Construct: ORF ccsbBroadEn_03803
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008885.1_s300c1, BRDN0000389568
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTR3B (57180)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57180 | ACTR3B | actin related protein 3B | NM_020445.6 | 100% | 100% | |
| 2 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350944.1 | 89.9% | 89.9% | 948_949ins126 |
| 3 | human | 57180 | ACTR3B | actin related protein 3B | XM_017012460.1 | 83.8% | 74.9% | (many diffs) |
| 4 | human | 57180 | ACTR3B | actin related protein 3B | XM_006716065.2 | 83.3% | 78.7% | (many diffs) |
| 5 | human | 57180 | ACTR3B | actin related protein 3B | NM_001040135.2 | 83.2% | 83.2% | 951_952ins210 |
| 6 | human | 57180 | ACTR3B | actin related protein 3B | XM_006716066.1 | 81.1% | 73.9% | (many diffs) |
| 7 | human | 57180 | ACTR3B | actin related protein 3B | XM_011516425.2 | 79.7% | 75.2% | (many diffs) |
| 8 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350940.1 | 78.9% | 78.9% | 0_1ins264 |
| 9 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350941.1 | 78.9% | 78.9% | 0_1ins264 |
| 10 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350942.1 | 78.9% | 78.9% | 0_1ins264 |
| 11 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350943.1 | 78.9% | 78.9% | 0_1ins264 |
| 12 | human | 57180 | ACTR3B | actin related protein 3B | XM_006716067.4 | 77.1% | 76.3% | (many diffs) |
| 13 | human | 57180 | ACTR3B | actin related protein 3B | XM_011516426.2 | 77% | 76.5% | (many diffs) |
| 14 | human | 10096 | ACTR3 | actin related protein 3 | NM_005721.5 | 76.8% | 90.6% | (many diffs) |
| 15 | human | 57180 | ACTR3B | actin related protein 3B | XM_011516427.1 | 76% | 75.8% | 953T>A;955_956delCGinsGT;957_958ins297 |
| 16 | human | 57180 | ACTR3B | actin related protein 3B | XM_017012461.1 | 75.8% | 75.8% | 951_952ins303 |
| 17 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350945.1 | 69.1% | 69.1% | 0_1ins387 |
| 18 | human | 57180 | ACTR3B | actin related protein 3B | XM_017012463.1 | 69.1% | 69.1% | 0_1ins387 |
| 19 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350946.1 | 62.2% | 62.2% | 0_1ins264;687_688ins210 |
| 20 | human | 57180 | ACTR3B | actin related protein 3B | NR_146951.1 | 56.3% | 1_134del;673_674insGGCA;1385_2213del | |
| 21 | human | 57180 | ACTR3B | actin related protein 3B | NR_146949.1 | 53.8% | (many diffs) | |
| 22 | human | 653857 | ACTR3C | actin related protein 3C | XM_017012548.1 | 52.6% | 49.6% | (many diffs) |
| 23 | human | 57180 | ACTR3B | actin related protein 3B | XM_017012465.1 | 52.3% | 52.3% | 0_1ins387;564_565ins210 |
| 24 | human | 57180 | ACTR3B | actin related protein 3B | NR_146947.1 | 52.2% | 1_134del;721_722ins95;1294_2122del | |
| 25 | human | 653857 | ACTR3C | actin related protein 3C | XM_011516502.2 | 51.3% | 47% | (many diffs) |
| 26 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446879.1 | 51.3% | 47% | (many diffs) |
| 27 | human | 57180 | ACTR3B | actin related protein 3B | NR_146950.1 | 50.7% | (many diffs) | |
| 28 | human | 57180 | ACTR3B | actin related protein 3B | NR_146948.1 | 49.7% | (many diffs) | |
| 29 | human | 57180 | ACTR3B | actin related protein 3B | NR_146946.1 | 48.9% | (many diffs) | |
| 30 | human | 653857 | ACTR3C | actin related protein 3C | XM_005250043.4 | 46.6% | 44.8% | (many diffs) |
| 31 | human | 653857 | ACTR3C | actin related protein 3C | NM_001164458.1 | 46.5% | 44.7% | (many diffs) |
| 32 | human | 653857 | ACTR3C | actin related protein 3C | NM_001164459.1 | 46.5% | 44.7% | (many diffs) |
| 33 | human | 653857 | ACTR3C | actin related protein 3C | XM_011516506.3 | 46.5% | 44.7% | (many diffs) |
