Transcript: Human XM_011513452.2

PREDICTED: Homo sapiens G protein-coupled receptor kinase 4 (GRK4), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GRK4 (2868)
Length:
2605
CDS:
568..2265

Additional Resources:

NCBI RefSeq record:
XM_011513452.2
NBCI Gene record:
GRK4 (2868)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145617 TGCCCATAACTACCTAAGAG pXPR_003 GGG 463 27% 6 0.5307 GRK4 GRK4 77725
2 BRDN0001146308 GTTGGAACAGTCGGCTACAT pXPR_003 GGG 1172 69% 12 0.504 GRK4 GRK4 77724
3 BRDN0001145215 GCGGCTTGGAAGATTTACAG pXPR_003 AGG 1026 60% 10 0.2411 GRK4 GRK4 77723
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011513452.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196375 GCTTTGCCATTAGATCTAGAC pLKO.1 2143 CDS 100% 4.050 5.670 N GRK4 n/a
2 TRCN0000000988 ACCCGTAACAAAGAACACATT pLKO.1 1107 CDS 100% 4.950 3.960 N GRK4 n/a
3 TRCN0000196675 GAATACCAAGAAAGCTCATAT pLKO.1 1048 CDS 100% 13.200 9.240 N GRK4 n/a
4 TRCN0000196505 GCAAAGTAGATTCGTAGTTAG pLKO.1 1410 CDS 100% 10.800 7.560 N GRK4 n/a
5 TRCN0000000990 CTTGGCTGTCTGATCTATGAA pLKO.1 1798 CDS 100% 5.625 3.938 N GRK4 n/a
6 TRCN0000000989 GAATATGAAGTTGCCGATGAT pLKO.1 832 CDS 100% 4.950 3.465 N GRK4 n/a
7 TRCN0000000986 GCCTACGCTTATGAAACCAAA pLKO.1 1435 CDS 100% 4.950 3.465 N GRK4 n/a
8 TRCN0000196888 GCTTCTTCTATAGACTCTTCA pLKO.1 2201 CDS 100% 4.950 3.465 N GRK4 n/a
9 TRCN0000197064 GCAGTCTTTGTGACAAGCAAC pLKO.1 728 CDS 100% 4.050 2.835 N GRK4 n/a
10 TRCN0000194891 CTGTCAATCTTAGATAGATTC pLKO.1 874 CDS 100% 1.080 0.756 N GRK4 n/a
11 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 2559 3UTR 100% 4.950 2.475 Y ERAP2 n/a
12 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2560 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011513452.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06321 pDONR223 100% 83.5% 82.2% None (many diffs) n/a
2 ccsbBroad304_06321 pLX_304 0% 83.5% 82.2% V5 (many diffs) n/a
3 TRCN0000491646 ATACAACCCAACAAAGTTTTTTTC pLX_317 16.5% 83.5% 82.2% V5 (many diffs) n/a
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