Transcript: Human XM_017008865.2

PREDICTED: Homo sapiens microfibril associated protein 3 like (MFAP3L), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MFAP3L (9848)
Length:
8034
CDS:
1951..3258

Additional Resources:

NCBI RefSeq record:
XM_017008865.2
NBCI Gene record:
MFAP3L (9848)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017008865.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000421526 ATCGTCATGGTCCTCAATATC pLKO_005 2515 CDS 100% 13.200 18.480 N MFAP3L n/a
2 TRCN0000444561 CCTTCTCAGACCGAGGTAAAT pLKO_005 2375 CDS 100% 13.200 10.560 N MFAP3L n/a
3 TRCN0000116946 GTGTGTGGCTTCTAACATCTA pLKO.1 2400 CDS 100% 4.950 3.960 N MFAP3L n/a
4 TRCN0000116943 CCACAGTTCAAGTGGTATAAT pLKO.1 2254 CDS 100% 15.000 10.500 N MFAP3L n/a
5 TRCN0000116944 GCCAGAACTGACCATATCATA pLKO.1 2179 CDS 100% 5.625 3.938 N MFAP3L n/a
6 TRCN0000116942 GCCTTGATTAACTGTAGTGTT pLKO.1 2218 CDS 100% 4.950 3.465 N MFAP3L n/a
7 TRCN0000116945 CAGGACTATCATGGAGGAGAT pLKO.1 2778 CDS 100% 4.050 2.835 N MFAP3L n/a
8 TRCN0000424362 TTACGAAAGCCATGTCTAATA pLKO_005 3240 CDS 100% 13.200 7.920 N MFAP3L n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017008865.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11423 pDONR223 100% 23.2% 22.9% None (many diffs) n/a
2 ccsbBroad304_11423 pLX_304 0% 23.2% 22.9% V5 (many diffs) n/a
3 TRCN0000469608 AGTGCGCAACGAGGCCGGCCCCCA pLX_317 100% 23.2% 22.9% V5 (many diffs) n/a
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