Transcript: Human XM_017002771.1

PREDICTED: Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAP3K6 (9064)
Length:
3729
CDS:
908..3517

Additional Resources:

NCBI RefSeq record:
XM_017002771.1
NBCI Gene record:
MAP3K6 (9064)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017002771.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000002346 AGCGTATTAAACAGAACACTT pLKO.1 3699 3UTR 100% 4.950 6.930 N MAP3K6 n/a
2 TRCN0000002345 CGTGGAGAAGATGCAGTATTA pLKO.1 943 CDS 100% 13.200 9.240 N MAP3K6 n/a
3 TRCN0000352611 CGTGGAGAAGATGCAGTATTA pLKO_005 943 CDS 100% 13.200 9.240 N MAP3K6 n/a
4 TRCN0000196920 GAGCTGAATGAGGGCATCATA pLKO.1 3518 CDS 100% 5.625 3.938 N MAP3K6 n/a
5 TRCN0000002343 GTTGGAGTTTGATTATGAGTA pLKO.1 1564 CDS 100% 4.950 3.465 N MAP3K6 n/a
6 TRCN0000352677 GTTGGAGTTTGATTATGAGTA pLKO_005 1564 CDS 100% 4.950 3.465 N MAP3K6 n/a
7 TRCN0000199055 CAAGCCTTTCTCCTCCGAACT pLKO.1 2288 CDS 100% 4.050 2.835 N MAP3K6 n/a
8 TRCN0000352612 CAAGCCTTTCTCCTCCGAACT pLKO_005 2288 CDS 100% 4.050 2.835 N MAP3K6 n/a
9 TRCN0000002344 CATGTTCTTCAGCTCGGGTTT pLKO.1 724 5UTR 100% 4.050 2.835 N MAP3K6 n/a
10 TRCN0000197244 GATGAATGGAGAGGACAAAGG pLKO.1 3560 3UTR 100% 4.050 2.835 N MAP3K6 n/a
11 TRCN0000342400 GATGAATGGAGAGGACAAAGG pLKO_005 3560 3UTR 100% 4.050 2.835 N MAP3K6 n/a
12 TRCN0000002342 CAACCATTCAAGACAGCCTGT pLKO.1 1187 CDS 100% 2.160 1.512 N MAP3K6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017002771.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11332 pDONR223 100% 64.1% 63.7% None (many diffs) n/a
2 ccsbBroad304_11332 pLX_304 0% 64.1% 63.7% V5 (many diffs) n/a
3 TRCN0000481050 TGATCAGTTTTTGCCATGAGCGTT pLX_317 11.9% 64.1% 63.7% V5 (many diffs) n/a
4 ccsbBroadEn_14926 pDONR223 0% 64.1% 63.7% None (many diffs) n/a
5 ccsbBroad304_14926 pLX_304 0% 64.1% 63.7% V5 (many diffs) n/a
6 TRCN0000465376 CGCAATTACCGCCGTCAACACTGT pLX_317 11.6% 64.1% 63.7% V5 (many diffs) n/a
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