Transcript: Human XM_017009060.1

PREDICTED: Homo sapiens casein kinase 1 gamma 3 (CSNK1G3), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CSNK1G3 (1456)
Length:
4664
CDS:
844..2289

Additional Resources:

NCBI RefSeq record:
XM_017009060.1
NBCI Gene record:
CSNK1G3 (1456)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145821 AGGCTGACACATTAAAGGAG pXPR_003 AGG 849 59% 10 0.5117 CSNK1G3 CSNK1G3 77361
2 BRDN0001145081 ATAGCATTGTATTTACCACA pXPR_003 AGG 399 28% 7 0.1916 CSNK1G3 CSNK1G3 77362
3 BRDN0001144972 AACTTCTTAATAGGACGACC pXPR_003 AGG 590 41% 8 -0.0106 CSNK1G3 CSNK1G3 77363
4 BRDN0001146183 CGATCGGGACACAACACTCG pXPR_003 AGG 77 5% 3 -0.6507 CSNK1G3 CSNK1G3 77360
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017009060.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000355670 TTTCGGCCCTTGTGGTAAATA pLKO_005 1230 CDS 100% 15.000 21.000 N CSNK1G3 n/a
2 TRCN0000338169 TAATGGTTGGACCTAACTTTA pLKO_005 953 CDS 100% 13.200 18.480 N CSNK1G3 n/a
3 TRCN0000196591 GCAACATATCTTCGTTATGTA pLKO.1 1771 CDS 100% 0.563 0.788 N CSNK1G3 n/a
4 TRCN0000338228 CAAAGTAGAAGAGACGATTTA pLKO_005 1594 CDS 100% 13.200 10.560 N CSNK1G3 n/a
5 TRCN0000010550 CCCAGCAAGTTATTCACATTA pLKO.1 1448 CDS 100% 13.200 10.560 N CSNK1G3 n/a
6 TRCN0000196897 GCTAGATATATGAGCATAAAC pLKO.1 1555 CDS 100% 13.200 9.240 N CSNK1G3 n/a
7 TRCN0000196403 GTGATGTATGTTGGAGTTATA pLKO.1 3105 3UTR 100% 13.200 9.240 N CSNK1G3 n/a
8 TRCN0000420971 TAGGATCTGGAGATGGTATAC pLKO_005 1196 CDS 100% 10.800 7.560 N Csnk1g3 n/a
9 TRCN0000000808 CACCGAAACTTACTGCTGAAT pLKO.1 3199 3UTR 100% 4.950 3.465 N CSNK1G3 n/a
10 TRCN0000338227 CACCGAAACTTACTGCTGAAT pLKO_005 3199 3UTR 100% 4.950 3.465 N CSNK1G3 n/a
11 TRCN0000195537 CCCTACTGAAGTAGAAGTGAT pLKO.1 2178 CDS 100% 4.950 3.465 N CSNK1G3 n/a
12 TRCN0000000809 CCGGAGACAAAGAAACACATA pLKO.1 1501 CDS 100% 4.950 3.465 N CSNK1G3 n/a
13 TRCN0000338167 CCGGAGACAAAGAAACACATA pLKO_005 1501 CDS 100% 4.950 3.465 N CSNK1G3 n/a
14 TRCN0000000810 AGGACGTTCAAATGCACCCAT pLKO.1 2151 CDS 100% 2.640 1.848 N CSNK1G3 n/a
15 TRCN0000000811 ACTTCTTAATAGGACGACCAA pLKO.1 1418 CDS 100% 2.640 3.696 N CSNK1G3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017009060.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06057 pDONR223 100% 87.8% 87.7% None (many diffs) n/a
2 ccsbBroad304_06057 pLX_304 0% 87.8% 87.7% V5 (many diffs) n/a
3 TRCN0000477806 CGATTTGCAATTGATGCTTGGCAT pLX_317 26.7% 87.8% 87.7% V5 (many diffs) n/a
4 ccsbBroadEn_14603 pDONR223 100% 87.7% 87.3% None (many diffs) n/a
5 ccsbBroad304_14603 pLX_304 0% 87.7% 87.3% V5 (many diffs) n/a
6 TRCN0000467288 CGAATTGCCCCTTACTCTATAGGA pLX_317 11.6% 87.7% 87.3% V5 (many diffs) n/a
7 TRCN0000489721 TTAGACGGACATTGTATCAGTATC pLX_317 94.5% 23.3% 20.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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