Transcript: Mouse XM_011247129.2

PREDICTED: Mus musculus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Chuk (12675)
Length:
2397
CDS:
116..2389

Additional Resources:

NCBI RefSeq record:
XM_011247129.2
NBCI Gene record:
Chuk (12675)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011247129.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000012352 GCGTGCCATTGATCTCTATAA pLKO.1 1801 CDS 100% 13.200 18.480 N Chuk n/a
2 TRCN0000297778 GCGTGCCATTGATCTCTATAA pLKO_005 1801 CDS 100% 13.200 18.480 N Chuk n/a
3 TRCN0000244901 TGAATGTATTGCTGGATATAG pLKO_005 751 CDS 100% 13.200 10.560 N CHUK n/a
4 TRCN0000012350 GCAGCAATGTTAAGTCTTCTT pLKO.1 1463 CDS 100% 4.950 3.960 N Chuk n/a
5 TRCN0000280138 GCAGCAATGTTAAGTCTTCTT pLKO_005 1463 CDS 100% 4.950 3.960 N Chuk n/a
6 TRCN0000012351 GCTGACAATACTGTCATGTTT pLKO.1 2018 CDS 100% 0.563 0.394 N Chuk n/a
7 TRCN0000280139 GCTGACAATACTGTCATGTTT pLKO_005 2018 CDS 100% 0.563 0.394 N Chuk n/a
8 TRCN0000012349 CCTAAATATGACTTCTGCAAA pLKO.1 1060 CDS 100% 4.950 2.970 N Chuk n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011247129.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489445 CATGAACCATAATCTGTGACGGGA pLX_317 16% 86.4% 88.4% V5 (many diffs) n/a
2 TRCN0000488536 TGGCAAGATATCCTGAGACTAGCT pLX_317 16% 86.3% 88.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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