Construct: ORF TRCN0000465376
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006296.2_s317c1
- Derived from:
- ccsbBroadEn_14926
- DNA Barcode:
- CGCAATTACCGCCGTCAACACTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K6 (9064)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465376
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9064 | MAP3K6 | mitogen-activated protein k... | XM_017002771.1 | 64.1% | 63.7% | (many diffs) |
| 2 | human | 9064 | MAP3K6 | mitogen-activated protein k... | NM_001297609.2 | 61.8% | 61.4% | (many diffs) |
| 3 | human | 9064 | MAP3K6 | mitogen-activated protein k... | NM_004672.5 | 61.4% | 61.1% | (many diffs) |
| 4 | human | 9064 | MAP3K6 | mitogen-activated protein k... | XR_946794.3 | 48.4% | (many diffs) | |
| 5 | human | 9064 | MAP3K6 | mitogen-activated protein k... | XM_017002772.1 | 47.1% | 46.9% | 1_831del;2320_2356del;2403_2404ins856 |
| 6 | human | 9064 | MAP3K6 | mitogen-activated protein k... | XR_946795.3 | 42.9% | (many diffs) | |
| 7 | mouse | 53608 | Map3k6 | mitogen-activated protein k... | XM_006539040.3 | 52.7% | 55.3% | (many diffs) |
| 8 | mouse | 53608 | Map3k6 | mitogen-activated protein k... | NM_016693.5 | 52.4% | 55% | (many diffs) |
| 9 | mouse | 53608 | Map3k6 | mitogen-activated protein k... | XM_006539041.3 | 29.3% | 30.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2457
- ORF length:
- 2391
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cttgctgctc tcctaccgcg atgtgcagga ctactcggcc atcattgagc 121 tggtggagac gctgcaggcc ttgcccacct gtgatgtggc cgagcagcat aatgtctgct 181 tccactacac ttttgccctc aaccggagga acaggcctgg ggaccgggcg aaggccctgt 241 ctgtgctgct gccgctggta cagcttgagg gctctgtggc gcccgatctg tactgcatgt 301 gtggccgtat ctacaaggac atgttcttca gctcgggttt ccaggatgct gggcaccggg 361 agcaggccta tcactggtat cgcaaggctt ttgacgtaga gcccagcctt cactcaggca 421 tcaatgcagc tgtgctcctc attgctgccg ggcagcactt tgaggattcc aaagagctcc 481 ggctaatagg catgaagctg ggctgcctgc tggcccgcaa aggctgcgtg gagaagatgc 541 agtattactg ggatgtgggt ttctacctgg gagcccagat cctcgccaat gaccccaccc 601 aggtggtgct ggctgcagag cagctgtata agctcaatgc ccccatatgg tacctggtgt 661 ccgtgatgga gaccttcctg ctctaccagc acttcaggcc cacgccagag ccccctggag 721 ggccaccacg ccgtgcccac ttctggctcc acttcttgct acagtcctgc caaccattca 781 agacagcctg tgcccagggc gaccagtgct tggtgctggt cctggagatg aacaaggtgc 841 tgctgcctgc aaagctcgag gttcggggta ctgacccagt aagcacagtg accctgagcc 901 tgctggagcc tgagacccag gacattccct ccagctggac cttcccagtc gcctccatat 961 gcggagtcag cgcctcaaag cgcgacgagc gctgctgctt cctctatgca ctccccccgg 1021 ctcaggacgt ccagctgtgc ttccccagcg tagggcactg ccagtggttc tgcggcctga 1081 tccaggcctg ggtgacgaac ccggattcca cggcgcccgc ggaggaggcg gagggcgcgg 1141 gggagatgtt ggagtttgat tatgagtaca cggagacggg cgagcggctg gtgctgggca 1201 agggcacgta tggggtggtg tacgcgggcc gcgatcgcca cacgagggtg cgcatcgcca 1261 tcaaggagat cccggagcgg gacagcaggt tctctcagcc cctgcatgaa gagatcgctc 1321 ttcacagacg cctgcgccac aagaacatag tgcgctatct gggctcagct agccagggcg 1381 gctaccttaa gatcttcatg gaggaagtgc ctggaggcag cctgtcctcc ttgctgcggt 1441 cggtgtgggg acccctgaag gacaacgaga gcaccatcag tttctacacc cgccagatcc 1501 tgcagggact tggctacttg cacgacaacc acatcgtgca cagggacata aaaggggaca 1561 atgtgctgat caacaccttc agtgggctgc tcaagatttc tgacttcggc acctccaagc 1621 ggctggcagg catcacacct tgcactgaga ccttcacagg aactctgcag tatatggccc 1681 cagaaatcat tgaccagggc ccacgcgggt atgggaaagc agctgacatc tggtcactgg 1741 gctgcactgt cattgagatg gccacaggtc gccccccctt ccacgagctc gggagcccac 1801 aggctgccat gtttcaggtg ggtatgtaca aggtccatcc gccaatgccc agctctctgt 1861 cggccgaggc ccaagccttt ctcctccgaa cttttgagcc agacccccgc ctccgagcca 1921 gcgcccagac actgctgggg gaccccttcc tgcagcctgg gaaaaggagc cgcagcccca 1981 gctccccacg acatgctcca cggccctcag atgccccttc tgccagtccc actccttcag 2041 ccaactcaac cacccagtct cagacattcc cgtgccctca ggcaccctct cagcacccac 2101 ccagcccccc gaagcgctgc ctcagttatg ggggcaccaa ccagctccgg gtgcccgagg 2161 agcctgcggc cgaggagcct gcgtcTCCGG AGGAGAGTTC GGGGCTGAGC CTGCTGCACC 2221 AGGAGAGCAA GCGTCGGGCC ATGCTGGCCG CAGTATTGGA GCAGGAGCTG CCAGCGCTGG 2281 CGGAGAATCT GCACCAGGAG CAGAAGCAAG AGCAGGGGGC CCGTCTGGGC AGAAACCATG 2341 TGGAAGAGCT GCTGCGCTGC CTCGGGGCAC ACATCCACAC TCCCAACCGC CGGCAGCTCG 2401 CCCAGGAGCT GCGGGCGCTG CAAGGATGGA TGAATGGAGA GGACAAAGGC AGCTTCTGCC 2461 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2521 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2581 ATATATCTTG TGGAAAGGAC GACGCAATTA CCGCCGTCAA CACTGTACGC GTTAAGTCga 2641 caatcaacct ctggattaca aaatttgtga aagatt