Transcript: Mouse XM_006537943.3

PREDICTED: Mus musculus fukutin (Fktn), transcript variant X10, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fktn (246179)
Length:
6370
CDS:
862..1794

Additional Resources:

NCBI RefSeq record:
XM_006537943.3
NBCI Gene record:
Fktn (246179)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006537943.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000193660 CAACTATGGTAAGACCTGGAA pLKO.1 1849 3UTR 100% 2.640 3.696 N Fktn n/a
2 TRCN0000350236 CAACTATGGTAAGACCTGGAA pLKO_005 1849 3UTR 100% 2.640 3.696 N Fktn n/a
3 TRCN0000432583 AGTTACAGTTTGGTCGTTATC pLKO_005 1589 CDS 100% 10.800 8.640 N FKTN n/a
4 TRCN0000194045 GCAGTGGTACTTGCTGATAAA pLKO.1 2670 3UTR 100% 13.200 9.240 N Fktn n/a
5 TRCN0000314335 GCAGTGGTACTTGCTGATAAA pLKO_005 2670 3UTR 100% 13.200 9.240 N Fktn n/a
6 TRCN0000175490 CCTTGGAATGTGTAGTCACTA pLKO.1 3311 3UTR 100% 4.950 3.465 N Fktn n/a
7 TRCN0000314265 CCTTGGAATGTGTAGTCACTA pLKO_005 3311 3UTR 100% 4.950 3.465 N Fktn n/a
8 TRCN0000106535 GCCTGGTCTACAAAGTGAGTT pLKO.1 3463 3UTR 100% 4.950 2.475 Y Gad2 n/a
9 TRCN0000166364 CACACACACACACACACACAA pLKO.1 5186 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006537943.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00547 pDONR223 100% 46.5% 46.3% None (many diffs) n/a
2 ccsbBroad304_00547 pLX_304 0% 46.5% 46.3% V5 (many diffs) n/a
3 TRCN0000477168 TATCTTTCCCAATCGAGACGCCGT pLX_317 23.3% 46.5% 46.3% V5 (many diffs) n/a
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