Construct: ORF TRCN0000471328

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000917.1_s317c1
Derived from:
ccsbBroadEn_13864
DNA Barcode:
TCAAACTAGATTACAATTCGATTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
GRIK2 (2898)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471328
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2898 GRIK2 glutamate ionotropic recept... XM_017010782.2 96.8% 96.5% 1740delC;1749_1803delinsG
2 human 2898 GRIK2 glutamate ionotropic recept... XM_017010781.2 82.4% 82% 1740delC;1750_2121del
3 human 2898 GRIK2 glutamate ionotropic recept... NM_175768.3 67% 66.7% 1740delC;1750_2607del
4 human 2898 GRIK2 glutamate ionotropic recept... XM_024446411.1 67% 66.7% 1740delC;1750_2607del
5 human 2898 GRIK2 glutamate ionotropic recept... NM_001166247.1 65.3% 65% 1740delC;1750_2676del
6 human 2898 GRIK2 glutamate ionotropic recept... XM_011535777.3 65.3% 65% 1740delC;1750_2676del
7 human 2898 GRIK2 glutamate ionotropic recept... XM_024446410.1 65.3% 65% 1740delC;1750_2676del
8 human 2898 GRIK2 glutamate ionotropic recept... NM_021956.4 64.1% 63.8% 1740delC;1750_2724del
9 human 2898 GRIK2 glutamate ionotropic recept... XM_005266945.2 64.1% 63.8% 1740delC;1750_2724del
10 human 2898 GRIK2 glutamate ionotropic recept... XM_005266946.4 58.7% 58.4% 0_1ins147;1593delC;1603_2577del
11 human 2898 GRIK2 glutamate ionotropic recept... XR_002956278.1 35.4% 1_423del;2163delC;2173_4935del
12 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243127.2 84.5% 90.6% (many diffs)
13 mouse 14806 Grik2 glutamate receptor, ionotro... NM_010349.2 61.2% 65.4% (many diffs)
14 mouse 14806 Grik2 glutamate receptor, ionotro... XM_006512545.3 59.6% 63.9% (many diffs)
15 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243124.2 58.9% 63.1% (many diffs)
16 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243125.2 58.9% 63.1% (many diffs)
17 mouse 14806 Grik2 glutamate receptor, ionotro... XM_017313804.1 58.6% 62.8% (many diffs)
18 mouse 14806 Grik2 glutamate receptor, ionotro... NM_001111268.1 58.6% 62.6% (many diffs)
19 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243123.2 57.3% 61.5% (many diffs)
20 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243120.2 56.4% 60.5% (many diffs)
21 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243121.2 56.4% 60.5% (many diffs)
22 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243122.2 56.4% 60.5% (many diffs)
23 mouse 14806 Grik2 glutamate receptor, ionotro... XR_001779465.1 53.2% (many diffs)
24 mouse 14806 Grik2 glutamate receptor, ionotro... XR_001779464.1 47.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1806
ORF length:
1740
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gattattttc ccgattctaa gtaatccagt cttcaggcgc accgttaaac
121tcctgctctg tttactgtgg attggatatt ctcaaggaac cacacatgta ttaagatttg
181gtggtatttt tgaatatgtg gaatctggcc caatgggagc tgaggaactt gcattcagat
241ttgctgtgaa cacaattaac agaaacagaa cattgctacc caatactacc cttacctatg
301atacccagaa gataaacctt tatgatagtt ttgaagcatc caagaaagcc tgtgatcagc
361tgtctcttgg ggtggctgcc atcttcgggc cttcacacag ctcatcagca aacgcagtgc
421agtccatctg caatgctctg ggagttcccc acatacagac ccgctggaag caccaggtgt
481cagacaacaa agattccttc tatgtcagtc tctacccaga cttctcttca ctcagccgtg
541ccattttaga cctggtgcag tttttcaagt ggaaaaccgt cacggttgtg tatgatgaca
601gcactggtct cattcgtttg caagagctca tcaaagctcc atcaaggtat aatcttcgac
661tcaaaattcg tcagttacct gctgatacaa aggatgcaaa acccttacta aaagaaatga
721aaagaggcaa ggagtttcat gtaatctttg attgtagcca tgaaatggca gcaggcattt
781taaaacaggc attagctatg ggaatgatga cagaatacta tcattatatc tttaccactc
841tggacctctt tgctcttgat gttgagccct accgatacag tggtgttaac atgacagggt
901tcagaatatt aaatacagaa aatacccaag tctcctccat cattgaaaag tggtcgatgg
961aacgattgca ggcacctccg aaacccgatt caggtttgct ggatggattt atgacgactg
1021atgctgctct aatgtatgat gctgtgcatg tggtgtctgt ggccgttcaa cagtttcccc
1081agatgacagt cagttccttg cagtgtaatc gacataaacc ctggcgcttc gggacccgct
1141ttatgagtct aattaaagag gcacattggg aaggcctcac aggcagaata actttcaaca
1201aaaccaatgg cttgagaaca gattttgatt tggatgtgat cagtctgaag gaagaaggtc
1261tagaaaagat tggaacgtgg gatccagcca gtggcctgaa tatgacagaa agtcaaaagg
1321gaaagccagc gaacatcaca gattccttat ccaatcgttc tttgattgtt accaccattt
1381tggaagagcc ttatgtcctt tttaagaagt ctgacaaacc tctctatggt aatgatcgat
1441ttgaaggcta ttgcattgat ctcctcagag agttatctac aatccttggC TTTACATATG
1501AAATTAGACT TGTGGAAGAT GGGAAATATG GAGCCCAGGA TGATGCCAAT GGACAATGGA
1561ATGGAATGGT TCGTGAACTA ATTGATCATA AAGCTGACCT TGCAGTTGCT CCACTGGCTA
1621TTACCTATGT TCGAGAGAAG GTCATCGACT TTTCCAAGCC CTTTATGACA CTTGGAATAA
1681GTATTTTGTA CCGCAAGCCC AATGGTACAA ACCCAGGCGT CTTCTCCTTC CTGAATCCTC
1741TCTCCCCTGA TATCTGGATG TATATTCTGC TGGCTTACTT GGGTGTCAGT TGTGTGCTCT
1801TTGTATAGCC AGGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC
1861TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT
1921TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CAAACTAGAT TACAATTCGA
1981TTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)