Construct: ORF TRCN0000488127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019721.1_s317c1
- DNA Barcode:
- AACTCAATGCCCTACCTTGTAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK3 (6788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6788 | STK3 | serine/threonine kinase 3 | NM_006281.4 | 99.9% | 99.7% | 1473_1474insG |
| 2 | human | 6788 | STK3 | serine/threonine kinase 3 | NM_001256312.2 | 94.1% | 92.8% | (many diffs) |
| 3 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013758.1 | 91.4% | 88.7% | (many diffs) |
| 4 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517251.2 | 89.1% | 81.9% | (many diffs) |
| 5 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517248.2 | 84.8% | 83.7% | (many diffs) |
| 6 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013761.2 | 82% | 81.9% | 683_684ins264;1209_1210insG |
| 7 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013756.1 | 77.8% | 75.2% | (many diffs) |
| 8 | human | 6788 | STK3 | serine/threonine kinase 3 | NM_001256313.1 | 77.3% | 77.2% | 351_352ins333;1140_1141insG |
| 9 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013757.1 | 75.8% | 74.6% | (many diffs) |
| 10 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013759.2 | 70.2% | 59.7% | (many diffs) |
| 11 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_024447238.1 | 70.2% | 59.7% | (many diffs) |
| 12 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013760.2 | 69.5% | 61.2% | (many diffs) |
| 13 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517252.3 | 65.5% | 64.4% | (many diffs) |
| 14 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_024447239.1 | 63.4% | 57% | (many diffs) |
| 15 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517255.3 | 55.5% | 43.2% | (many diffs) |
| 16 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517258.2 | 45.5% | 39.2% | (many diffs) |
| 17 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013764.2 | 45.5% | 39.2% | (many diffs) |
| 18 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_011517257.2 | 39.2% | 38.1% | (many diffs) |
| 19 | human | 6788 | STK3 | serine/threonine kinase 3 | XM_017013762.2 | 39.2% | 38.1% | (many diffs) |
| 20 | human | 6788 | STK3 | serine/threonine kinase 3 | XR_001745584.1 | 25.7% | (many diffs) | |
| 21 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | NM_019635.2 | 89% | 95.5% | (many diffs) |
| 22 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | XM_006520119.3 | 82.8% | 84.4% | (many diffs) |
| 23 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | XM_006520120.3 | 80% | 85.5% | (many diffs) |
| 24 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | XM_006520121.3 | 75.1% | 81.1% | (many diffs) |
| 25 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | XM_011245368.2 | 70.4% | 76.1% | (many diffs) |
| 26 | mouse | 56274 | Stk3 | serine/threonine kinase 3 | XM_011245369.2 | 63% | 68% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1548
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagcag ccgccggcgc ctaagagtaa actaaaaaag ctgagtgaag 121 acagtttgac taagcagcct gaagaagttt ttgatgtatt agagaagctt ggagaagggt 181 cttatggaag tgtatttaaa gcaatacaca aggaatccgg tcaagttgtc gcaattaaac 241 aagtacctgt tgaatcagat cttcaggaaa taatcaaaga aatttccata atgcagcaat 301 gtgacagccc atatgttgta aagtactatg gcagttattt taagaataca gacctctgga 361 ttgttatgga gtactgtggc gctggctctg tctcagacat aattagatta cgaaacaaga 421 cattaataga agatgaaatt gcaaccattc ttaaatctac attgaaagga ctagaatatt 481 tgcactttat gagaaaaata cacagagata taaaagctgg aaatattctc ctcaatacag 541 aaggacatgc aaaattggca gattttggag tggctggtca gttaacagat acaatggcaa 601 aacgcaatac tgtaatagga actccatttt ggatggctcc tgaggtgatt caagaaatag 661 gctataactg tgtggccgac atctggtccc ttggcattac ttctatagaa atggctgaag 721 gaaaacctcc ttatgctgat atacatccaa tgagggctat ttttatgatt cccacaaatc 781 caccaccaac attcagaaag ccagaacttt ggtccgatga tttcaccgat tttgttaaaa 841 agtgtttggt gaagaatcct gagcagagag ctactgcaac acaactttta cagcatcctt 901 ttatcaagaa tgccaaacct gtatcaatat taagagacct gatcacagaa gctatggaga 961 tcaaagctaa aagacatgag gaacagcaac gagaattgga agaggaagaa gaaaattcgg 1021 atgaagatga gctggattcc cacaccatgg tgaagactag tgtggagagt gtgggcacca 1081 tgcgggccac aagcacgatg agtgaagggg cccagaccat gattgaacat aatagcacga 1141 tgttggaatc cgacttgggg accatggtga taaacagtga ggatgaggaa gaagaagatg 1201 gaactatgaa aagaaatgca acctcaccac aagtacaaag accatcTTTC ATGGACTACT 1261 TTGATAAGCA AGACTTCAAG AATAAGAGTC ACGAAAACTG TAATCAGAAC ATGCATGAAC 1321 CCTTCCCTAT GTCCAAAAAC GTTTTTCCTG ATAACTGGAA AGTTCCTCAA GATGGAGACT 1381 TTGACTTTTT GAAAAATCTA AGTTTAGAAG AACTACAGAT GCGGTTAAAA GCACTGGACC 1441 CCATGATGGA ACGGGAGATA GAAGAACTTC GTCAGAGATA CACTGCGAAA AGACAGCCCA 1501 TTCTGGATGC GATGGATGCA AAGAAAAGAA GGCAGCAAAA CTTTGACCCA GCTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA AACTCAATGC CCTACCTTGT AGCAACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt