Transcript: Human XM_011537431.2

PREDICTED: Homo sapiens tetratricopeptide repeat domain 6 (TTC6), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TTC6 (319089)
Length:
4219
CDS:
84..4055

Additional Resources:

NCBI RefSeq record:
XM_011537431.2
NBCI Gene record:
TTC6 (319089)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011537431.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000148684 CCAAAGAACTACCTAGCCTAT pLKO.1 3426 CDS 100% 4.050 3.240 N TTC6 n/a
2 TRCN0000149730 GCAGGATATTAATGCTGCCAT pLKO.1 3497 CDS 100% 2.640 2.112 N TTC6 n/a
3 TRCN0000149712 GATGACCATGTGTGCTCTATT pLKO.1 2345 CDS 100% 13.200 9.240 N TTC6 n/a
4 TRCN0000147652 GTTCTCATGAATCGAGCTATT pLKO.1 3744 CDS 100% 10.800 7.560 N TTC6 n/a
5 TRCN0000147030 CCACAAACAGAATGCAATGAA pLKO.1 3578 CDS 100% 5.625 3.938 N TTC6 n/a
6 TRCN0000149767 GCTTGGAACCACTTTACCATT pLKO.1 3390 CDS 100% 4.950 3.465 N TTC6 n/a
7 TRCN0000146725 CAATATGAACTAGCTGAGGAA pLKO.1 3885 CDS 100% 2.640 1.848 N TTC6 n/a
8 TRCN0000147365 GATTATGGAATTGTGCTGCTT pLKO.1 2973 CDS 100% 2.640 1.848 N TTC6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011537431.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13052 pDONR223 100% 45.3% 45.3% None 1_2145del;2601_2624del n/a
2 ccsbBroad304_13052 pLX_304 0% 45.3% 45.3% V5 1_2145del;2601_2624del n/a
3 TRCN0000479568 TTAGACATAACAGTTCAAAAAAGC pLX_317 20.6% 45.3% 45.3% V5 1_2145del;2601_2624del n/a
4 ccsbBroadEn_13053 pDONR223 100% 37.8% 36.3% None (many diffs) n/a
5 ccsbBroad304_13053 pLX_304 0% 37.8% 36.3% V5 (many diffs) n/a
6 TRCN0000477660 GATAGCAACATACCAAAAAGTTCT pLX_317 23.4% 37.8% 36.3% V5 (many diffs) n/a
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