Transcript: Mouse XM_006525479.2

PREDICTED: Mus musculus tumor necrosis factor, alpha-induced protein 8 (Tnfaip8), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tnfaip8 (106869)
Length:
1963
CDS:
368..946

Additional Resources:

NCBI RefSeq record:
XM_006525479.2
NBCI Gene record:
Tnfaip8 (106869)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006525479.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000348693 AGTCTCACGGACGGGTTAATA pLKO_005 798 CDS 100% 15.000 21.000 N Tnfaip8 n/a
2 TRCN0000100261 CGGACGGGTTAATAATGTCTT pLKO.1 805 CDS 100% 4.950 6.930 N Tnfaip8 n/a
3 TRCN0000100262 GCTGCCTTGTACAATCCCTTT pLKO.1 854 CDS 100% 4.050 3.240 N Tnfaip8 n/a
4 TRCN0000351747 GCTGCCTTGTACAATCCCTTT pLKO_005 854 CDS 100% 4.050 3.240 N Tnfaip8 n/a
5 TRCN0000375643 TGGCTACAGATGTCTTCAATT pLKO_005 381 CDS 100% 13.200 9.240 N Tnfaip8 n/a
6 TRCN0000375642 AGCTCCTACACGAGATCATTC pLKO_005 759 CDS 100% 10.800 7.560 N Tnfaip8 n/a
7 TRCN0000100264 CCACAGGAACAATCAGTTCAA pLKO.1 604 CDS 100% 4.950 3.465 N Tnfaip8 n/a
8 TRCN0000100263 CCTCCACAGGAACAATCAGTT pLKO.1 601 CDS 100% 4.950 3.465 N Tnfaip8 n/a
9 TRCN0000367650 CCTCCACAGGAACAATCAGTT pLKO_005 601 CDS 100% 4.950 3.465 N Tnfaip8 n/a
10 TRCN0000100260 CCTGTTATTGTTGTTGGACAT pLKO.1 1072 3UTR 100% 4.050 2.835 N Tnfaip8 n/a
11 TRCN0000351748 CCTGTTATTGTTGTTGGACAT pLKO_005 1072 3UTR 100% 4.050 2.835 N Tnfaip8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006525479.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000472974 GCGAACAGCTTTCGCACATATTAC pLX_317 88.5% 83.3% 89.8% V5 (many diffs) n/a
2 ccsbBroadEn_14094 pDONR223 100% 83.1% 87.3% None (many diffs) n/a
3 ccsbBroad304_14094 pLX_304 0% 83.1% 87.3% V5 (not translated due to frame shift) (many diffs) n/a
4 ccsbBroadEn_07947 pDONR223 100% 83.1% 89.3% None (many diffs) n/a
5 ccsbBroad304_07947 pLX_304 0% 83.1% 89.3% V5 (many diffs) n/a
6 TRCN0000468140 CCTAGATTGTGCATGTAGGAACAA pLX_317 49% 83.1% 89.3% V5 (many diffs) n/a
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