Transcript: Mouse XM_011245068.2

PREDICTED: Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Nek4 (23955)
Length:
4800
CDS:
1171..3528

Additional Resources:

NCBI RefSeq record:
XM_011245068.2
NBCI Gene record:
Nek4 (23955)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011245068.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000025686 CCACAATCAGTAGGATAAATA pLKO.1 2231 CDS 100% 15.000 21.000 N Nek4 n/a
2 TRCN0000054772 CTGGGTGTGTAAACAGTTCAA pLKO.1 2762 CDS 100% 4.950 6.930 N Nek4 n/a
3 TRCN0000025688 GCTCTATGTCTGAACCTTCTT pLKO.1 2576 CDS 100% 4.950 6.930 N Nek4 n/a
4 TRCN0000025684 CCCTACAACTATAAGTCGGAT pLKO.1 1693 CDS 100% 2.640 3.696 N Nek4 n/a
5 TRCN0000025685 GCCATTGTATCCCTCAAATAA pLKO.1 2475 CDS 100% 15.000 12.000 N Nek4 n/a
6 TRCN0000054770 CAACTCTTAGAGCAGGTGTTT pLKO.1 3379 CDS 100% 4.950 3.960 N Nek4 n/a
7 TRCN0000025687 GCCTGTCTGAGGATGAGTTAA pLKO.1 3029 CDS 100% 13.200 9.240 N Nek4 n/a
8 TRCN0000054771 CCTGAGCTGTTCTCCAACAAA pLKO.1 1672 CDS 100% 5.625 3.938 N Nek4 n/a
9 TRCN0000054768 CGGTCTGCTCTACATTGTCAT pLKO.1 1377 CDS 100% 4.950 3.465 N Nek4 n/a
10 TRCN0000054769 GCTTTGCATGTCATGGGTGAA pLKO.1 2098 CDS 100% 4.050 2.835 N Nek4 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011245068.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14849 pDONR223 64.5% 72.8% 25.4% None (many diffs) n/a
2 ccsbBroad304_14849 pLX_304 0% 72.8% 25.4% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000471117 GTAGCGTTGCACTCCATGGACCCG pLX_317 19.8% 72.8% 25.4% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV