Construct: ORF TRCN0000491360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016138.1_s317c1
- Derived from:
- ccsbBroadEn_03802
- DNA Barcode:
- AAGTAAGAACTACCAGACTCACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTR3B (57180)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491360
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57180 | ACTR3B | actin related protein 3B | NM_001040135.2 | 100% | 100% | |
| 2 | human | 57180 | ACTR3B | actin related protein 3B | XM_006716066.1 | 94.5% | 92.2% | (many diffs) |
| 3 | human | 57180 | ACTR3B | actin related protein 3B | XM_011516425.2 | 94.4% | 91.2% | (many diffs) |
| 4 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350944.1 | 92.5% | 92.5% | 952_1035del |
| 5 | human | 57180 | ACTR3B | actin related protein 3B | XM_006716067.4 | 92.2% | 91.6% | (many diffs) |
| 6 | human | 57180 | ACTR3B | actin related protein 3B | XM_011516426.2 | 91.1% | 91.3% | (many diffs) |
| 7 | human | 57180 | ACTR3B | actin related protein 3B | XM_017012461.1 | 91% | 91% | 951_952ins93 |
| 8 | human | 57180 | ACTR3B | actin related protein 3B | XM_017012460.1 | 91% | 86.3% | (many diffs) |
| 9 | human | 57180 | ACTR3B | actin related protein 3B | XM_011516427.1 | 91% | 91% | 952_954delATT;956G>C;957_958ins90 |
| 10 | human | 57180 | ACTR3B | actin related protein 3B | XM_006716065.2 | 89.6% | 86.4% | (many diffs) |
| 11 | human | 57180 | ACTR3B | actin related protein 3B | NM_020445.6 | 83.2% | 83.2% | 952_1161del |
| 12 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350946.1 | 74.7% | 74.7% | 0_1ins264 |
| 13 | human | 10096 | ACTR3 | actin related protein 3 | NM_005721.5 | 64.6% | 75.5% | (many diffs) |
| 14 | human | 57180 | ACTR3B | actin related protein 3B | XM_017012465.1 | 62.9% | 62.9% | 0_1ins387 |
| 15 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350940.1 | 62.2% | 62.2% | 0_1ins264;688_897del |
| 16 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350941.1 | 62.2% | 62.2% | 0_1ins264;688_897del |
| 17 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350942.1 | 62.2% | 62.2% | 0_1ins264;688_897del |
| 18 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350943.1 | 62.2% | 62.2% | 0_1ins264;688_897del |
| 19 | human | 653857 | ACTR3C | actin related protein 3C | XM_005250043.4 | 56.1% | 53.8% | (many diffs) |
| 20 | human | 653857 | ACTR3C | actin related protein 3C | NM_001164458.1 | 56% | 54.5% | (many diffs) |
| 21 | human | 653857 | ACTR3C | actin related protein 3C | NM_001164459.1 | 56% | 54.5% | (many diffs) |
| 22 | human | 653857 | ACTR3C | actin related protein 3C | XM_011516506.3 | 56% | 54.5% | (many diffs) |
| 23 | human | 653857 | ACTR3C | actin related protein 3C | XM_011516507.2 | 56% | 54.5% | (many diffs) |
| 24 | human | 653857 | ACTR3C | actin related protein 3C | XM_011516508.2 | 56% | 54.5% | (many diffs) |
| 25 | human | 653857 | ACTR3C | actin related protein 3C | XM_017012549.1 | 56% | 54.5% | (many diffs) |
| 26 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446880.1 | 56% | 54.5% | (many diffs) |
| 27 | human | 653857 | ACTR3C | actin related protein 3C | XM_011516511.3 | 54.6% | 54% | (many diffs) |
| 28 | human | 653857 | ACTR3C | actin related protein 3C | XM_011516502.2 | 53.6% | 48.2% | (many diffs) |
| 29 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446879.1 | 53.6% | 48.2% | (many diffs) |
| 30 | human | 57180 | ACTR3B | actin related protein 3B | NM_001350945.1 | 52.3% | 52.3% | 0_1ins387;565_774del |
| 31 | human | 57180 | ACTR3B | actin related protein 3B | XM_017012463.1 | 52.3% | 52.3% | 0_1ins387;565_774del |
| 32 | human | 399746 | ACTR3BP5 | ACTR3B pseudogene 5 | NR_045000.1 | 51.1% | (many diffs) | |
| 33 | human | 653857 | ACTR3C | actin related protein 3C | XM_017012548.1 | 50.7% | 43.5% | (many diffs) |
