Construct: ORF ccsbBroadEn_14849
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018933.1_s300c1, BRDN0000458229
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NEK4 (6787)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001348413.2 | 95% | 29.9% | (many diffs) |
| 2 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001348412.2 | 92.7% | 29.4% | (many diffs) |
| 3 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_017007086.1 | 88.7% | 28.3% | (many diffs) |
| 4 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_003157.6 | 87.6% | 27.8% | (many diffs) |
| 5 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_011534039.3 | 87.4% | 27.7% | (many diffs) |
| 6 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_017007085.1 | 84.1% | 26.8% | (many diffs) |
| 7 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001348414.2 | 77.9% | 18% | (many diffs) |
| 8 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_011534040.3 | 77.3% | 34.8% | (many diffs) |
| 9 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001193533.2 | 77.1% | 17.2% | (many diffs) |
| 10 | mouse | 23955 | Nek4 | NIMA (never in mitosis gene... | NM_011849.3 | 75.5% | 28.5% | (many diffs) |
| 11 | mouse | 23955 | Nek4 | NIMA (never in mitosis gene... | XM_011245068.2 | 72.8% | 25.4% | (many diffs) |
| 12 | mouse | 23955 | Nek4 | NIMA (never in mitosis gene... | NM_001308328.1 | 70.8% | 30.3% | (many diffs) |
| 13 | mouse | 23955 | Nek4 | NIMA (never in mitosis gene... | XM_011245069.2 | 66.3% | 18.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 91
- ORF end:
- 829
- ORF length:
- 738
- Sequence:
-
1 CTTTTTTGAC TGATAGTGAC CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA 61 ATGCCAACTT TGTACAAAAA AGTTGGCACC ATGCCCCTGG CCGCCTACTG CTACCTGCGG 121 GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG ACGCTTGTGA AGCACCGGCG GGACGGCAAG 181 CAGTATGTCA TCAAAAAACT GAACCTCCGA AATGCCTCTA GCCGAGAGCG GCGAGCTGCT 241 GAACAGGAAG CCCAGCTCTT GTCTCAGTTG AAGCATCCCA ACATTGTCAC CTACAAGGAG 301 TCATGGGAAG GAGGAGATGG TCTGCTCTAC ATTGTCATGG GCTTCTGTGA AGGAGGTGAT 361 TTGTACCGAA AGCTCAAGGA GCAGAAAGGG CAGCTTCTGC CGGAGAATCA GGTGGTAGAG 421 TGGTTTGTAC AGATCGCCAT GGCTTTGCAG TATTTACATG AAAAACACAT CCTTCATCGA 481 GATCTGAAAA CTCAAAATGT CTTCCTAACA AGAACAAACA TCATCAAAGT AGGGGACCTA 541 GGAATTGCCC GAGTGTTGGA GAACCACTGT GACATGGCTA GCACCCTCAT TGGCACACCC 601 TACTACATGA GCCCTGAATT GTTCTCAAAC AAACCCTACA ACTATAAGTC TGATGTTTGG 661 GCTCTAGGAT GCTGTGTCTA TGAAATGGCC ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT 721 ATGAATTCTT TAGTTTATCG GATTATTGAA GGAAAGCTGC CAGCAATGCC AAGAGATTAC 781 AGCCCAGAGC TGGCAGGACC TGATaagaac aatgctgagc aaaaggcctg aagaaaggcc 841 gtctgtgagg agcatcctga ggcagcctta tataaagcgg caaatctcct tctttttgga 901 ggccacaaag ataaaaacct ccaaaaataa cattaaaaat ggtgactctc aatccaagcc 961 ttttgctaca gtggtttctg gagaggcaga atcaaatcat gaagtaatcc acccccaacc 1021 actctcttct gagggctccc agacatatat aatgggtgaa ggcaaatgtt tgtcccagga 1081 gaaacccagg gcctctggtc tcttgaagtc acctgccagt ctgaaagccc atacctgcaa 1141 acaggacttg agcaatacca cagaactagc cacaatcagt agcgtaaata ttgacatctt 1201 acctgcaaaa gggagggatt cagtgagtga tggctttgtt caggagaatc agccaagata 1261 tttggatgcc tctaatgagt taggaggtat atgcagtatt tctcaagtgg aagaggagat 1321 gctgcaggac aacactaaat ccagtgccca gcctgaaaac ctgattccca tgtggtcctc 1381 tgacattgtc actggggaaa agaatgaacc agtgaagcct ctgcagcccc taatcaaaga 1441 acaaaagcca aaggaccagg atcaagttgc tggtgaatgt attatagaaa aacagggcag 1501 aatccaccca gatttacagc cacacaactc tgggtctgaa ccttccctgt ctcgacagcg 1561 acggcaaaag aggagagaac agactgagca cagaggggaa aagAGACAGG TCCGCAGAGA 1621 TCTCTTTGCT TTCCAAGAGT CGCCTCCTCG ATTTTTGCCT TCTCATCCCA TTGTTGGGAA 1681 AGTGGATGTC ACATCAACAC AAAAAGAGGC TGAAAACCAA CGTAGAGTGG TCACTGGGTC 1741 TGTGAGCAGT TCAAGGAGCA GTGAGATGTC ATCATCAAAG GATCGACCAT TATCAGCCAG 1801 AGAGAGGAGG CGACTAAAGC AGTCACAGGA AGAAATGTCC TCTTCAGGAA AGGAAACAGA 1861 TTCATTGTCT GTCTGAGGAT GAGTTAAGTT CTTCTACAAG TTCAACTGAT AAGTCAGATG 1921 GGGATTACGG GGAAGGGAAA GGTCAGACAA ATGAAATTAA TGCCTTGGTA CAATTGATGA 1981 CTCAGACCCT GAAACTGGAT TCTAAAGAGA GCTGTGAAGA TGTCCCGGTA GCAAACCCAG 2041 TGTCAGAATT CAAACTTCAT CGGAAATATC GGGACACACT GATACTTCAT GGGAAGGTTG 2101 CAGAAGAGGC AGAGGAAATC CATTTTAAAG AGCTACCTTC AGCTATTATG CCAGGTTCTG 2161 AAAAGATCAG GAGACTAGTT GAAGTCTTGA GAACTGATGT AATTCGTGGC CTGGGAGTTC 2221 AGCTTTTAGA GCAGGTGTAT GATCTTTTGG AGGAGGAGGA TGAATTTGAT AGAGAGGTGA 2281 GTGTCTCATT AACTGTATCA AGATGCTTAT GTTATAGAAT TTTTTTGCCA ACTTTCTTGT 2341 ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA 2401 TCAGTCAAAA TAAAATCATT ATTGCCATCC AGCTATCCCC GC