Transcript: Human XM_017012315.1

PREDICTED: Homo sapiens membrane palmitoylated protein 6 (MPP6), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MPP6 (51678)
Length:
8269
CDS:
175..1797

Additional Resources:

NCBI RefSeq record:
XM_017012315.1
NBCI Gene record:
MPP6 (51678)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148238 GTAATGGTATCTCTATAACT pXPR_003 TGG 624 38% 5 0.9492 PALS2 MPP6 76773
2 BRDN0001144856 TGGTATTCACAAAAGAGCTG pXPR_003 GGG 409 25% 4 0.3876 PALS2 MPP6 76774
3 BRDN0001146073 GGGGGAATGATAGATCGACA pXPR_003 AGG 494 30% 5 0.2971 PALS2 MPP6 76775
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017012315.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000273364 TACTTCATGTGGGAGATATAA pLKO_005 677 CDS 100% 15.000 10.500 N MPP6 n/a
2 TRCN0000000303 ACCTCCATCAAGCCCAGAAAT pLKO.1 492 CDS 100% 13.200 9.240 N MPP6 n/a
3 TRCN0000273423 ACCTCCATCAAGCCCAGAAAT pLKO_005 492 CDS 100% 13.200 9.240 N MPP6 n/a
4 TRCN0000380043 ATTACTCCTCAACAGGTATTT pLKO_005 811 CDS 100% 13.200 9.240 N MPP6 n/a
5 TRCN0000273422 CATCACAGATAGAAGATTATC pLKO_005 1875 3UTR 100% 13.200 9.240 N MPP6 n/a
6 TRCN0000379547 GATTCAGTAAGGTTAACAATG pLKO_005 1799 3UTR 100% 10.800 7.560 N MPP6 n/a
7 TRCN0000000302 GTGGCAGAATTGGTTGGTATA pLKO.1 403 CDS 100% 10.800 7.560 N MPP6 n/a
8 TRCN0000273424 GTGGCAGAATTGGTTGGTATA pLKO_005 403 CDS 100% 10.800 7.560 N MPP6 n/a
9 TRCN0000000301 CTGCAACAAATAAACCTTCTA pLKO.1 1843 3UTR 100% 4.950 3.465 N MPP6 n/a
10 TRCN0000000305 GTTAGAAGAGACTGGGACAAT pLKO.1 1033 CDS 100% 4.950 2.970 N MPP6 n/a
11 TRCN0000273366 GTTAGAAGAGACTGGGACAAT pLKO_005 1033 CDS 100% 4.950 2.970 N MPP6 n/a
12 TRCN0000000304 TGTGGCAGAATTGGTTGGTAT pLKO.1 402 CDS 100% 4.950 2.970 N MPP6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017012315.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489158 ACGGGAGACCATCTCCGGCAAGCG pLX_317 23.5% 99.9% 100% V5 (not translated due to prior stop codon) 741G>A n/a
2 TRCN0000489366 GCTCAAATGCGGCGGACAGGTGTC pLX_317 21.6% 99.8% 99.8% V5 741G>A;1620_1621insG n/a
3 TRCN0000474714 TCGAAACATACATTCCTTTGAAAG pLX_317 100% 25.4% 1.2% V5 (not translated due to prior stop codon) 1_1207del n/a
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