| 34 | human | 57180 | ACTR3B | actin related protein 3B | NR_146951.1 | 46.9% | (many diffs) | |
| 35 | human | 57180 | ACTR3B | actin related protein 3B | NR_146949.1 | 44.3% | (many diffs) | |
| 36 | human | 57180 | ACTR3B | actin related protein 3B | NR_146947.1 | 42.8% | (many diffs) | |
| 37 | human | 57180 | ACTR3B | actin related protein 3B | NR_146950.1 | 41.2% | (many diffs) | |
| 38 | human | 57180 | ACTR3B | actin related protein 3B | NR_146948.1 | 40.2% | (many diffs) | |
| 39 | human | 57180 | ACTR3B | actin related protein 3B | NR_146946.1 | 39.6% | (many diffs) | |
| 40 | human | 440888 | ACTR3BP2 | ACTR3B pseudogene 2 | NR_027714.1 | 36.2% | (many diffs) | |
| 41 | human | 653857 | ACTR3C | actin related protein 3C | NM_001351027.1 | 33.3% | 30.8% | (many diffs) |
| 42 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446884.1 | 33.3% | 30.8% | (many diffs) |
| 43 | human | 653857 | ACTR3C | actin related protein 3C | NM_001351028.2 | 32.1% | 30.7% | (many diffs) |
| 44 | human | 653857 | ACTR3C | actin related protein 3C | XM_017012550.2 | 32.1% | 30.7% | (many diffs) |
| 45 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446881.1 | 32.1% | 30.7% | (many diffs) |
| 46 | human | 653857 | ACTR3C | actin related protein 3C | NM_001351029.1 | 17.2% | 13.7% | (many diffs) |
| 47 | human | 653857 | ACTR3C | actin related protein 3C | NM_001351030.1 | 17.2% | 13.7% | (many diffs) |
| 48 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446882.1 | 17.2% | 13.7% | (many diffs) |
| 49 | human | 653857 | ACTR3C | actin related protein 3C | XM_024446883.1 | 17.2% | 13.7% | (many diffs) |
| 50 | human | 653857 | ACTR3C | actin related protein 3C | NR_147013.2 | 8.9% | (many diffs) | |
| 51 | mouse | 242894 | Actr3b | ARP3 actin-related protein 3B | NM_001004365.2 | 75.6% | 82.2% | (many diffs) |
| 52 | mouse | 242894 | Actr3b | ARP3 actin-related protein 3B | XM_006535713.3 | 64.4% | 70.8% | (many diffs) |
| 53 | mouse | 242894 | Actr3b | ARP3 actin-related protein 3B | NM_001310504.1 | 56.8% | 61.4% | (many diffs) |
| 54 | mouse | 242894 | Actr3b | ARP3 actin-related protein 3B | XM_011249768.2 | 47.8% | 51.6% | (many diffs) |
| 55 | mouse | 242894 | Actr3b | ARP3 actin-related protein 3B | XM_017320880.1 | 47.8% | 51.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1110
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggctccctg cctccctgcg tggtggactg tggcaccggg tataccaagc 121 ttggctacgc aggcaacact gagccccagt tcattattcc ttcatgtatt gccatcagag 181 agtcagcaaa ggtagttgac caagctcaaa ggagagtgtt gaggggagtt gatgaccttg 241 actttttcat aggagatgaa gccatcgata aacctacata tgctacaaag tggccgatac 301 gacatggaat cattgaagac tgggatctta tggaaaggtt catggagcaa gtggttttta 361 aatatcttcg agctgaacct gaggaccatt attttttaat gacagaacct ccactcaata 421 caccagaaaa cagagagtat cttgcagaaa ttatgtttga atcatttaac gtaccaggac 481 tctacattgc agttcaggca gtgctggcct tggcggcatc ttggacatct cgacaagtgg 541 gtgaacgtac gttaacgggg atagtcattg acagcggaga tggagtcacc catgttatcc 601 cagtggcaga aggttatgta attggaagct gcatcaaaca catcccgatt gcaggtagag 661 atattacgta tttcattcaa cagctgctaa gggagaggga ggtgggaatc cctcctgagc 721 agtcactgga gaccgcaaaa gccattaagg agaaatactg ttacattTGC CCCGATATAG 781 TCAAGGAATT TGCCAAGTAT GATGTGGATC CCCGGAAGTG GATCAAACAG TACACGGGTA 841 TCAATGCGAT CAACCAGAAG AAGTTTGTTA TAGACGTTGG TTACGAAAGA TTCCTGGGAC 901 CTGAAATATT CTTTCACCCG GAGTTTGCCA ACCCAGACTT TATGGAGTCC ATCTCAGATG 961 TTGTTGATGA AGTAATACAG AACTGCCCCA TCGATGTGCG GCGCCCGCTG TATAAGCCCG 1021 AGTTCTTTCA GGTCTGCCAC ACCAAGAAGG ACTATGAAGA GTACGGGCCC AGCATCTGCC 1081 GCCACAACCC CGTCTTTGGA GTCATGTCCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAAGTA 1261 AGAACTACCA GACTCACCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt