Transcript: Human XM_011534538.1

PREDICTED: Homo sapiens G protein-coupled receptor kinase 6 (GRK6), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GRK6 (2870)
Length:
1922
CDS:
150..1124

Additional Resources:

NCBI RefSeq record:
XM_011534538.1
NBCI Gene record:
GRK6 (2870)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146643 GTGCTGACACTGATGAACGG pXPR_003 GGG 5 1% 2 0.8142 GRK6 GRK6 75704
2 BRDN0001146204 CTGCGATCATCTCGTACAGG pXPR_003 AGG 329 34% 5 -0.0844 GRK6 GRK6 75703
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011534538.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199094 CCGCTCACTTTGCTCACAGCT pLKO.1 602 CDS 100% 0.880 1.232 N GRK6 n/a
2 TRCN0000195556 CGAATGTATATAGCGACCAGA pLKO.1 1347 3UTR 100% 2.640 1.848 N GRK6 n/a
3 TRCN0000010619 CTGAATGTCTTTGGGCTGGAT pLKO.1 924 CDS 100% 2.640 1.320 Y GRK6 n/a
4 TRCN0000199994 CGAGGAGTATTCCGAGCGCTT pLKO.1 569 CDS 100% 0.720 0.360 Y GRK6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011534538.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06322 pDONR223 100% 54.8% 54.8% None (many diffs) n/a
2 ccsbBroad304_06322 pLX_304 0% 54.8% 54.8% V5 (many diffs) n/a
3 TRCN0000479803 TGAGACGCGACATGGTATATCCAG pLX_317 20.5% 54.8% 54.8% V5 (many diffs) n/a
4 ccsbBroadEn_14658 pDONR223 0% 54.8% 54.8% None (many diffs) n/a
5 ccsbBroad304_14658 pLX_304 0% 54.8% 54.8% V5 (many diffs) n/a
6 ccsbBroadEn_00682 pDONR223 100% 52.8% 50.5% None 0_1ins795;883_884delAG;936_972del n/a
7 ccsbBroad304_00682 pLX_304 0% 52.8% 50.5% V5 0_1ins795;883_884delAG;936_972del n/a
8 TRCN0000480454 ACTTTTCCCGCTTGTCCTGCCCCC pLX_317 24.5% 52.8% 50.5% V5 0_1ins795;883_884delAG;936_972del n/a
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