Transcript: Human XM_017004050.1

PREDICTED: Homo sapiens ankyrin repeat domain 36 (ANKRD36), transcript variant X43, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ANKRD36 (375248)
Length:
4694
CDS:
347..4495

Additional Resources:

NCBI RefSeq record:
XM_017004050.1
NBCI Gene record:
ANKRD36 (375248)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017004050.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000155910 CCACAAGTGATGAGAAGGATT pLKO.1 1533 CDS 100% 4.950 2.970 N ANKRD36 n/a
2 TRCN0000365720 TCGCGTCACTGCACCTATTTA pLKO_005 4235 CDS 100% 15.000 7.500 Y ANKRD36 n/a
3 TRCN0000370949 CTCCTGAGCAACCGCCTTTAT pLKO_005 253 5UTR 100% 13.200 6.600 Y ANKRD36 n/a
4 TRCN0000365723 CACGTGCAAAGTGAGCTAAAG pLKO_005 3797 CDS 100% 10.800 5.400 Y ANKRD36 n/a
5 TRCN0000365719 TCGATGTGATCATGATCAAAG pLKO_005 3580 CDS 100% 10.800 5.400 Y ANKRD36 n/a
6 TRCN0000152758 GCTACAAGTGACGACAAAGAT pLKO.1 410 CDS 100% 5.625 2.813 Y ANKRD36C n/a
7 TRCN0000154273 GCTACAAGTGACGAGAAAGAT pLKO.1 512 CDS 100% 5.625 2.813 Y ANKRD36C n/a
8 TRCN0000365721 ATGAAGATATGGACATTTCTG pLKO_005 4496 CDS 100% 4.950 2.475 Y ANKRD36 n/a
9 TRCN0000167206 CAAGAAATACAGGATCAACTT pLKO.1 4310 CDS 100% 4.950 2.475 Y ANKRD36B n/a
10 TRCN0000153451 CACTGTGAGCAACTTAGAGTA pLKO.1 2912 CDS 100% 4.950 2.475 Y ANKRD36C n/a
11 TRCN0000167527 GAGAAAGCAGAAAGAGAAGTA pLKO.1 4136 CDS 100% 4.950 2.475 Y ANKRD36B n/a
12 TRCN0000154147 CGAGGAAGATTCTGTTTCGAT pLKO.1 931 CDS 100% 3.000 1.500 Y ANKRD36C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017004050.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10271 pDONR223 100% 7.8% 5.9% None (many diffs) n/a
2 ccsbBroad304_10271 pLX_304 0% 7.8% 5.9% V5 (many diffs) n/a
3 TRCN0000478647 CGCTGAAAACACGAATTGAACTCC pLX_317 89.1% 7.8% 5.9% V5 (many diffs) n/a
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