Construct: ORF TRCN0000471206

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018515.1_s317c1
Derived from:
ccsbBroadEn_08690
DNA Barcode:
CACCCTGGCCCGAGACGGTGGCAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RARS2 (57038)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471206
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NM_020320.5 99.9% 99.8% 872A>G
2 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NM_001350505.1 97.9% 97.1% (many diffs)
3 human 57038 RARS2 arginyl-tRNA synthetase 2, ... XM_011535949.3 84.8% 81.6% (many diffs)
4 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_134857.1 74.4% (many diffs)
5 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146756.1 73% (many diffs)
6 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146749.1 72% (many diffs)
7 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NM_001318785.1 69.6% 69.5% 0_1ins525;347A>G
8 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NM_001350507.1 69.6% 69.5% 0_1ins525;347A>G
9 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NM_001350508.1 69.6% 69.5% 0_1ins525;347A>G
10 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NM_001350509.1 69.6% 69.5% 0_1ins525;347A>G
11 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NM_001350510.1 69.6% 69.5% 0_1ins525;347A>G
12 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NM_001350511.1 69.6% 69.5% 0_1ins525;347A>G
13 human 57038 RARS2 arginyl-tRNA synthetase 2, ... XM_024446494.1 69.6% 69.5% 0_1ins525;347A>G
14 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146738.1 69.4% (many diffs)
15 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146743.1 68.8% (many diffs)
16 human 57038 RARS2 arginyl-tRNA synthetase 2, ... XR_001743517.2 68.7% (many diffs)
17 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146740.1 68.6% (many diffs)
18 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146748.1 68.4% (many diffs)
19 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NM_001350506.1 68.1% 67.5% (many diffs)
20 human 57038 RARS2 arginyl-tRNA synthetase 2, ... XM_017011073.1 68.1% 67.5% (many diffs)
21 human 57038 RARS2 arginyl-tRNA synthetase 2, ... XM_017011074.2 68.1% 67.5% (many diffs)
22 human 57038 RARS2 arginyl-tRNA synthetase 2, ... XM_017011075.2 68.1% 67.5% (many diffs)
23 human 57038 RARS2 arginyl-tRNA synthetase 2, ... XM_017011076.2 68.1% 67.5% (many diffs)
24 human 57038 RARS2 arginyl-tRNA synthetase 2, ... XM_017011077.2 68.1% 67.5% (many diffs)
25 human 57038 RARS2 arginyl-tRNA synthetase 2, ... XM_017011078.2 68.1% 67.5% (many diffs)
26 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146751.1 66.7% (many diffs)
27 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146757.1 66.6% (many diffs)
28 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146754.1 66.3% (many diffs)
29 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146744.1 66.3% (many diffs)
30 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146747.1 66.2% (many diffs)
31 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146755.1 65.7% (many diffs)
32 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146753.1 65.6% (many diffs)
33 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146750.1 65.6% (many diffs)
34 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146742.1 64.6% (many diffs)
35 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146741.1 64.2% (many diffs)
36 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146752.1 63.4% (many diffs)
37 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146739.1 63.2% (many diffs)
38 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146758.1 62.9% (many diffs)
39 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146746.1 61.1% (many diffs)
40 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146745.1 60.3% (many diffs)
41 human 57038 RARS2 arginyl-tRNA synthetase 2, ... NR_146759.1 60.2% (many diffs)
42 mouse 109093 Rars2 arginyl-tRNA synthetase 2, ... NM_181406.3 85.9% 86.3% (many diffs)
43 mouse 109093 Rars2 arginyl-tRNA synthetase 2, ... XR_390310.3 78.9% (many diffs)
44 mouse 109093 Rars2 arginyl-tRNA synthetase 2, ... XM_006537548.3 51.6% 52.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1800
ORF length:
1734
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gtgcggcttt cgccgcgcta ttgcttgcca gctttccaga gtgttgaatc
121ttccaccaga aaacttgatc acatcaatat ctgcagttcc aatttcccaa aaagaagaag
181tagctgattt tcagctttct gtggattctt tattggaaaa agacaatgac cattcaagac
241cagatattca agttcaagcc aagagactag cagagaagct aagatgtgat acagtggtga
301gtgaaatcag tactggtcaa aggactgtaa atttcaaaat aaacagagag ctcttaacaa
361agacagtgct acaacaagta attgaagatg gctcaaaata tggattaaaa agtgaacttt
421tctctggact tccccagaag aagattgtgg ttgaattcag ttcacctaat gttgccaaaa
481aatttcatgt tggacatttg cgttctacca tcataggaaa ttttatagca aatctcaaag
541aagctttagg acatcaagta ataagaataa attaccttgg cgattggggc atgcagtttg
601gtcttctggg aactggcttc cagctgtttg gctatgagga aaaactgcag tccaatcctc
661tacagcatct ctttgaagtt tatgtacaag ttaataaaga agcagcagat gataaaagtg
721tagcaaaagc agcacaggag ttcttccaac gattggaact gggcgatgtg caagcacttt
781cactgtggca aaaatttcgg gacttgagca ttgaagagta cattcgggtt tacaagcgtc
841tgggagtata ttttgatgaa tattcaggag aatcatttta tcgtgaaaaa tctcaagagg
901tcttaaagtt gctggagagt aaaggactcc tactgagaac aataaaagga acggctgtag
961tagatctctc tgggaatggc gacccctcct caatttgtac tgtaatgcga agtgatggga
1021cttctctcta tgcaaccaga gatcttgcag ctgctataga tcgaatggac aagtataatt
1081ttgatacaat gatatatgtg acagataaag gacaaaaaaa gcattttcag caagtattcc
1141aaatgctgaa gatcatggga tatgactggg cagaaaggtg ccagcacgtg ccctttggag
1201tagtacaggg aatgaagact cgaagaggag atgtcacttt cctggaagat gttttaaatg
1261agattcaatt aaggatgcta cagaacatgg cttcaattaa gacaactaaa gaactcaaga
1321acccacaaga gactgcagag agggTCGGGC TCGCAGCACT CATTATTCAG GACTTCAAAG
1381GTTTACTCTT ATCTGACTAC AAGTTCAGCT GGGATCGTGT TTTCCAGAGT CGCGGGGACA
1441CAGGAGTCTT CCTACAGTAC ACACACGCCC GCCTCCACAG TTTGGAAGAG ACTTTTGGAT
1501GTGGGTACCT GAATGACTTC AACACTGCTT GTTTACAAGA GCCACAGTCT GTTTCAATTC
1561TTCAGCATCT TCTCAGGTTC GACGAGGTGC TTTATAAATC ATCTCAGGAC TTTCAACCCA
1621GGCATATCGT CAGTTACCTT CTAACTTTAA GTCATCTTGC AGCTGTGGCA CACAAAACAC
1681TACAAATAAA AGATAGTCCT CCTGAAGTGG CTGGGGCCAG ACTTCATCTT TTCAAAGCTG
1741TCCGTTCTGT CCTAGCCAAT GGAATGAAAC TTCTTGGAAT AACACCTGTA TGTAGGATGT
1801ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1861GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1921TTTATATATC TTGTGGAAAG GACGACACCC TGGCCCGAGA CGGTGGCACA CGCGTTAAGT
1981Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)