Construct: ORF TRCN0000470046
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006571.1_s317c1
- Derived from:
- ccsbBroadEn_12139
- DNA Barcode:
- AGTGATCTGCATAGGTAATGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHIP (55023)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470046
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55023 | PHIP | pleckstrin homology domain ... | XM_017010989.2 | 55.7% | 55.8% | 1_1677del;2563C>T;2571T>A |
| 2 | human | 55023 | PHIP | pleckstrin homology domain ... | XM_017010990.2 | 55.7% | 55.8% | 1_1677del;2563C>T;2571T>A |
| 3 | human | 55023 | PHIP | pleckstrin homology domain ... | XM_011535918.3 | 42.8% | 42.8% | 1_2826del;3712C>T;3720T>A |
| 4 | human | 55023 | PHIP | pleckstrin homology domain ... | XM_005248729.5 | 38.8% | 38.8% | 1_3339del;4225C>T;4233T>A |
| 5 | human | 55023 | PHIP | pleckstrin homology domain ... | NM_017934.7 | 38.7% | 38.8% | 1_3342del;4228C>T;4236T>A |
| 6 | mouse | 83946 | Phip | pleckstrin homology domain ... | XM_011242840.2 | 49.2% | 50.8% | (many diffs) |
| 7 | mouse | 83946 | Phip | pleckstrin homology domain ... | XM_006511565.3 | 37.8% | 39% | (many diffs) |
| 8 | mouse | 83946 | Phip | pleckstrin homology domain ... | XM_006511564.3 | 34.2% | 35.3% | (many diffs) |
| 9 | mouse | 83946 | Phip | pleckstrin homology domain ... | NM_001081216.1 | 34.2% | 35.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2187
- ORF length:
- 2121
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tccttgggat atggagctta tacctaataa tgctgtattt cctgaagaac 121 taggtaccag tgttccttta actgatggtg agtgcagatc actaatctat aaacctcttg 181 atggagaatg gggtaccaat cccagggatg aagaatgtga aagaattgtg gcaggaataa 241 accagttgat gacactagat attgcctcag catttgtggc ccccgtggat ctgcaagcct 301 atcccatgta ttgcacagta gtggcatatc caacggatct aagtacaatt aaacaaagac 361 tggaaaacag gttttacagg cgggtttctt ccctaatgtg ggaagttcga tatatagagc 421 ataatacacg aacatttaat gagcctggaa gccctattgt gaaatctgct aaattcgtga 481 ctgatcttct tctacatttt ataaaggatc agacttgtta taacataatt ccactttata 541 attcaatgaa gaagaaagtt ttgtctgatt ctgaggatga agagaaagat gctgatgtgc 601 caggaacttc tactcgaaaa aggaaggacc atcagcctag aagaagatta cgtaatagag 661 cccagtctta cgatattcaa gcatggaaga aacagtgtga agaattgtta aatctcatat 721 ttcaatgtga agattcagag cctttccgtc agccggtaga tctccttgaa tatccagact 781 acagagacat cattgacact ccaatggatt ttgctaccgt tagagaaact ttagaggctg 841 ggaattatga gtcaccaatg gagttatgta aagatgtcag acttattttc agtaattcca 901 aagcatatac accaagcaaa agatcaagga tttacagcat gagtttgcgc ttgtctgcat 961 tctttgaaga acacattagt tcagttttat cagattataa atctgctctt cgttttcata 1021 aaagaaatac cataaccaaa aggaggaaga aaagaaacag aagcagctct gtttccagta 1081 gtgctgcatc aagccctgaa aggaaaaaaa ggatcttaaa accccagcta aaatcagaaa 1141 gctctacctc tgcattctct acacctacac gatcaatacc gccaagacac aatgctgctc 1201 agataaacgg taaaacagaa tctagttctg tggttcgaac cagaagcaac cgagtggttg 1261 tagatccagt tgtcactgag caaccatcta cttcttcagc tgcaaagact tttattacaa 1321 aagctaatgc atctgcaata ccagggaaaa caatactaga gaattctgtg aaacattcca 1381 aagctttgaa tactctttcc agtcctggtc aatccagttt tagtcatggc actaggaata 1441 attctgcaaa agaaaacatg gaaaaggaaa agccagtcaa acgtaaaatg aagtcatctg 1501 tactcccaaa ggcgtccact ctttcaaagt catcagctgt cattgagcaa ggagattgta 1561 agaacaacgc tcttgtacca ggaaccattc aagtaaatgg ccatggagga cagccatcaa 1621 aacttgtgaa gaggggacct ggaaggaaac ctaaagtaga agttaatacc aatagtggtg 1681 aaattataca caagaaaagg ggtagaaagc ccaaaaagct acagtatgca aagccagaag 1741 atttagagca aaataatgtg caTCCCATCA GAGATGAAGT ACTTCCTTCT TCAACATGCA 1801 ATTTTCTTTC TGAAACTAAT AATGTAAAGG AAGATTTGTT ACAGAAAAAG AATCGTGGAG 1861 GTAGGAAGCC CAAAAGGAAG ATGAAGACAC AAAAATTAGA TGCAGATCTC CTAGTCCCTG 1921 CAAGTGTCAA AGTGTTAAGG AGAAGTAACC GAAAAAAGAT AGATGATCCT ATAGATGAGG 1981 AAGAAGAGTT TGAAGAACTC AAAGGCTCTG AACCCCACAT GAGAACTAGA AATCAAGGTC 2041 GAAGGACAGC TTTCTATAAT GAGGATGACT CTGAAGAGGA GCAAAGGCAG CTGTTGTTCG 2101 AAGACACCTC TTTAACTTTT GGAACTTCTA GTAGAGGACG AGTCCGAAAG TTGACTGAAA 2161 AAGCAAAAGC TAATTTAATT GGTTGGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2221 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2281 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGTGATCT 2341 GCATAGGTAA TGGCGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2401 aagatt