Construct: ORF TRCN0000491277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021834.2_s317c1
- DNA Barcode:
- ATCCTAACGTAATTTTTTAATGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- IL12RB2 (3595)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491277
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001374259.1 | 99.9% | 100% | 714T>C |
| 2 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001559.2 | 99.9% | 100% | 714T>C |
| 3 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_005270827.2 | 99.9% | 100% | 714T>C |
| 4 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_005270828.3 | 99.9% | 100% | 714T>C |
| 5 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_011541383.2 | 99.9% | 100% | 714T>C |
| 6 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_011541384.2 | 93.8% | 93.8% | 714T>C;1557_1558ins159 |
| 7 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001258215.1 | 89.9% | 90% | 714T>C;1458_1459ins258 |
| 8 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_006710617.2 | 89.9% | 90% | 714T>C;1458_1459ins258 |
| 9 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001258214.1 | 76.4% | 75.2% | 714T>C;1946_1947ins100;1977_1978ins509 |
| 10 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001319233.1 | 76.4% | 75.2% | 714T>C;1946_1947ins100;1977_1978ins509 |
| 11 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_017001203.1 | 76.4% | 75.2% | 714T>C;1946_1947ins100;1977_1978ins509 |
| 12 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001258216.1 | 73.6% | 72% | 714T>C;1853_1854ins191;1905_1906ins490 |
| 13 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NR_047584.1 | 62.5% | (many diffs) | |
| 14 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NR_047583.1 | 57.7% | (many diffs) | |
| 15 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_017001204.1 | 57.1% | 56.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2658
- ORF length:
- 2586
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcacat acttttagag gatgctcatt ggcatttatg tttataatca 121 cgtggctgtt gattaaagca aaaatagatg cgtgcaagag aggcgatgtg actgtgaagc 181 cttcccatgt aattttactt ggatccactg tcaatattac atgctctttg aagcccagac 241 aaggctgctt tcactattcc agacgtaaca agttaatcct gtacaagttt gacagaagaa 301 tcaattttca ccatggccac tccctcaatt ctcaagtcac aggtcttccc cttggtacaa 361 ccttgtttgt ctgcaaactg gcctgtatca atagtgatga aattcaaata tgtggagcag 421 agatcttcgt tggtgttgct ccagaacagc ctcaaaattt atcctgcata cagaagggag 481 aacaggggac tgtggcctgc acctgggaaa gaggacgaga cacccactta tacactgagt 541 atactctaca gctaagtgga ccaaaaaatt taacctggca gaagcaatgt aaagacattt 601 attgtgacta tttggacttt ggaatcaacc tcacccctga atcacctgaa tccaatttca 661 cagccaaggt tactgctgtc aatagtcttg gaagctcctc ttcacttcca tccacattca 721 cattcttgga catagtgagg cctcttcctc cgtgggacat tagaatcaaa tttcaaaagg 781 cttccgtgag cagatgtacc ctttattgga gagatgaggg actggtactg cttaatcgac 841 tcagatatcg gcccagtaac agcaggctct ggaatatggt taatgttaca aaggccaaag 901 gaagacatga tttgctggat ctgaaaccat ttacagaata tgaatttcag atttcctcta 961 agctacatct ttataaggga agttggagtg attggagtga atcattgaga gcacaaacac 1021 cagaagaaga gcctactggg atgttagatg tctggtacat gaaacggcac attgactaca 1081 gtagacaaca gatttctctt ttctggaaga atctgagtgt ctcagaggca agaggaaaaa 1141 ttctccacta tcaggtgacc ttgcaggagc tgacaggagg gaaagccatg acacagaaca 1201 tcacaggaca cacctcctgg accacagtca ttcctagaac cggaaattgg gctgtggctg 1261 tgtctgcagc aaattcaaaa ggcagttctc tgcccactcg tattaacata atgaacctgt 1321 gtgaggcagg gttgctggct cctcgccagg tctctgcaaa ctcagagggc atggacaaca 1381 ttctggtgac ttggcagcct cccaggaaag atccctctgc tgttcaggag tacgtggtgg 1441 aatggagaga gctccatcca gggggtgaca cacaggtccc tctaaactgg ctacggagtc 1501 gaccctacaa tgtgtctgct ctgatttcag agaacataaa atcctacatc tgttatgaaa 1561 tccgtgtgta tgcactctca ggggatcaag gaggatgcag ctccatcctg ggtaactcta 1621 agcacaaagc accactgagt ggcccccaca ttaatgccat cacagaggaa aaggggagca 1681 ttttaatttc atggaacagc attccagtcc aggagcaaat gggctgcctc ctccattata 1741 ggatatactg gaaggaacgg gactccaact cccagcctca gctctgtgaa attccctaca 1801 gagtctccca aaattcacat ccaataaaca gcctgcagcc ccgagtgaca tatgtcctgt 1861 ggatgacagc tctgacagct gctggtgaaa gttcccacgg aaatgagagg gaattttgtc 1921 tgcaaggtaa agccaattgg atggcgtttg tggcaccaag catttgcatt gctatcatca 1981 tggtgggcat tttctcaacg cattacttcc agcaaaaggt gtttgttctc ctagcagccc 2041 tcagacctca gtggtgtagc agagaaattc cagatccagc aaatagcact tgcgctaaga 2101 aatatcccat tgcagaggag aagacacagc tgcccttgga caggctcctg atagactggc 2161 ccacgcctga agatcctgaa ccgctggtca tcagtgaagt ccttcatcaa gtgaccccag 2221 ttttcagaca tcccccctgc tccaactggc cacaaaggga aaaaggaatc caaggtcatc 2281 aggcctctga gaaagacatg atgcacagtg cctcaagccc accacctcca agagctctcc 2341 aagctgagag cagacaactg gtggatctgt acaaggtgct ggagagcagg ggcTCCGACC 2401 CAAAGCCCGA AAACCCAGCC TGTCCCTGGA CGGTGCTCCC AGCAGGTGAC CTTCCCACCC 2461 ATGATGGCTA CTTACCCTCC AACATAGATG ACCTCCCCTC ACATGAGGCA CCTCTCGCTG 2521 ACTCTCTGGA AGAACTGGAG CCTCAGCACA TCTCCCTTTC TGTTTTCCCC TCAAGTTCTC 2581 TTCACCCACT CACCTTCTCC TGTGGTGATA AGCTGACTCT GGATCAGTTA AAGATGAGGT 2641 GTGACTCCCT CATGCTCTAG AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2701 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2761 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATCC TAACGTAATT 2821 TTTTAATGAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt