Transcript: Mouse XM_006521665.2

PREDICTED: Mus musculus zinc finger CCCH type containing 7 A (Zc3h7a), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Zc3h7a (106205)
Length:
3771
CDS:
163..3075

Additional Resources:

NCBI RefSeq record:
XM_006521665.2
NBCI Gene record:
Zc3h7a (106205)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006521665.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000350733 TATGAATGGACCAGGTAATTT pLKO_005 1332 CDS 100% 15.000 21.000 N Zc3h7a n/a
2 TRCN0000322505 TTTGATCTATGCAACCATATT pLKO_005 2473 CDS 100% 13.200 10.560 N Zc3h7a n/a
3 TRCN0000216154 CACCGCTTAACAACAGTAATT pLKO.1 1298 CDS 100% 13.200 9.240 N Zc3h7a n/a
4 TRCN0000322504 ATCTGTATGTAGGTTGCTAAA pLKO_005 3477 3UTR 100% 10.800 7.560 N Zc3h7a n/a
5 TRCN0000217650 GCAGATGATGAATGCCGATAT pLKO.1 1690 CDS 100% 10.800 7.560 N Zc3h7a n/a
6 TRCN0000322503 TGTCGACATGAAGTTCGTTAT pLKO_005 2062 CDS 100% 10.800 7.560 N Zc3h7a n/a
7 TRCN0000191800 GCAACCATATTGCTAATAGAA pLKO.1 2483 CDS 100% 5.625 3.938 N Zc3h7a n/a
8 TRCN0000201879 GCTGCAGATTTCTGCTTGATT pLKO.1 3152 3UTR 100% 5.625 3.938 N Zc3h7a n/a
9 TRCN0000191188 CAGAAACAAATGATGCTGTTA pLKO.1 1040 CDS 100% 4.950 3.465 N Zc3h7a n/a
10 TRCN0000322502 AGCTTTGAGTATAGCTAATTA pLKO_005 366 CDS 100% 15.000 9.000 N Zc3h7a n/a
11 TRCN0000191887 GCTCAGAAATTGGGATTTAAA pLKO.1 661 CDS 100% 15.000 9.000 N Zc3h7a n/a
12 TRCN0000190986 CGGAGTACATGATGGATGAAA pLKO.1 3401 3UTR 100% 5.625 3.375 N Zc3h7a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006521665.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11894 pDONR223 100% 15.1% 14.7% None (many diffs) n/a
2 ccsbBroad304_11894 pLX_304 0% 15.1% 14.7% V5 (many diffs) n/a
3 TRCN0000467084 ACCGATTTAAGGTTGATTACTGGG pLX_317 66.4% 15.1% 14.7% V5 (many diffs) n/a
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