Construct: ORF ccsbBroad304_06321

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004239.1_s304c1, BRDN0000411744
Derived from:
ccsbBroadEn_06321
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRK4 (2868)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06321
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2868 GRK4 G protein-coupled receptor ... NM_182982.3 99.9% 99.8% 1457T>C
2 human 2868 GRK4 G protein-coupled receptor ... NM_001004056.2 94.4% 94.2% 51_52ins96;1361T>C
3 human 2868 GRK4 G protein-coupled receptor ... XM_011513447.2 93.6% 93.5% 601_717del;1574T>C
4 human 2868 GRK4 G protein-coupled receptor ... NM_001004057.2 91.9% 91.8% 1457T>C;1544_1545ins138
5 human 2868 GRK4 G protein-coupled receptor ... XM_011513448.2 90.9% 89.6% (many diffs)
6 human 2868 GRK4 G protein-coupled receptor ... XM_017008052.1 89.4% 89.3% 260_261ins78;523_639del;1496T>C
7 human 2868 GRK4 G protein-coupled receptor ... XM_017008053.1 88.7% 88.6% 601_717del;1086_1087ins90;1484T>C
8 human 2868 GRK4 G protein-coupled receptor ... XM_011513449.2 88.4% 88.3% 51_52ins96;505_621del;1478T>C
9 human 2868 GRK4 G protein-coupled receptor ... NM_005307.3 86.4% 86.3% 51_52ins96;1361T>C;1448_1449ins138
10 human 2868 GRK4 G protein-coupled receptor ... XM_011513450.2 86.1% 86% 601_717del;1574T>C;1661_1662ins138
11 human 2868 GRK4 G protein-coupled receptor ... XM_011513451.2 86.1% 86% 601_717del;1386_1387ins138;1436T>C
12 human 2868 GRK4 G protein-coupled receptor ... XM_017008054.1 84% 83.9% 1269_1270ins138;1319T>C;1406_1407ins138
13 human 2868 GRK4 G protein-coupled receptor ... XM_011513452.2 83.5% 82.2% (many diffs)
14 human 2868 GRK4 G protein-coupled receptor ... XM_011513453.2 78.7% 78.7% 601_717del;1524_1525ins276
15 human 2868 GRK4 G protein-coupled receptor ... XM_011513454.2 78.7% 78.6% (many diffs)
16 human 2868 GRK4 G protein-coupled receptor ... XM_017008055.1 78.4% 78.3% (many diffs)
17 human 2868 GRK4 G protein-coupled receptor ... XM_017008056.1 73.3% 76.1% (many diffs)
18 human 2868 GRK4 G protein-coupled receptor ... XM_011513455.2 68.7% 71.3% (many diffs)
19 human 2868 GRK4 G protein-coupled receptor ... NM_001350173.2 66.3% 65.3% 0_1ins518;16_17ins64;875T>C
20 human 2868 GRK4 G protein-coupled receptor ... XM_017008057.1 66.3% 65.3% 0_1ins518;16_17ins64;875T>C
21 human 2868 GRK4 G protein-coupled receptor ... XM_017008058.1 66.3% 65.3% 0_1ins518;16_17ins64;875T>C
22 human 2868 GRK4 G protein-coupled receptor ... XM_017008059.2 66.3% 65.3% 0_1ins518;16_17ins64;875T>C
23 human 2868 GRK4 G protein-coupled receptor ... XM_024454015.1 66.3% 65.3% 0_1ins518;16_17ins64;875T>C
24 human 2868 GRK4 G protein-coupled receptor ... XM_005247962.3 63.4% 63.3% 0_1ins633;824T>C
25 human 2868 GRK4 G protein-coupled receptor ... XM_006713880.3 63.4% 63.3% 0_1ins633;824T>C
26 human 2868 GRK4 G protein-coupled receptor ... XM_017008062.1 63.4% 63.3% 0_1ins633;824T>C
27 human 2868 GRK4 G protein-coupled receptor ... XR_924941.2 58.7% (many diffs)
28 human 2868 GRK4 G protein-coupled receptor ... XM_017008063.1 58.4% 57.4% (many diffs)
