Construct: ORF ccsbBroadEn_14849

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018933.1_s300c1, BRDN0000458229
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
NEK4 (6787)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6787 NEK4 NIMA related kinase 4 NM_001348413.2 95% 29.9% (many diffs)
2 human 6787 NEK4 NIMA related kinase 4 NM_001348412.2 92.7% 29.4% (many diffs)
3 human 6787 NEK4 NIMA related kinase 4 XM_017007086.1 88.7% 28.3% (many diffs)
4 human 6787 NEK4 NIMA related kinase 4 NM_003157.6 87.6% 27.8% (many diffs)
5 human 6787 NEK4 NIMA related kinase 4 XM_011534039.3 87.4% 27.7% (many diffs)
6 human 6787 NEK4 NIMA related kinase 4 XM_017007085.1 84.1% 26.8% (many diffs)
7 human 6787 NEK4 NIMA related kinase 4 NM_001348414.2 77.9% 18% (many diffs)
8 human 6787 NEK4 NIMA related kinase 4 XM_011534040.3 77.3% 34.8% (many diffs)
9 human 6787 NEK4 NIMA related kinase 4 NM_001193533.2 77.1% 17.2% (many diffs)
10 mouse 23955 Nek4 NIMA (never in mitosis gene... NM_011849.3 75.5% 28.5% (many diffs)
11 mouse 23955 Nek4 NIMA (never in mitosis gene... XM_011245068.2 72.8% 25.4% (many diffs)
12 mouse 23955 Nek4 NIMA (never in mitosis gene... NM_001308328.1 70.8% 30.3% (many diffs)
13 mouse 23955 Nek4 NIMA (never in mitosis gene... XM_011245069.2 66.3% 18.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
91
ORF end:
829
ORF length:
738
Sequence:
1CTTTTTTGAC TGATAGTGAC CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA
61ATGCCAACTT TGTACAAAAA AGTTGGCACC ATGCCCCTGG CCGCCTACTG CTACCTGCGG
121GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG ACGCTTGTGA AGCACCGGCG GGACGGCAAG
181CAGTATGTCA TCAAAAAACT GAACCTCCGA AATGCCTCTA GCCGAGAGCG GCGAGCTGCT
241GAACAGGAAG CCCAGCTCTT GTCTCAGTTG AAGCATCCCA ACATTGTCAC CTACAAGGAG
301TCATGGGAAG GAGGAGATGG TCTGCTCTAC ATTGTCATGG GCTTCTGTGA AGGAGGTGAT
361TTGTACCGAA AGCTCAAGGA GCAGAAAGGG CAGCTTCTGC CGGAGAATCA GGTGGTAGAG
421TGGTTTGTAC AGATCGCCAT GGCTTTGCAG TATTTACATG AAAAACACAT CCTTCATCGA
481GATCTGAAAA CTCAAAATGT CTTCCTAACA AGAACAAACA TCATCAAAGT AGGGGACCTA
541GGAATTGCCC GAGTGTTGGA GAACCACTGT GACATGGCTA GCACCCTCAT TGGCACACCC
601TACTACATGA GCCCTGAATT GTTCTCAAAC AAACCCTACA ACTATAAGTC TGATGTTTGG
661GCTCTAGGAT GCTGTGTCTA TGAAATGGCC ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT
721ATGAATTCTT TAGTTTATCG GATTATTGAA GGAAAGCTGC CAGCAATGCC AAGAGATTAC
781AGCCCAGAGC TGGCAGGACC TGATaagaac aatgctgagc aaaaggcctg aagaaaggcc
841gtctgtgagg agcatcctga ggcagcctta tataaagcgg caaatctcct tctttttgga
901ggccacaaag ataaaaacct ccaaaaataa cattaaaaat ggtgactctc aatccaagcc
961ttttgctaca gtggtttctg gagaggcaga atcaaatcat gaagtaatcc acccccaacc
1021actctcttct gagggctccc agacatatat aatgggtgaa ggcaaatgtt tgtcccagga
1081gaaacccagg gcctctggtc tcttgaagtc acctgccagt ctgaaagccc atacctgcaa
1141acaggacttg agcaatacca cagaactagc cacaatcagt agcgtaaata ttgacatctt
1201acctgcaaaa gggagggatt cagtgagtga tggctttgtt caggagaatc agccaagata
1261tttggatgcc tctaatgagt taggaggtat atgcagtatt tctcaagtgg aagaggagat
1321gctgcaggac aacactaaat ccagtgccca gcctgaaaac ctgattccca tgtggtcctc
1381tgacattgtc actggggaaa agaatgaacc agtgaagcct ctgcagcccc taatcaaaga
1441acaaaagcca aaggaccagg atcaagttgc tggtgaatgt attatagaaa aacagggcag
1501aatccaccca gatttacagc cacacaactc tgggtctgaa ccttccctgt ctcgacagcg
1561acggcaaaag aggagagaac agactgagca cagaggggaa aagAGACAGG TCCGCAGAGA
1621TCTCTTTGCT TTCCAAGAGT CGCCTCCTCG ATTTTTGCCT TCTCATCCCA TTGTTGGGAA
1681AGTGGATGTC ACATCAACAC AAAAAGAGGC TGAAAACCAA CGTAGAGTGG TCACTGGGTC
1741TGTGAGCAGT TCAAGGAGCA GTGAGATGTC ATCATCAAAG GATCGACCAT TATCAGCCAG
1801AGAGAGGAGG CGACTAAAGC AGTCACAGGA AGAAATGTCC TCTTCAGGAA AGGAAACAGA
1861TTCATTGTCT GTCTGAGGAT GAGTTAAGTT CTTCTACAAG TTCAACTGAT AAGTCAGATG
1921GGGATTACGG GGAAGGGAAA GGTCAGACAA ATGAAATTAA TGCCTTGGTA CAATTGATGA
1981CTCAGACCCT GAAACTGGAT TCTAAAGAGA GCTGTGAAGA TGTCCCGGTA GCAAACCCAG
2041TGTCAGAATT CAAACTTCAT CGGAAATATC GGGACACACT GATACTTCAT GGGAAGGTTG
2101CAGAAGAGGC AGAGGAAATC CATTTTAAAG AGCTACCTTC AGCTATTATG CCAGGTTCTG
2161AAAAGATCAG GAGACTAGTT GAAGTCTTGA GAACTGATGT AATTCGTGGC CTGGGAGTTC
2221AGCTTTTAGA GCAGGTGTAT GATCTTTTGG AGGAGGAGGA TGAATTTGAT AGAGAGGTGA
2281GTGTCTCATT AACTGTATCA AGATGCTTAT GTTATAGAAT TTTTTTGCCA ACTTTCTTGT
2341ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA
2401TCAGTCAAAA TAAAATCATT ATTGCCATCC AGCTATCCCC GC

Download FASTA (ORF) (Full)

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