Transcript: Human XM_011534537.2

PREDICTED: Homo sapiens G protein-coupled receptor kinase 6 (GRK6), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GRK6 (2870)
Length:
2480
CDS:
30..1682

Additional Resources:

NCBI RefSeq record:
XM_011534537.2
NBCI Gene record:
GRK6 (2870)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011534537.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199094 CCGCTCACTTTGCTCACAGCT pLKO.1 1160 CDS 100% 0.880 1.232 N GRK6 n/a
2 TRCN0000199727 GCGGCAGCTAACGCAGAATTT pLKO.1 218 CDS 100% 13.200 9.240 N GRK6 n/a
3 TRCN0000195556 CGAATGTATATAGCGACCAGA pLKO.1 1905 3UTR 100% 2.640 1.848 N GRK6 n/a
4 TRCN0000001367 CCTCGACAGCATCTACTTCAA pLKO.1 395 CDS 100% 4.950 2.970 N GRK6 n/a
5 TRCN0000001369 CAGTAGGTTTGTAGTGAGCTT pLKO.1 638 CDS 100% 2.640 1.584 N GRK6 n/a
6 TRCN0000001368 CAGCATCTACTTCAACCGTTT pLKO.1 401 CDS 100% 4.050 2.025 Y GRK6 n/a
7 TRCN0000010619 CTGAATGTCTTTGGGCTGGAT pLKO.1 1482 CDS 100% 2.640 1.320 Y GRK6 n/a
8 TRCN0000199994 CGAGGAGTATTCCGAGCGCTT pLKO.1 1127 CDS 100% 0.720 0.360 Y GRK6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011534537.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06322 pDONR223 100% 91.5% 82.2% None (many diffs) n/a
2 ccsbBroad304_06322 pLX_304 0% 91.5% 82.2% V5 (many diffs) n/a
3 TRCN0000479803 TGAGACGCGACATGGTATATCCAG pLX_317 20.5% 91.5% 82.2% V5 (many diffs) n/a
4 ccsbBroadEn_14658 pDONR223 0% 91.5% 82.2% None (many diffs) n/a
5 ccsbBroad304_14658 pLX_304 0% 91.5% 82.2% V5 (many diffs) n/a
6 ccsbBroadEn_00682 pDONR223 100% 89.5% 78.1% None (many diffs) n/a
7 ccsbBroad304_00682 pLX_304 0% 89.5% 78.1% V5 (many diffs) n/a
8 TRCN0000480454 ACTTTTCCCGCTTGTCCTGCCCCC pLX_317 24.5% 89.5% 78.1% V5 (many diffs) n/a
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