Transcript: Mouse XM_006527803.1

PREDICTED: Mus musculus four and a half LIM domains 1 (Fhl1), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fhl1 (14199)
Length:
2640
CDS:
250..1221

Additional Resources:

NCBI RefSeq record:
XM_006527803.1
NBCI Gene record:
Fhl1 (14199)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006527803.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000306158 TGCCAAGGAGGTGCATTATAA pLKO_005 399 CDS 100% 15.000 21.000 N Fhl1 n/a
2 TRCN0000113521 CATGAGACCAAGTTCGCCAAA pLKO.1 709 CDS 100% 4.050 5.670 N Fhl1 n/a
3 TRCN0000306157 CGCTGTGGAGGACCAGTATTA pLKO_005 852 CDS 100% 13.200 9.240 N Fhl1 n/a
4 TRCN0000113523 AGCAACTGCAAGCAAGTCATT pLKO.1 637 CDS 100% 4.950 3.465 N Fhl1 n/a
5 TRCN0000326111 AGCAACTGCAAGCAAGTCATT pLKO_005 637 CDS 100% 4.950 3.465 N Fhl1 n/a
6 TRCN0000113522 CTGCCTGAAGTGCTTTGACAA pLKO.1 330 CDS 100% 4.950 3.465 N Fhl1 n/a
7 TRCN0000326184 CTGCCTGAAGTGCTTTGACAA pLKO_005 330 CDS 100% 4.950 3.465 N Fhl1 n/a
8 TRCN0000117826 TGGTGGCCTATGAAGGACAAT pLKO.1 1159 CDS 100% 4.950 3.465 N FHL1 n/a
9 TRCN0000113524 CTTTGACAAGTTCTGCGCCAA pLKO.1 342 CDS 100% 2.160 1.512 N Fhl1 n/a
10 TRCN0000117823 CGTGGATTGCTACAAGAACTT pLKO.1 876 CDS 100% 4.950 2.970 N FHL1 n/a
11 TRCN0000287068 CGTGGATTGCTACAAGAACTT pLKO_005 876 CDS 100% 4.950 2.970 N FHL1 n/a
12 TRCN0000113520 GCAGTTAAGAACTTGCCAGAA pLKO.1 1742 3UTR 100% 4.050 2.430 N Fhl1 n/a
13 TRCN0000326183 GCAGTTAAGAACTTGCCAGAA pLKO_005 1742 3UTR 100% 4.050 2.430 N Fhl1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006527803.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00567 pDONR223 100% 69.6% 70.5% None (many diffs) n/a
2 ccsbBroad304_00567 pLX_304 0% 69.6% 70.5% V5 (many diffs) n/a
3 TRCN0000470524 TCCCGGTGTGGCCGGAGATGCCTC pLX_317 38.4% 69.6% 70.5% V5 (many diffs) n/a
Download CSV