Transcript: Mouse XM_006525489.1

PREDICTED: Mus musculus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (Arap3), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Arap3 (106952)
Length:
5273
CDS:
108..4724

Additional Resources:

NCBI RefSeq record:
XM_006525489.1
NBCI Gene record:
Arap3 (106952)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006525489.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000106136 CCACGGGAACACTTTGTAGAA pLKO.1 2997 CDS 100% 4.950 6.930 N Arap3 n/a
2 TRCN0000288346 CCACGGGAACACTTTGTAGAA pLKO_005 2997 CDS 100% 4.950 6.930 N Arap3 n/a
3 TRCN0000295678 GCAGAAGTGTGCGTCTCTAAA pLKO_005 3218 CDS 100% 13.200 10.560 N Arap3 n/a
4 TRCN0000295677 CTCCGGCTGGAAGGTGTATAT pLKO_005 2898 CDS 100% 13.200 9.240 N Arap3 n/a
5 TRCN0000106135 CTGTACTATGAAGTCACTAAA pLKO.1 4931 3UTR 100% 13.200 9.240 N Arap3 n/a
6 TRCN0000288345 CTGTACTATGAAGTCACTAAA pLKO_005 4931 3UTR 100% 13.200 9.240 N Arap3 n/a
7 TRCN0000369919 GGGAATCCGCAAGAAGTTAAA pLKO_005 3935 CDS 100% 13.200 9.240 N ARAP3 n/a
8 TRCN0000295613 TGTATGAAGAGCCAGTATATG pLKO_005 4300 CDS 100% 13.200 9.240 N Arap3 n/a
9 TRCN0000106138 CCTGGATTCTCATCTGGATAA pLKO.1 317 CDS 100% 10.800 7.560 N Arap3 n/a
10 TRCN0000106139 GCAGAGCTTTCCCAGAAGAAT pLKO.1 3105 CDS 100% 5.625 3.938 N Arap3 n/a
11 TRCN0000106137 GCTGTTCATTGTCCTGGGAAA pLKO.1 1763 CDS 100% 4.050 2.835 N Arap3 n/a
12 TRCN0000047344 GTCTGGAGTAATGAGATAGTA pLKO.1 1740 CDS 100% 5.625 7.875 N ARAP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006525489.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12469 pDONR223 100% 4.4% 1.2% None (many diffs) n/a
2 ccsbBroad304_12469 pLX_304 0% 4.4% 1.2% V5 (many diffs) n/a
3 TRCN0000467121 TACAGAGCGCGACATTTACCCTTA pLX_317 100% 4.4% 1.2% V5 (many diffs) n/a
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