Transcript: Human XM_011524934.2

PREDICTED: Homo sapiens mbt domain containing 1 (MBTD1), transcript variant X16, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MBTD1 (54799)
Length:
1832
CDS:
216..1583

Additional Resources:

NCBI RefSeq record:
XM_011524934.2
NBCI Gene record:
MBTD1 (54799)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011524934.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000151070 GCCACAGTAACTCGAATTATT pLKO.1 1676 3UTR 100% 15.000 21.000 N MBTD1 n/a
2 TRCN0000323079 GCCACAGTAACTCGAATTATT pLKO_005 1676 3UTR 100% 15.000 21.000 N MBTD1 n/a
3 TRCN0000323080 GCCCATTAATACATCATATTG pLKO_005 1294 CDS 100% 13.200 18.480 N MBTD1 n/a
4 TRCN0000150313 CTCCTAGAACTATTCAGCATA pLKO.1 1021 CDS 100% 4.950 3.960 N MBTD1 n/a
5 TRCN0000154234 CCTCCTAGAACTATTCAGCAT pLKO.1 1020 CDS 100% 2.640 2.112 N MBTD1 n/a
6 TRCN0000323082 GGAAGCTATAGACCCATTAAA pLKO_005 1460 CDS 100% 15.000 10.500 N MBTD1 n/a
7 TRCN0000323011 TCTCATGGAGCCACGTTTAAT pLKO_005 1648 3UTR 100% 15.000 10.500 N MBTD1 n/a
8 TRCN0000097657 CGTGTAGGAATGAAATTAGAA pLKO.1 1619 3UTR 100% 5.625 3.938 N Mbtd1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011524934.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12084 pDONR223 100% 44.4% 41.9% None 1_567del;1297_1298ins148;1365_1366ins284 n/a
2 ccsbBroad304_12084 pLX_304 0% 44.4% 41.9% V5 1_567del;1297_1298ins148;1365_1366ins284 n/a
3 TRCN0000479991 GTTTCTTTAGCCCCGACTGTGCAG pLX_317 25.9% 44.4% 41.9% V5 1_567del;1297_1298ins148;1365_1366ins284 n/a
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