Transcript: Human XM_011541013.3

PREDICTED: Homo sapiens FGR proto-oncogene, Src family tyrosine kinase (FGR), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FGR (2268)
Length:
1355
CDS:
197..1255

Additional Resources:

NCBI RefSeq record:
XM_011541013.3
NBCI Gene record:
FGR (2268)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011541013.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199966 CCCTGTTCATTGCCCTGTATG pLKO.1 435 CDS 100% 10.800 15.120 N FGR n/a
2 TRCN0000001597 ACCCTGTTCATTGCCCTGTAT pLKO.1 434 CDS 100% 4.950 3.465 N FGR n/a
3 TRCN0000001596 CACATCCTGAACAATACTGAA pLKO.1 509 CDS 100% 4.950 3.465 N FGR n/a
4 TRCN0000001594 CTTCCTTGATAGTGGCACCAT pLKO.1 388 CDS 100% 2.640 1.848 N FGR n/a
5 TRCN0000199653 GCCTTTCTCATTCGGGAAAGC pLKO.1 695 CDS 100% 0.405 0.284 N FGR n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011541013.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00564 pDONR223 100% 66.5% 64.6% None 1017_1018insGGCA;1056_1057ins527 n/a
2 ccsbBroad304_00564 pLX_304 0% 66.5% 64.6% V5 1017_1018insGGCA;1056_1057ins527 n/a
3 TRCN0000478102 CGGACTTCGCGTGCGTAGGTCTTC pLX_317 21.4% 66.5% 64.6% V5 1017_1018insGGCA;1056_1057ins527 n/a
4 ccsbBroadEn_14641 pDONR223 0% 66.5% 64.6% None 1017_1018insGGCA;1056_1057ins527 n/a
5 ccsbBroad304_14641 pLX_304 0% 66.5% 64.6% V5 1017_1018insGGCA;1056_1057ins527 n/a
6 TRCN0000472499 CTCTCTAATATTTACACGCAAGGT pLX_317 26.9% 66.5% 64.6% V5 1017_1018insGGCA;1056_1057ins527 n/a
7 TRCN0000492193 ACATCTCGAATCGGTTGGCTTTCC pLX_317 11% 66.5% 64.6% V5 (not translated due to prior stop codon) 1017_1018insGGCA;1056_1057ins527 n/a
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