Construct: ORF TRCN0000481050

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006296.3_s317c1
Derived from:
ccsbBroadEn_11332
DNA Barcode:
TGATCAGTTTTTGCCATGAGCGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAP3K6 (9064)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481050
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9064 MAP3K6 mitogen-activated protein k... XM_017002771.1 64.1% 63.7% (many diffs)
2 human 9064 MAP3K6 mitogen-activated protein k... NM_001297609.2 61.8% 61.4% (many diffs)
3 human 9064 MAP3K6 mitogen-activated protein k... NM_004672.5 61.4% 61.1% (many diffs)
4 human 9064 MAP3K6 mitogen-activated protein k... XR_946794.3 48.4% (many diffs)
5 human 9064 MAP3K6 mitogen-activated protein k... XM_017002772.1 47.1% 46.9% 1_831del;2320_2356del;2403_2404ins856
6 human 9064 MAP3K6 mitogen-activated protein k... XR_946795.3 42.9% (many diffs)
7 mouse 53608 Map3k6 mitogen-activated protein k... XM_006539040.3 52.7% 55.3% (many diffs)
8 mouse 53608 Map3k6 mitogen-activated protein k... NM_016693.5 52.4% 55% (many diffs)
9 mouse 53608 Map3k6 mitogen-activated protein k... XM_006539041.3 29.3% 30.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2457
ORF length:
2391
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa cttgctgctc tcctaccgcg atgtgcagga ctactcggcc atcattgagc
121tggtggagac gctgcaggcc ttgcccacct gtgatgtggc cgagcagcat aatgtctgct
181tccactacac ttttgccctc aaccggagga acaggcctgg ggaccgggcg aaggccctgt
241ctgtgctgct gccgctggta cagcttgagg gctctgtggc gcccgatctg tactgcatgt
301gtggccgtat ctacaaggac atgttcttca gctcgggttt ccaggatgct gggcaccggg
361agcaggccta tcactggtat cgcaaggctt ttgacgtaga gcccagcctt cactcaggca
421tcaatgcagc tgtgctcctc attgctgccg ggcagcactt tgaggattcc aaagagctcc
481ggctaatagg catgaagctg ggctgcctgc tggcccgcaa aggctgcgtg gagaagatgc
541agtattactg ggatgtgggt ttctacctgg gagcccagat cctcgccaat gaccccaccc
601aggtggtgct ggctgcagag cagctgtata agctcaatgc ccccatatgg tacctggtgt
661ccgtgatgga gaccttcctg ctctaccagc acttcaggcc cacgccagag ccccctggag
721ggccaccacg ccgtgcccac ttctggctcc acttcttgct acagtcctgc caaccattca
781agacagcctg tgcccagggc gaccagtgct tggtgctggt cctggagatg aacaaggtgc
841tgctgcctgc aaagctcgag gttcggggta ctgacccagt aagcacagtg accctgagcc
901tgctggagcc tgagacccag gacattccct ccagctggac cttcccagtc gcctccatat
961gcggagtcag cgcctcaaag cgcgacgagc gctgctgctt cctctatgca ctccccccgg
1021ctcaggacgt ccagctgtgc ttccccagcg tagggcactg ccagtggttc tgcggcctga
1081tccaggcctg ggtgacgaac ccggattcca cggcgcccgc ggaggaggcg gagggcgcgg
1141gggagatgtt ggagtttgat tatgagtaca cggagacggg cgagcggctg gtgctgggca
1201agggcacgta tggggtggtg tacgcgggcc gcgatcgcca cacgagggtg cgcatcgcca
1261tcaaggagat cccggagcgg gacagcaggt tctctcagcc cctgcatgaa gagatcgctc
1321ttcacagacg cctgcgccac aagaacatag tgcgctatct gggctcagct agccagggcg
1381gctaccttaa gatcttcatg gaggaagtgc ctggaggcag cctgtcctcc ttgctgcggt
1441cggtgtgggg acccctgaag gacaacgaga gcaccatcag tttctacacc cgccagatcc
1501tgcagggact tggctacttg cacgacaacc acatcgtgca cagggacata aaaggggaca
1561atgtgctgat caacaccttc agtgggctgc tcaagatttc tgacttcggc acctccaagc
1621ggctggcagg catcacacct tgcactgaga ccttcacagg aactctgcag tatatggccc
1681cagaaatcat tgaccagggc ccacgcgggt atgggaaagc agctgacatc tggtcactgg
1741gctgcactgt cattgagatg gccacaggtc gccccccctt ccacgagctc gggagcccac
1801aggctgccat gtttcaggtg ggtatgtaca aggtccatcc gccaatgccc agctctctgt
1861cggccgaggc ccaagccttt ctcctccgaa cttttgagcc agacccccgc ctccgagcca
1921gcgcccagac actgctgggg gaccccttcc tgcagcctgg gaaaaggagc cgcagcccca
1981gctccccacg acatgctcca cggccctcag atgccccttc tgccagtccc actccttcag
2041ccaactcaac cacccagtct cagacattcc cgtgccctca ggcaccctct cagcacccac
2101ccagcccccc gaagcgctgc ctcagttatg ggggcaccaa ccagctccgg gtgcccgagg
2161agcctgcggc cgaggagccT GCGTCTCCGG AGGAGAGTTC GGGGCTGAGC CTGCTGCACC
2221AGGAGAGCAA GCGTCGGGCC ATGCTGGCCG CAGTATTGGA GCAGGAGCTG CCAGCGCTGG
2281CGGAGAATCT GCACCAGGAG CAGAAGCAAG AGCAGGGGGC CCGTCTGGGC AGAAACCATG
2341TGGAAGAGCT GCTGCGCTGC CTCGGGGCAC ACATCCACAC TCCCAACCGC CGGCAGCTCG
2401CCCAGGAGCT GCGGGCGCTG CAAGGATGGA TGAATGGAGA GGACAAAGGC AGCTTCTGCC
2461CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
2521TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
2581ATATATCTTG TGGAAAGGAC GATGATCAGT TTTTGCCATG AGCGTTACGC GTTAAGTCga
2641caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)