29 human 2868 GRK4 G protein-coupled receptor ... XM_017008064.1 58.4% 57.4% (many diffs)
30 human 2868 GRK4 G protein-coupled receptor ... XR_001741211.1 55.2% (many diffs)
31 human 2868 GRK4 G protein-coupled receptor ... XM_011513456.2 53.2% 52.9% (many diffs)
32 human 2868 GRK4 G protein-coupled receptor ... XM_017008066.1 50.4% 49.4% (many diffs)
33 human 2868 GRK4 G protein-coupled receptor ... XR_924943.2 49.7% (many diffs)
34 human 2868 GRK4 G protein-coupled receptor ... XR_001741210.1 43.8% (many diffs)
35 human 2868 GRK4 G protein-coupled receptor ... XM_011513457.2 42.3% 40.8% (many diffs)
36 human 2868 GRK4 G protein-coupled receptor ... XM_017008065.1 40.5% 40.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1803
ORF length:
1734
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggagctcgag aacatcgtgg ccaactcgct gctgctgaaa gcgcgtcaag
121gaggatatgg caaaaaaagt ggtcgtagta aaaaatggaa ggagatactg acactgcctc
181ctgtcagcca gtgcagtgag cttagacatt ccattgaaaa ggattatagc agtctttgtg
241acaagcaacc gataggaaga cgtctcttca ggcagttctg tgataccaaa cccactctaa
301agaggcacat tgaattcttg gatgcagtgg cagaatatga agttgccgat gatgaggacc
361gaagtgattg tggactgtca atcttagata gattcttcaa tgataagttg gcagcccctt
421taccagaaat acctccagat gttgtgacag aatgtagatt gggactgaag gaggagaacc
481cttccaaaaa agcctttgag gaatgtacta gagttgccca taactaccta agaggggaac
541catttgaaga ataccaagaa agctcatatt tttctcagtt tttacaatgg aaatggctgg
601aaaggcaacc cgtaacaaag aacacattta gacattacag agttctagga aaaggcggat
661ttggagaggt ttgcgcctgt caagtgcgag ccacaggaaa aatgtatgcc tgcaaaaagc
721tacaaaaaaa aagaataaag aagaggaaag gtgaagctat ggctctaaat gagaaaagaa
781ttctggagaa agtgcaaagt agattcgtag ttagtttagc ctacgcttat gaaaccaaag
841atgccttgtg cttggtgctc accattatga atggagggga tttgaagttt cacatttaca
901acctgggcaa tcccggcttt gatgagcaga gagccgtttt ctatgctgca gagctgtgtt
961gcggcttgga agatttacag agggaaagaa ttgtatacag agacttgaag cctgagaata
1021ttctccttga tgatcgtgga cacatccgga tttcagacct cggtttggcc acagagatcc
1081cagaaggaca gagggttcga ggaagagttg gaacagtcgg ctacatggca cctgaagttg
1141tcaataatga aaagtatacg tttagtcccg attggtgggg acttggctgt ctgatctatg
1201aaatgattca gggacattct ccattcaaaa aatacaaaga gaaagtcaaa tgggaggagg
1261tcgatcaaag aatcaagaat gataccgagg agtattctga gaagttttca gaggatgcca
1321aatctatctg caggatgtta ctcaccaaga atccaagcaa gcggctgggc tgcaggggcg
1381agggagcggc tggggtgaag cagcaccccg tgttcaagga catcaacttc aggaggctgg
1441aggcaaacat gctggagccc cctttctgtc ctgatcctca tgccgtttac tgtaaggacg
1501tcctggatat cgagcagttc tcggcggtga aagggatcta cctggacacc gcagatgaag
1561acttctatgc tcggtttgct accgggtgtg tctccatccc ctggcagaat gagatgatcg
1621aatctgggtg tttcaaagac atcaacaaaa gtgaaagtga ggaagctttg ccattagatc
1681tagacaagaa catacatacc ccggtttcca gaccaaacag aggcttcttc tatagactct
1741tcagaagagg gggctgcctg accatggtcc ccagtgagaa ggaagtggaa cccaagcaat
1801gcttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1861tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
1921tctggatta

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