Transcript: Human XM_017011073.1

PREDICTED: Homo sapiens arginyl-tRNA synthetase 2, mitochondrial (RARS2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RARS2 (57038)
Length:
2516
CDS:
985..2226

Additional Resources:

NCBI RefSeq record:
XM_017011073.1
NBCI Gene record:
RARS2 (57038)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017011073.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000419542 ATCGAATGGACAAGTATAATT pLKO_005 1454 CDS 100% 15.000 21.000 N RARS2 n/a
2 TRCN0000045347 ACCCAGGCATATCGTCAGTTA pLKO.1 2010 CDS 100% 4.950 6.930 N RARS2 n/a
3 TRCN0000045343 GCGTTCTACCATCATAGGAAA pLKO.1 894 5UTR 100% 4.950 6.930 N RARS2 n/a
4 TRCN0000045345 CCTTTGGAGTAGTACAGGGAA pLKO.1 1586 CDS 100% 2.640 3.696 N RARS2 n/a
5 TRCN0000422680 ACAAGCGTCTGGGAGTATATT pLKO_005 1226 CDS 100% 15.000 10.500 N RARS2 n/a
6 TRCN0000434533 ACAGAACATGGCTTCAATTAA pLKO_005 1674 CDS 100% 15.000 10.500 N RARS2 n/a
7 TRCN0000430156 ACTTCAAAGGTTTACTCTTAT pLKO_005 1766 CDS 100% 13.200 9.240 N RARS2 n/a
8 TRCN0000413318 AGCTTTAGGACATCAAGTAAT pLKO_005 936 5UTR 100% 13.200 9.240 N RARS2 n/a
9 TRCN0000416164 CAATATCTGCAGTTCCAATTT pLKO_005 539 5UTR 100% 13.200 9.240 N RARS2 n/a
10 TRCN0000045344 CTCCTCAATTTGTACTGTAAT pLKO.1 1380 CDS 100% 13.200 9.240 N RARS2 n/a
11 TRCN0000174075 CTCCTCAATTTGTACTGTAAT pLKO.1 1380 CDS 100% 13.200 9.240 N RARS2 n/a
12 TRCN0000415333 GACAGTGCTACAACAAGTAAT pLKO_005 756 5UTR 100% 13.200 9.240 N RARS2 n/a
13 TRCN0000045346 GTACTGGTCAAAGGACTGTAA pLKO.1 704 5UTR 100% 4.950 3.465 N RARS2 n/a
14 TRCN0000008902 CCTCCCAAAGTGTTGGGATTA pLKO.1 2415 3UTR 100% 1.080 0.540 Y GPR83 n/a
15 TRCN0000156315 CCTCCCAAAGTGTTGGGATTA pLKO.1 2415 3UTR 100% 1.080 0.540 Y MYORG n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017011073.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08690 pDONR223 100% 68.1% 67.5% None (many diffs) n/a
2 ccsbBroad304_08690 pLX_304 0% 68.1% 67.5% V5 (many diffs) n/a
3 TRCN0000471206 CACCCTGGCCCGAGACGGTGGCAC pLX_317 26.5% 68.1% 67.5% V5 (many diffs) n/a
4 ccsbBroadEn_12325 pDONR223 100% 31.2% 29.4% None 0_1ins525;510_513delGACA;557_1239del n/a
5 ccsbBroad304_12325 pLX_304 0% 31.2% 29.4% V5 0_1ins525;510_513delGACA;557_1239del n/a
6 TRCN0000467868 TCAAGAACTTATCCGTTACTCGCG pLX_317 36.3% 31.2% 29.4% V5 0_1ins525;510_513delGACA;557_1239del n/a
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