Construct: ORF TRCN0000471117
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018933.3_s317c1
- Derived from:
- ccsbBroadEn_14849
- DNA Barcode:
- GTAGCGTTGCACTCCATGGACCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NEK4 (6787)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471117
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001348413.2 | 95% | 29.9% | (many diffs) |
| 2 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001348412.2 | 92.7% | 29.4% | (many diffs) |
| 3 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_017007086.1 | 88.7% | 28.3% | (many diffs) |
| 4 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_003157.6 | 87.6% | 27.8% | (many diffs) |
| 5 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_011534039.3 | 87.4% | 27.7% | (many diffs) |
| 6 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_017007085.1 | 84.1% | 26.8% | (many diffs) |
| 7 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001348414.2 | 77.9% | 18% | (many diffs) |
| 8 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_011534040.3 | 77.3% | 34.8% | (many diffs) |
| 9 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001193533.2 | 77.1% | 17.2% | (many diffs) |
| 10 | mouse | 23955 | Nek4 | NIMA (never in mitosis gene... | NM_011849.3 | 75.5% | 28.5% | (many diffs) |
| 11 | mouse | 23955 | Nek4 | NIMA (never in mitosis gene... | XM_011245068.2 | 72.8% | 25.4% | (many diffs) |
| 12 | mouse | 23955 | Nek4 | NIMA (never in mitosis gene... | NM_001308328.1 | 70.8% | 30.3% | (many diffs) |
| 13 | mouse | 23955 | Nek4 | NIMA (never in mitosis gene... | XM_011245069.2 | 66.3% | 18.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 807
- ORF length:
- 738
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccctggcc gcctactgct acctgcgggt cgtgggcaag gggagctatg 121 gagaggtgac gcttgtgaag caccggcggg acggcaagca gtatgtcatc aaaaaactga 181 acctccgaaa tgcctctagc cgagagcggc gagctgctga acaggaagcc cagctcttgt 241 ctcagttgaa gcatcccaac attgtcacct acaaggagtc atgggaagga ggagatggtc 301 tgctctacat tgtcatgggc ttctgtgaag gaggtgattt gtaccgaaag ctcaaggagc 361 agaaagggca gcttctgccg gagaatcagg tggtagagtg gtttgtacag atcgccatgg 421 ctttgcagta tttacatgaa aaacacatcc ttcatcgaga tctgaaaact caaaatgtct 481 tcctaacaag aacaaacatc atcaaagtag gggacctagg aattgcccga gtgttggaga 541 accactgtga catggctagc accctcattg gcacacccta ctacatgagc cctgaattgt 601 tctcaaacaa accctacaac tataagtctg atgtttgggc tctaggatgc tgtgtctatg 661 aaatggccac cttgaagcat gctttcaatg caaaagatat gaattcttta gtttatcgga 721 ttattgaagg aaagctgcca gcaatgccaa gagattacag cccagagctg gcaggacctg 781 ataagaacaa tgctgagcaa aaggcctgaa gaaaggccgt ctgtgaggag catcctgagg 841 cagccttata taaagcggca aatctccttc tttttggagg ccacaaagat aaaaacctcc 901 aaaaataaca ttaaaaatgg tgactctcaa tccaagcctt ttgctacagt ggtttctgga 961 gaggcagaat caaatcatga agtaatccac ccccaaccac tctcttctga gggctcccag 1021 acatatataa tgggtgaagg caaatgtttg tcccaggaga aacccagggc ctctggtctc 1081 ttgaagtcac ctgccagtct gaaagcccat acctgcaaac aggacttgag caataccaca 1141 gaactagcca caatcagtag cgtaaatatt gacatcttac ctgcaaaagg gagggattca 1201 gtgagtgatg gctttgttca ggagaatcag ccaagatatt tggatgcctc taatgagtta 1261 ggaggtatat gcagtatttc tcaagtggaa gaggagatgc tgcaggacaa cactaaatcc 1321 agtgcccagc ctgaaaacct gattcccatg tggtcctctg acattgtcac tggggaaaag 1381 aatgaaccag tgaagcctct gcagccccta atcaaagaac aaaagccaaa ggaccaggat 1441 caagttgctg gtgaatgtat tatagaaaaa cagggcagaa tccacccaga tttacagcca 1501 cacaactctg ggtctgaacc ttccctgtct cgacagcgac ggcaaaagag gagagaacag 1561 actgagcaca gaggggaaaa gagacaggtc cgcagagatc tctttgcttt ccaagagtcg 1621 cctcctcgat ttttgccttc tcatcccatt gttgggaaag tggatgtcac atcaacacaa 1681 aaagaggctg aaaaccaacg tagagtggtc actgggtctg tgagcagttc aaggagcagt 1741 gagatgtcat catcaaagga tcgaccatta tcagccagag agaggaggcg actaaagcag 1801 tcacaggaag aaatgtcctc ttcaggaaag gaaacagatt cattgtctgt ctgaggatga 1861 gttaagTTCT TCTACAAGTT CAACTGATAA GTCAGATGGG GATTACGGGG AAGGGAAAGG 1921 TCAGACAAAT GAAATTAATG CCTTGGTACA ATTGATGACT CAGACCCTGA AACTGGATTC 1981 TAAAGAGAGC TGTGAAGATG TCCCGGTAGC AAACCCAGTG TCAGAATTCA AACTTCATCG 2041 GAAATATCGG GACACACTGA TACTTCATGG GAAGGTTGCA GAAGAGGCAG AGGAAATCCA 2101 TTTTAAAGAG CTACCTTCAG CTATTATGCC AGGTTCTGAA AAGATCAGGA GACTAGTTGA 2161 AGTCTTGAGA ACTGATGTAA TTCGTGGCCT GGGAGTTCAG CTTTTAGAGC AGGTGTATGA 2221 TCTTTTGGAG GAGGAGGATG AATTTGATAG AGAGGTGAGT GTCTCATTAA CTGTATCAAG 2281 ATGCTTATGT TATAGAATTT TTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 2341 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 2401 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT AGCGTTGCAC 2461 TCCATGGACC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 2521 tt