Construct: ORF TRCN0000475289

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005609.3_s317c1
Derived from:
ccsbBroadEn_07012
DNA Barcode:
ACCGACTTGTTGACGCTCCGTCAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STK3 (6788)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475289
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6788 STK3 serine/threonine kinase 3 NM_006281.4 99.9% 99.7% 10C>A
2 human 6788 STK3 serine/threonine kinase 3 NM_001256312.2 93.5% 93% (many diffs)
3 human 6788 STK3 serine/threonine kinase 3 XM_017013758.1 91.4% 88.7% (many diffs)
4 human 6788 STK3 serine/threonine kinase 3 XM_011517251.2 89% 81.8% (many diffs)
5 human 6788 STK3 serine/threonine kinase 3 XM_011517248.2 84.3% 83.8% (many diffs)
6 human 6788 STK3 serine/threonine kinase 3 XM_017013761.2 82% 81.8% 10C>A;683_684ins264
7 human 6788 STK3 serine/threonine kinase 3 NM_001256313.1 77.3% 77.1% 10C>A;351_352ins333
8 human 6788 STK3 serine/threonine kinase 3 XM_017013756.1 77.2% 75.3% (many diffs)
9 human 6788 STK3 serine/threonine kinase 3 XM_017013757.1 75.3% 74.8% (many diffs)
10 human 6788 STK3 serine/threonine kinase 3 XM_017013759.2 70.1% 59.8% (many diffs)
11 human 6788 STK3 serine/threonine kinase 3 XM_024447238.1 70.1% 59.8% (many diffs)
12 human 6788 STK3 serine/threonine kinase 3 XM_017013760.2 69.4% 61.3% (many diffs)
13 human 6788 STK3 serine/threonine kinase 3 XM_011517252.3 65.1% 64.5% (many diffs)
14 human 6788 STK3 serine/threonine kinase 3 XM_024447239.1 63.5% 57.1% (many diffs)
15 human 6788 STK3 serine/threonine kinase 3 XM_011517255.3 55.4% 43.4% (many diffs)
16 human 6788 STK3 serine/threonine kinase 3 XM_011517258.2 45.5% 39.5% (many diffs)
17 human 6788 STK3 serine/threonine kinase 3 XM_017013764.2 45.5% 39.5% (many diffs)
18 human 6788 STK3 serine/threonine kinase 3 XM_011517257.2 38.8% 38.1% (many diffs)
19 human 6788 STK3 serine/threonine kinase 3 XM_017013762.2 38.8% 38.1% (many diffs)
20 human 6788 STK3 serine/threonine kinase 3 XR_001745584.1 25.6% (many diffs)
21 mouse 56274 Stk3 serine/threonine kinase 3 NM_019635.2 89% 95.5% (many diffs)
22 mouse 56274 Stk3 serine/threonine kinase 3 XM_006520119.3 82.8% 84.4% (many diffs)
23 mouse 56274 Stk3 serine/threonine kinase 3 XM_006520120.3 80% 85.5% (many diffs)
24 mouse 56274 Stk3 serine/threonine kinase 3 XM_006520121.3 75.2% 81.2% (many diffs)
25 mouse 56274 Stk3 serine/threonine kinase 3 XM_011245368.2 70.5% 76.2% (many diffs)
26 mouse 56274 Stk3 serine/threonine kinase 3 XM_011245369.2 63% 68.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1539
ORF length:
1473
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gcagacgccg gcgcctaaga gtaaactaaa aaagctgagt gaagacagtt
121tgactaagca gcctgaagaa gtttttgatg tattagagaa gcttggagaa gggtcttatg
181gaagtgtatt taaagcaata cacaaggaat ccggtcaagt tgtcgcaatt aaacaagtac
241ctgttgaatc agatcttcag gaaataatca aagaaatttc cataatgcag caatgtgaca
301gcccatatgt tgtaaagtac tatggcagtt attttaagaa tacagacctc tggattgtta
361tggagtactg tggcgctggc tctgtctcag acataattag attacgaaac aagacattaa
421tagaagatga aattgcaacc attcttaaat ctacattgaa aggactagaa tatttgcact
481ttatgagaaa aatacacaga gatataaaag ctggaaatat tctcctcaat acagaaggac
541atgcaaaatt ggcagatttt ggagtggctg gtcagttaac agatacaatg gcaaaacgca
601atactgtaat aggaactcca ttttggatgg ctcctgaggt gattcaagaa ataggctata
661actgtgtggc cgacatctgg tcccttggca ttacttctat agaaatggct gaaggaaaac
721ctccttatgc tgatatacat ccaatgaggg ctatttttat gattcccaca aatccaccac
781caacattcag aaagccagaa ctttggtccg atgatttcac cgattttgtt aaaaagtgtt
841tggtgaagaa tcctgagcag agagctactg caacacaact tttacagcat ccttttatca
901agaatgccaa acctgtatca atattaagag acctgatcac agaagctatg gagatcaaag
961ctaaaagaca tgaggaacag caacgagaat tggaagagga agaagaaaat tcggatgaag
1021atgagctgga ttcccacacc atggtgaaga ctagtgtgga gagtgtgggc accatgcggg
1081ccacaagcac gatgagtgaa ggggcccaga ccatgattga acataatagc acgatgttgg
1141aatccgactt ggggaccatg gtgataaaca gtgaggatga ggaagaagaa gatggaacta
1201tgaaaagaaa tgcaacctca ccacaagtac aaagaccatc tttcatGGAC TACTTTGATA
1261AGCAAGACTT CAAGAATAAG AGTCACGAAA ACTGTAATCA GAACATGCAT GAACCCTTCC
1321CTATGTCCAA AAACGTTTTT CCTGATAACT GGAAAGTTCC TCAAGATGGA GACTTTGACT
1381TTTTGAAAAA TCTAAGTTTA GAAGAACTAC AGATGCGGTT AAAAGCACTG GACCCCATGA
1441TGGAACGGGA GATAGAAGAA CTTCGTCAGA GATACACTGC GAAAAGACAG CCCATTCTGG
1501ATGCGATGGA TGCAAAGAAA AGAAGGCAGC AAAACTTTTG CCCAACTTTC TTGTACAAAG
1561TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1621GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1681ACGAACCGAC TTGTTGACGC TCCGTCAAAC GCGTTAAGTC gacaatcaac ctctggatta
1741caaaatttgt gaaagatt

Download FASTA (ORF) (Full)