Construct: ORF TRCN0000474714
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018852.3_s317c1
- Derived from:
- ccsbBroadEn_15071
- DNA Barcode:
- TCGAAACATACATTCCTTTGAAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MPP6 (51678)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474714
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51678 | MPP6 | membrane palmitoylated prot... | XM_005249775.5 | 32.1% | 1.6% | 1_871del |
| 2 | human | 51678 | MPP6 | membrane palmitoylated prot... | NM_001303037.2 | 25.4% | 1.2% | 1_1207del |
| 3 | human | 51678 | MPP6 | membrane palmitoylated prot... | NM_016447.4 | 25.4% | 1.2% | 1_1207del |
| 4 | human | 51678 | MPP6 | membrane palmitoylated prot... | XM_006715738.3 | 25.4% | 1.2% | 1_1207del |
| 5 | human | 51678 | MPP6 | membrane palmitoylated prot... | XM_006715739.3 | 25.4% | 1.2% | 1_1207del |
| 6 | human | 51678 | MPP6 | membrane palmitoylated prot... | XM_006715740.3 | 25.4% | 1.2% | 1_1207del |
| 7 | human | 51678 | MPP6 | membrane palmitoylated prot... | XM_011515425.2 | 25.4% | 1.2% | 1_1207del |
| 8 | human | 51678 | MPP6 | membrane palmitoylated prot... | XM_017012315.1 | 25.4% | 1.2% | 1_1207del |
| 9 | human | 51678 | MPP6 | membrane palmitoylated prot... | XM_017012316.2 | 25.4% | 1.2% | 1_1207del |
| 10 | human | 51678 | MPP6 | membrane palmitoylated prot... | XM_017012317.1 | 25.4% | 1.2% | 1_1207del |
| 11 | mouse | 56524 | Mpp6 | membrane protein, palmitoyl... | NM_019939.2 | 23% | 1.2% | (many diffs) |
| 12 | mouse | 56524 | Mpp6 | membrane protein, palmitoyl... | NM_001164733.1 | 22.4% | 1.2% | (many diffs) |
| 13 | mouse | 56524 | Mpp6 | membrane protein, palmitoyl... | NM_001164734.1 | 22.4% | 1.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 195
- ORF length:
- 102
- Sequence:
-
1 tcttccattt caggtgtcgt gaGGCCAGGC ATATAAGTTT GTGTCACGAT CTGAGATGGA 61 AGCAGATATT AAAGCTGGAA AGTATTTGGA ACATGGGGAA TATGAAGGAA ATCTCTATGG 121 AACCAAAATT GATTCTATTC TTGAGGTTGT CCAAACTGGA CGGACTTGCA TTCTGGATGT 181 CAACCCACAA GCACTGAAAG TATTGAGGAC ATCAGAGTTT ATGCCCTATG TGGTATTTAT 241 TGCGGCTCCG GAGCTAGAGA CGTTACGTGC CATGCACAAG GCTGTGGTGG ATGCAGGAAT 301 CACTACCAAG CTTCTGACCG ACTCTGACTT GAAGAAAACA GTGGATGAAA GTGCACGGAT 361 TCAGAGAGCA TACAACCACT ATTTTGATTT GATCATCATA AATGATAATC TAGACAAAGC 421 CTTTGAAAAA CTGCAAACTG CCATAGAGAA ACTGAGAATG GAACCACAGT GGGTCCCAAT 481 CAGCTGGGTT TACTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 541 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 601 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CGAAACATAC ATTCCTTTGA 661 AAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att