccgggcc tcgtcgggag
1501accggctcaa ggactcgggc tccgtgcaga gactctccac ctacgacaat gtgcccgcgc
1561cgggcctggt ccccggcata cccagcgtgg ccagtatggc gtggtccggg gcctcgtcca
1621gcgagtcgtc ggtggggggc tcactcagca gctgcacggc ctgccgcgcc agcgactcgt
1681ctgcccgcag ttccctgcac accgactggg ccctggagcc ctccccgctc cccagcagca
1741gcgaggaccc caagtccctg gacctggacc acagcatgga cgaggcgggc gcgggtgcca
1801gcaacagcga gccaagcgag ccggacagcc ccacccggga acacgcgcgc cgctccgagg
1861ccttacaggg gctggtcact gagcTCAGGG CCGAGCTGTG CCGCCAGCGG ACTGAGTACG
1921AGAGGAGTGT GAAAAGAATC GAAGAAGGGA GTGCTGACCT GAGAAAACGA ATGTCCCGGT
1981TAGAAGAAGA ACTGGACCAG GAAAAGAAAA AATACATCAT GCTGGAAATA AAGCTGCGGG
2041ACTCTGAACG GGCGCGGGAG GATGCGGAGA GGAGGAACCA GCTGTTGCAG AGGGAAATGG
2101AGGAGTTTTT TTCGACCCTA GGAAGCTTGA CTGTTGGGGC AAAAGGTGCC AGGGCCCCAA
2161AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
2221TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
2281GGCTTTATAT ATCTTGTGGA AAGGACGAGA GTTTAGGGGT TTCCTCCTTG GCACGCGTTA
2341AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)