Transcript: Human XM_011516427.1

PREDICTED: Homo sapiens actin related protein 3B (ACTR3B), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ACTR3B (57180)
Length:
1091
CDS:
117..1076

Additional Resources:

NCBI RefSeq record:
XM_011516427.1
NBCI Gene record:
ACTR3B (57180)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011516427.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000108328 GCCATCGATAAACCTACATAT pLKO.1 312 CDS 100% 13.200 6.600 Y ACTR3B n/a
2 TRCN0000418383 CGATACGACATGGAATCATTG pLKO_005 346 CDS 100% 10.800 5.400 Y ACTR3B n/a
3 TRCN0000434511 TCATTGAAGACTGGGATCTTA pLKO_005 361 CDS 100% 5.625 2.813 Y ACTR3B n/a
4 TRCN0000414318 AGTTCATTATTCCTTCATGTA pLKO_005 199 CDS 100% 4.950 2.475 Y ACTR3B n/a
5 TRCN0000423092 TAGGAGATGAAGCCATCGATA pLKO_005 301 CDS 100% 4.950 2.475 Y ACTR3B n/a
6 TRCN0000108327 CCAGGACTCTACATTGCAGTT pLKO.1 525 CDS 100% 4.050 2.025 Y ACTR3B n/a
7 TRCN0000414906 GAGTCAGCAAAGGTAGTTGAC pLKO_005 231 CDS 100% 4.050 2.025 Y ACTR3B n/a
8 TRCN0000090296 CCAGGACTCTACATTGCAGTA pLKO.1 525 CDS 100% 4.050 2.025 Y Actr3b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011516427.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03802 pDONR223 100% 91% 91% None 952_954delATT;956G>C;957_958ins90 n/a
2 ccsbBroad304_03802 pLX_304 0% 91% 91% V5 952_954delATT;956G>C;957_958ins90 n/a
3 TRCN0000491360 AAGTAAGAACTACCAGACTCACCT pLX_317 33.1% 91% 91% V5 952_954delATT;956G>C;957_958ins90 n/a
4 ccsbBroadEn_03803 pDONR223 100% 76% 75.8% None 953T>A;955_956delCGinsGT;957_958ins297 n/a
5 ccsbBroad304_03803 pLX_304 0% 76% 75.8% V5 953T>A;955_956delCGinsGT;957_958ins297 n/a
6 TRCN0000480353 AATTTTCGGGTCTCCGCCAGAGAG pLX_317 33.6% 76% 75.8% V5 953T>A;955_956delCGinsGT;957_958ins297 n/a
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