Transcript: Mouse XM_006520119.3

PREDICTED: Mus musculus serine/threonine kinase 3 (Stk3), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Stk3 (56274)
Length:
2004
CDS:
344..1780

Additional Resources:

NCBI RefSeq record:
XM_006520119.3
NBCI Gene record:
Stk3 (56274)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147084 TGGATTGTTATGGAGTACTG pXPR_003 TGG 302 21% 4 0.9022 Stk3, Stk4 STK3 75975
2 BRDN0001145644 GGATGTATATCAGCATAAGG pXPR_003 AGG 660 46% 6 0.4331 Stk3 STK3 75976
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006520119.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274761 TACATCCGATGAGGGCTATTT pLKO_005 1014 CDS 100% 13.200 18.480 N Stk3 n/a
2 TRCN0000025960 CCGGGAATATTCTCCTCAATA pLKO.1 789 CDS 100% 13.200 9.240 N Stk3 n/a
3 TRCN0000274762 CCGGGAATATTCTCCTCAATA pLKO_005 789 CDS 100% 13.200 9.240 N Stk3 n/a
4 TRCN0000025880 CCTTCTTTCATGGACTACTTT pLKO.1 1532 CDS 100% 5.625 3.938 N Stk3 n/a
5 TRCN0000025947 GCAATTAAGCAAGTACCTGTT pLKO.1 503 CDS 100% 4.050 2.835 N Stk3 n/a
6 TRCN0000025951 CCTGAGGTAATTCAAGAAATA pLKO.1 911 CDS 100% 13.200 7.920 N Stk3 n/a
7 TRCN0000323443 CCTGAGGTAATTCAAGAAATA pLKO_005 911 CDS 100% 13.200 7.920 N Stk3 n/a
8 TRCN0000002177 CGGATGAAGATGAGCTGGATT pLKO.1 1290 CDS 100% 4.950 2.970 N STK3 n/a
9 TRCN0000315193 CGGATGAAGATGAGCTGGATT pLKO_005 1290 CDS 100% 4.950 2.970 N STK3 n/a
10 TRCN0000087583 AGGAGGAAGAGGAAGAGGAAA pLKO.1 1458 CDS 100% 4.950 2.475 Y Adam32 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006520119.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14850 pDONR223 0% 82.9% 84.6% None (many diffs) n/a
2 ccsbBroad304_14850 pLX_304 20.5% 82.9% 84.6% V5 (many diffs) n/a
3 TRCN0000470542 TGTCTGCAAGTCTCTCTATTTCCC pLX_317 26.5% 82.9% 84.6% V5 (many diffs) n/a
4 TRCN0000491248 TCAACTCTAATCTCTCTTATATGC pLX_317 13.9% 82.9% 84.6% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000488127 AACTCAATGCCCTACCTTGTAGCA pLX_317 20.6% 82.8% 84.4% V5 (many diffs) n/a
6 ccsbBroadEn_07012 pDONR223 100% 82.8% 84.4% None (many diffs) n/a
7 ccsbBroad304_07012 pLX_304 20.5% 82.8% 84.4% V5 (many diffs) n/a
8 TRCN0000475289 ACCGACTTGTTGACGCTCCGTCAA pLX_317 20.2% 82.8% 84.4% V5 (many diffs) n/a
Download CSV