| 34 | human | 653857 | ACTR3C | actin related protein 3C | XM_011516507.2 | 46.5% | 44.7% | (many diffs) |
| 35 | human | 653857 | ACTR3C | actin related protein 3C | XM_011516508.2 | 46.5% | 44.7% | (many diffs) |
| 36 | human | 653857 | ACTR3C | actin related protein 3C | XM_017012549.1 | 46.5% | 44.7% | (many diffs) |
| 37 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446880.1 | 46.5% | 44.7% | (many diffs) |
| 38 | human | 653857 | ACTR3C | actin related protein 3C | XM_011516511.3 | 45.6% | 44.9% | (many diffs) |
| 39 | human | 653857 | ACTR3C | actin related protein 3C | NM_001351027.1 | 27.8% | 25.7% | (many diffs) |
| 40 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446884.1 | 27.8% | 25.7% | (many diffs) |
| 41 | human | 653857 | ACTR3C | actin related protein 3C | NM_001351028.2 | 26.7% | 25.5% | (many diffs) |
| 42 | human | 653857 | ACTR3C | actin related protein 3C | XM_017012550.2 | 26.7% | 25.5% | (many diffs) |
| 43 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446881.1 | 26.7% | 25.5% | (many diffs) |
| 44 | human | 653857 | ACTR3C | actin related protein 3C | NM_001351029.1 | 14.4% | 11.4% | (many diffs) |
| 45 | human | 653857 | ACTR3C | actin related protein 3C | NM_001351030.1 | 14.4% | 11.4% | (many diffs) |
| 46 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446882.1 | 14.4% | 11.4% | (many diffs) |
| 47 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446883.1 | 14.4% | 11.4% | (many diffs) |
| 48 | mouse | 242894 | Actr3b | ARP3 actin-related protein 3B | NM_001004365.2 | 89.8% | 98.5% | (many diffs) |
| 49 | mouse | 242894 | Actr3b | ARP3 actin-related protein 3B | XM_006535713.3 | 78.6% | 87% | (many diffs) |
| 50 | mouse | 242894 | Actr3b | ARP3 actin-related protein 3B | NM_001310504.1 | 71% | 77.7% | (many diffs) |
| 51 | mouse | 242894 | Actr3b | ARP3 actin-related protein 3B | XM_011249768.2 | 62% | 67.9% | (many diffs) |
| 52 | mouse | 242894 | Actr3b | ARP3 actin-related protein 3B | XM_017320880.1 | 62% | 67.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC AGGCTCCCTG CCTCCCTGCG TGGTGGACTG TGGCACCGGG TATACCAAGC 121 TTGGCTACGC AGGCAACACT GAGCCCCAGT TCATTATTCC TTCATGTATT GCCATCAGAG 181 AGTCAGCAAA GGTAGTTGAC CAAGCTCAAA GGAGAGTGTT GAGGGGAGTT GATGACCTTG 241 ACTTTTTCAT AGGAGATGAA GCCATCGATA AACCTACATA TGCTACAAAG TGGCCGATAC 301 GACATGGAAT CATTGAAGAC TGGGATCTTA TGGAAAGGTT CATGGAGCAA GTGGTTTTTA 361 AATATCTTCG AGCTGAACCT GAGGACCATT ATTTTTTAAT GACAGAACCT CCACTCAATA 421 CACCAGAAAA CAGAGAGTAT CTTGCAGAAA TTATGTTTGA ATCATTTAAC GTACCAGGAC 481 TCTACATTGC AGTTCAGGCA GTGCTGGCCT TGGCGGCATC TTGGACATCT CGACAAGTGG 541 GTGAACGTAC GTTAACGGGG ATAGTCATTG ACAGCGGAGA TGGAGTCACC CATGTTATCC 601 CAGTGGCAGA AGGTTATGTA ATTGGAAGCT GCATCAAACA CATCCCGATT GCAGGTAGAG 661 ATATTACGTA TTTCATTCAA CAGCTGCTAA GGGAGAGGGA GGTGGGAATC CCTCCTGAGC 721 AGTCACTGGA GACCGCAAAA GCCATTAAGG AGAAATACTG TTACATTTGC CCCGATATAG 781 TCAAGGAATT TGCCAAGTAT GATGTGGATC CCCGGAAGTG GATCAAACAG TACACGGGTA 841 TCAATGCGAT CAACCAGAAG AAGTTTGTTA TAGACGTTGG TTACGAAAGA TTCCTGGGAC 901 CTGAAATATT CTTTCACCCG GAGTTTGCCA ACCCAGACTT TATGGAGTCC ATCTCAGATG 961 TTGTTGATGA AGTAATACAG AACTGCCCCA TCGATGTGCG GCGCCCGCTG TATAAGAATG 1021 TCGTACTCTC AGGAGGCTCC ACCATGTTCA GGGATTTCGG ACGCCGACTG CAGAGGGATT 1081 TGAAGAGAGT GGTGGATGCT AGGCTGAGGC TCAGCGAGGA GCTCAGCGGC GGGAGGATCA 1141 AGCCGAAGCC TGTGGAGGTC CAGGTGGTCA CGCATCACAT GCAGCGCTAC GCCGTGTGGT 1201 TCGGAGGCTC CATGCTGGCC TCGACTCCCG AGTTCTTTCA GGTCTGCCAC ACCAAGAAGG 1261 ACTATGAAGA GTACGGGCCC AGCATCTGCC GCCACAACCC CGTCTTTGGA GTCATGTCCT 1321 ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca 1381 acgaac