Transcript: Human XM_011544988.2

PREDICTED: Homo sapiens ankyrin repeat domain 13D (ANKRD13D), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ANKRD13D (338692)
Length:
1560
CDS:
356..1408

Additional Resources:

NCBI RefSeq record:
XM_011544988.2
NBCI Gene record:
ANKRD13D (338692)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011544988.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000162319 CTGAGAAGATGGAAACTGTTA pLKO.1 194 5UTR 100% 4.950 3.465 N ANKRD13D n/a
2 TRCN0000165930 GACACTCGTAATGTGGCCTTT pLKO.1 139 5UTR 100% 4.050 2.835 N ANKRD13D n/a
3 TRCN0000165095 GAAGACTCCATTCCAGTCCTT pLKO.1 328 5UTR 100% 2.640 1.848 N ANKRD13D n/a
4 TRCN0000165836 GATGGAAACTGTTAGCGGCTA pLKO.1 201 5UTR 100% 2.160 1.512 N ANKRD13D n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011544988.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16157 pDONR223 0% 71.6% 71% None (many diffs) n/a
2 ccsbBroad304_16157 pLX_304 0% 71.6% 71% V5 (many diffs) n/a
3 TRCN0000465531 TGACTCCTCTATTCTTTGGCACAT pLX_317 47.1% 71.6% 71% V5 (many diffs) n/a
4 ccsbBroadEn_16158 pDONR223 0% 53% 53% None 0_1ins651;160_306del;573T>C n/a
5 ccsbBroad304_16158 pLX_304 0% 53% 53% V5 0_1ins651;160_306del;573T>C n/a
6 ccsbBroadEn_14474 pDONR223 100% 52.9% 52.9% None (many diffs) n/a
7 ccsbBroad304_14474 pLX_304 0% 52.9% 52.9% V5 (many diffs) n/a
8 TRCN0000476082 TCCGCTAAGCCGGGTGGCATAATT pLX_317 23.2% 52.9% 52.9% V5 (many diffs) n/a
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