Construct: ORF TRCN0000470524

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009299.1_s317c1
Derived from:
ccsbBroadEn_00567
DNA Barcode:
TCCCGGTGTGGCCGGAGATGCCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FHL1 (2273)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470524
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2273 FHL1 four and a half LIM domains 1 NM_001159700.2 100% 100%
2 human 2273 FHL1 four and a half LIM domains 1 NM_001159704.1 100% 100%
3 human 2273 FHL1 four and a half LIM domains 1 NM_001167819.1 100% 100%
4 human 2273 FHL1 four and a half LIM domains 1 NM_001369329.1 100% 100%
5 human 2273 FHL1 four and a half LIM domains 1 NM_001369330.1 100% 100%
6 human 2273 FHL1 four and a half LIM domains 1 NM_001369331.1 100% 100%
7 human 2273 FHL1 four and a half LIM domains 1 NM_001449.5 100% 100%
8 human 2273 FHL1 four and a half LIM domains 1 NM_001159699.2 94.5% 94.5% 1_48del
9 human 2273 FHL1 four and a half LIM domains 1 NM_001159701.2 90.6% 90.6% 1_87del
10 human 2273 FHL1 four and a half LIM domains 1 NM_001159702.3 73.9% 74.6% 688_887del;969_970ins71
11 human 2273 FHL1 four and a half LIM domains 1 NM_001369326.1 73.9% 74.6% 688_887del;969_970ins71
12 human 2273 FHL1 four and a half LIM domains 1 NM_001369327.1 73.9% 74.6% 688_887del;969_970ins71
13 human 2273 FHL1 four and a half LIM domains 1 NM_001369328.1 73.9% 74.6% 688_887del;969_970ins71
14 human 2273 FHL1 four and a half LIM domains 1 XM_006724746.3 73.9% 74.6% 688_887del;969_970ins71
15 human 2273 FHL1 four and a half LIM domains 1 XM_024452354.1 73.9% 74.6% 688_887del;969_970ins71
16 human 2273 FHL1 four and a half LIM domains 1 XM_006724743.2 70.6% 71% 1_48del;736_935del;1017_1018ins71
17 human 2273 FHL1 four and a half LIM domains 1 NM_001159703.2 69.2% 57.8% 500_501ins187;582_583ins71
18 human 2273 FHL1 four and a half LIM domains 1 NM_001330659.2 65.5% 54.8% 1_48del;548_549ins187;630_631ins71
19 human 2273 FHL1 four and a half LIM domains 1 NR_027621.1 32.2% 1_411del;1252_2606del
20 human 100128164 LOC100128164 four and a half LIM domains... NR_027622.1 12.8% (many diffs)
21 mouse 14199 Fhl1 four and a half LIM domains 1 NM_001287800.1 93.6% 95% (many diffs)
22 mouse 14199 Fhl1 four and a half LIM domains 1 NM_010211.3 93.6% 95% (many diffs)
23 mouse 14199 Fhl1 four and a half LIM domains 1 XM_017318381.1 93.6% 95% (many diffs)
24 mouse 14199 Fhl1 four and a half LIM domains 1 NM_001077362.2 88.6% 89.8% (many diffs)
25 mouse 14199 Fhl1 four and a half LIM domains 1 XM_006527807.2 77% 77.5% (many diffs)
26 mouse 14199 Fhl1 four and a half LIM domains 1 NM_001077361.1 69.6% 70.5% (many diffs)
27 mouse 14199 Fhl1 four and a half LIM domains 1 XM_006527800.3 69.6% 70.5% (many diffs)
28 mouse 14199 Fhl1 four and a half LIM domains 1 XM_006527802.1 69.6% 70.5% (many diffs)
29 mouse 14199 Fhl1 four and a half LIM domains 1 XM_006527803.1 69.6% 70.5% (many diffs)
30 mouse 14199 Fhl1 four and a half LIM domains 1 XM_006527804.1 69.6% 70.5% (many diffs)
31 mouse 14199 Fhl1 four and a half LIM domains 1 XM_006527805.2 69.6% 70.5% (many diffs)
32 mouse 14199 Fhl1 four and a half LIM domains 1 XM_006527801.1 66.5% 67.2% (many diffs)
33 mouse 14199 Fhl1 four and a half LIM domains 1 XM_006527808.2 64% 53.7% (many diffs)
34 mouse 14199 Fhl1 four and a half LIM domains 1 XM_017318382.1 64% 53.7% (many diffs)
35 mouse 14199 Fhl1 four and a half LIM domains 1 XM_006527809.2 60.5% 50.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
906
ORF length:
840
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggagaagttt gactgccact actgcaggga tcccttgcag gggaagaagt
121atgtgcaaaa ggatggccac cactgctgcc tgaaatgctt tgacaagttc tgtgccaaca
181cctgtgtgga atgccgcaag cccatcggtg cggactccaa ggaggtgcac tataagaacc
241gcttctggca tgacacctgc ttccgctgtg ccaagtgcct tcaccccttg gccaatgaga
301cctttgtggc caaggacaac aagatcctgt gcaacaagtg caccactcgg gaggactccc
361ccaagtgcaa ggggtgcttc aaggccattg tggcaggaga tcaaaacgtg gagtacaagg
421ggaccgtctg gcacaaagac tgcttcacct gtagtaactg caagcaagtc atcgggactg
481gaagcttctt ccctaaaggg gaggacttct actgcgtgac ttgccatgag accaagtttg
541ccaagcattg cgtgaagtgc aacaaggcca tcacatctgg aggaaTCACT TACCAGGATC
601AGCCCTGGCA TGCCGATTGC TTTGTGTGTG TTACCTGCTC TAAGAAGCTG GCTGGGCAGC
661GTTTCACCGC TGTGGAGGAC CAGTATTACT GCGTGGATTG CTACAAGAAC TTTGTGGCCA
721AGAAGTGTGC TGGATGCAAG AACCCCATCA CTGGGTTTGG TAAAGGCTCC AGTGTGGTGG
781CCTATGAAGG ACAATCCTGG CACGACTACT GCTTCCACTG CAAAAAATGC TCCGTGAATC
841TGGCCAACAA GCGCTTTGTT TTCCACCAGG AGCAAGTGTA TTGTCCCGAC TGTGCCAAAA
901AGCTGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
961TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1021CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCCCGGTGT GGCCGGAGAT GCCTCACGCG
1081TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)