Construct: ORF TRCN0000470542
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005609.2_s317c1
- Derived from:
- ccsbBroadEn_14850
- DNA Barcode:
- TGTCTGCAAGTCTCTCTATTTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK3 (6788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470542
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6788 | STK3 | serine/threonine kinase 3 | NM_006281.4 | 100% | 100% | |
| 2 | human | 6788 | STK3 | serine/threonine kinase 3 | NM_001256312.2 | 94.2% | 93% | (many diffs) |
| 3 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013758.1 | 91.4% | 88.9% | (many diffs) |
| 4 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517251.2 | 89.1% | 81.9% | (many diffs) |
| 5 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517248.2 | 84.8% | 83.8% | (many diffs) |
| 6 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013761.2 | 82% | 82% | 683_684ins264 |
| 7 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013756.1 | 77.8% | 75.3% | (many diffs) |
| 8 | human | 6788 | STK3 | serine/threonine kinase 3 | NM_001256313.1 | 77.3% | 77.3% | 351_352ins333 |
| 9 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013757.1 | 75.8% | 74.8% | (many diffs) |
| 10 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013759.2 | 70.1% | 59.8% | (many diffs) |
| 11 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_024447238.1 | 70.1% | 59.8% | (many diffs) |
| 12 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013760.2 | 69.4% | 61.3% | (many diffs) |
| 13 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517252.3 | 65.6% | 64.5% | (many diffs) |
| 14 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_024447239.1 | 63.5% | 57.1% | (many diffs) |
| 15 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517255.3 | 55.4% | 43.4% | (many diffs) |
| 16 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517258.2 | 45.5% | 39.5% | (many diffs) |
| 17 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013764.2 | 45.5% | 39.5% | (many diffs) |
| 18 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517257.2 | 39.2% | 38.1% | (many diffs) |
| 19 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013762.2 | 39.2% | 38.1% | (many diffs) |
| 20 | human | 6788 | STK3 | serine/threonine kinase 3 | XR_001745584.1 | 25.7% | (many diffs) | |
| 21 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | NM_019635.2 | 89% | 95.7% | (many diffs) |
| 22 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | XM_006520119.3 | 82.9% | 84.6% | (many diffs) |
| 23 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | XM_006520120.3 | 80.1% | 85.7% | (many diffs) |
| 24 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | XM_006520121.3 | 75.2% | 81.2% | (many diffs) |
| 25 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | XM_011245368.2 | 70.5% | 76.2% | (many diffs) |
| 26 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | XM_011245369.2 | 63% | 68.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1539
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcagccgccg gcgcctaaga gtaaactaaa aaagctgagt gaagacagtt 121 tgactaagca gcctgaagaa gtttttgatg tattagagaa gcttggagaa gggtcttatg 181 gaagtgtatt taaagcaata cacaaggaat ccggtcaagt tgtcgcaatt aaacaagtac 241 ctgttgaatc agatcttcag gaaataatca aagaaatttc cataatgcag caatgtgaca 301 gcccatatgt tgtaaagtac tatggcagtt attttaagaa tacagacctc tggattgtta 361 tggagtactg tggcgctggc tctgtctcag acataattag attacgaaac aagacattaa 421 tagaagatga aattgcaacc attcttaaat ctacattgaa aggactagaa tatttgcact 481 ttatgagaaa aatacacaga gatataaaag ctggaaatat tctcctcaat acagaaggac 541 atgcaaaatt ggcagatttt ggagtggctg gtcagttaac agatacaatg gcaaaacgca 601 atactgtaat aggaactcca ttttggatgg ctcctgaggt gattcaagaa ataggctata 661 actgtgtggc cgacatctgg tcccttggca ttacttctat agaaatggct gaaggaaaac 721 ctccttatgc tgatatacat ccaatgaggg ctatttttat gattcccaca aatccaccac 781 caacattcag aaagccagaa ctttggtccg atgatttcac cgattttgtt aaaaagtgtt 841 tggtgaagaa tcctgagcag agagctactg caacacaact tttacagcat ccttttatca 901 agaatgccaa acctgtatca atattaagag acctgatcac agaagctatg gagatcaaag 961 ctaaaagaca tgaggaacag caacgagaat tggaagagga agaagaaaat tcggatgaag 1021 atgagctgga ttcccacacc atggtgaaga ctagtgtgga gagtgtgggc accatgcggg 1081 ccacaagcac gatgagtgaa ggggcccaga ccatgattga acataatagc acgatgttgg 1141 aatccgactt gggGACCATG GTGATAAACA GTGAGGATGA GGAAGAAGAA GATGGAACTA 1201 TGAAAAGAAA TGCAACCTCA CCACAAGTAC AAAGACCATC TTTCATGGAC TACTTTGATA 1261 AGCAAGACTT CAAGAATAAG AGTCACGAAA ACTGTAATCA GAACATGCAT GAACCCTTCC 1321 CTATGTCCAA AAACGTTTTT CCTGATAACT GGAAAGTTCC TCAAGATGGA GACTTTGACT 1381 TTTTGAAAAA TCTAAGTTTA GAAGAACTAC AGATGCGGTT AAAAGCACTG GACCCCATGA 1441 TGGAACGGGA GATAGAAGAA CTTCGTCAGA GATACACTGC GAAAAGACAG CCCATTCTGG 1501 ATGCGATGGA TGCAAAGAAA AGAAGGCAGC AAAACTTTTG CCCAACTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGATGTCTG CAAGTCTCTC TATTTCCCAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt