Transcript: Human XM_011534331.2

PREDICTED: Homo sapiens oxidative stress responsive kinase 1 (OXSR1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
OXSR1 (9943)
Length:
4152
CDS:
236..1978

Additional Resources:

NCBI RefSeq record:
XM_011534331.2
NBCI Gene record:
OXSR1 (9943)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148375 AATTGAATTGGCTACAGGGG pXPR_003 CGG 826 47% 7 0.0185 OXSR1 OXSR1 77750
2 BRDN0001147167 CCAACAAGGGGTGCCAACAA pXPR_003 AGG 718 41% 6 -0.1608 OXSR1 OXSR1 77751
3 BRDN0001149240 TGAGAAATGTCAAACTAGCA pXPR_003 TGG 325 19% 2 -0.2846 OXSR1 OXSR1 77749
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011534331.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196623 GAGCACTCATAATGCAATATG pLKO.1 3119 3UTR 100% 13.200 18.480 N OXSR1 n/a
2 TRCN0000194994 CGGAAGGGATTTAGTAATAGT pLKO.1 1819 CDS 100% 5.625 4.500 N OXSR1 n/a
3 TRCN0000001586 GCGTATCTCTGTTGCTTCTAT pLKO.1 2021 3UTR 100% 5.625 4.500 N OXSR1 n/a
4 TRCN0000234391 AGGTTCTGTTCTGGATATTAT pLKO_005 685 CDS 100% 15.000 10.500 N OXSR1 n/a
5 TRCN0000234392 TAGCAACTGGTGGTGATATTA pLKO_005 906 CDS 100% 15.000 10.500 N OXSR1 n/a
6 TRCN0000234393 GTAATAGTGGCAGCTAATTTG pLKO_005 1832 CDS 100% 13.200 9.240 N OXSR1 n/a
7 TRCN0000234390 GTGGCAATCAAACGGATAAAC pLKO_005 521 CDS 100% 13.200 9.240 N OXSR1 n/a
8 TRCN0000001588 GCCATCATCCTAATATTGTAT pLKO.1 603 CDS 100% 5.625 3.938 N OXSR1 n/a
9 TRCN0000001587 GCAGAACTATTAAGGCACAAA pLKO.1 1241 CDS 100% 4.950 3.465 N OXSR1 n/a
10 TRCN0000010643 GTGGAGGTTCTGTTCTGGATA pLKO.1 681 CDS 100% 4.950 3.465 N OXSR1 n/a
11 TRCN0000001589 CCTGATGATGGTAAACTGATA pLKO.1 1931 CDS 100% 4.950 2.970 N OXSR1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011534331.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14949 pDONR223 0% 89.1% 85.9% None (many diffs) n/a
2 ccsbBroad304_14949 pLX_304 0% 89.1% 85.9% V5 (many diffs) n/a
3 TRCN0000468244 TGCTCTTTCACGCTGTCTCTGAAC pLX_317 25.8% 89.1% 85.9% V5 (many diffs) n/a
4 TRCN0000489498 AGAGCAACCAAATGCAAACTATGA pLX_317 25.9% 89.1% 85.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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